Phylogenetic distribution and predominant genotype of the avian infectious bronchitis virus in China during 2008-2009

Ji, Jun; Xie, Jingwei; Chen, Feng; Shu, Dingming; Zuo, Kejing; Xue, Chunyi; Qin, Jian-Ping; Li, Hongmei; Bi, Yingzuo; Ma, Jingyun; Xie, Qingmei

doi:10.1186/1743-422x-8-184

Cited by 48 publications

(52 citation statements)

References 39 publications

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“…The closest relative genotype was IBV LX4, the most predominant variant in China , which shared approximately 87% nucleotide and amino acid identities with isolate ck/CH/LGX/ 111119. There are several publications of the IBV genotype that included ck/CH/LGX/111119, but unfortunately these reports do not agree on genotype designations, such as A2 by Ji et al (2011) and CH III by Luo et al (2012) and Feng et al (2014). Also, the virus genotype designations were inconsistent even in reports by the same research group (Ji et al, 2011;Feng et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Identification and molecular characterization of a novel serotype infectious bronchitis virus (GI-28) in China

Chen

Jiang

Zhao

et al. 2017

Veterinary Microbiology

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Avian infectious bronchitis coronavirus (IBV) is a major poultry pathogen. A characteristic feature of IBV is the occurrence of many different strains belonging to different serotypes, which renders complete control of the disease by vaccination a challenging task due to the poor cross-protection between different serotypes. In this study, based on the results of S1 sequence analysis and virus cross-neutralization tests, IBV strain ck/CH/LGX/111119 was found to be genetically and antigenically different from other known IBV types, representing not only a novel genotype, but also a novel serotype (designated as GI-28). Viruses belonging to this novel serotype have been isolated from several regions in China in recent years, suggesting endemic circulation of the serotype in various geographic locations in China. Further studies by complete genomic analysis showed that strain ck/CH/LGX/111119 may have originated from recombination events involving LX4 genotype IBVs and an as-yet-unidentified IBV donating a S1 gene, or from the result of accumulation of mutations and selections, especially in the S1 gene, from a LX4 genotype virus. ck/CH/LGX/111119 is a nephropathogenic strain, although it had broader tissue tropism (respiratory, digestive, urinary, and reproductive tracts) among chickens challenged at one day old. Infection of the oviducts with ck/CH/LGX/111119 found in this study may have severe implications because the virus will likely induce the occurrence of false layers.

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Section: Discussionmentioning

confidence: 99%

Identification and molecular characterization of a novel serotype infectious bronchitis virus (GI-28) in China

Chen

Jiang

Zhao

et al. 2017

Veterinary Microbiology

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“…However, different strains of IBV have been circulating in the Middle East. For example, IBV variants like Q1 (CK/CH/LDL97I/97) (1,11,14,17,20,26,27), IBV variant-1 (793/B, IS/222/96, IS/251/ 96, and IS/64714/96), IBV variant-2 (IS/223/96, IS/572/98, IS/ 585/98, and IS/589/98), Egypt/Beni-Seuf/01, Sul/01/09, D274, and IS/885/00 as well as vaccine strains derived from Massachusetts and 4/91, (8,18,19,24,25) have been detected.…”

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confidence: 99%

Phylogeny and S1 Gene Variation of Infectious Bronchitis Virus Detected in Broilers and Layers in Turkey

et al. 2016

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SUMMARY. The avian coronavirus infectious bronchitis virus (AvCoV-IBV) is recognized as an important global pathogen because new variants are a continuous threat to the poultry industry worldwide. This study investigates the genetic origin and diversity of AvCoV-IBV by analysis of the S1 sequence derived from 49 broiler flocks and 14 layer flocks in different regions of Turkey. AvCoV-IBV RNA was detected in 41 (83.6%) broiler flocks and nine (64.2%) of the layer flocks by TaqMan real-time RT-PCR. In addition, AvCoV-IBV RNA was detected in the tracheas 27/30 (90%), lungs 31/49 (62.2%), caecal tonsils 7/22 (31.8%), and kidneys 4/49 (8.1%) of broiler flocks examined. Pathologic lesions, hemorrhages, and mononuclear infiltrations were predominantly observed in tracheas and to a lesser extent in the lungs and a few in kidneys. A phylogenetic tree based on partial S1 sequences of the detected AvCoV-IBVs (including isolates) revealed that 1) viruses detected in five broiler flocks were similar to the IBV vaccines Ma5, H120, M41; 2) viruses detected in 24 broiler flocks were similar to those previously reported from Turkey and to Israel variant-2 strains; 3) viruses detected in seven layer flocks were different from those found in any of the broiler flocks but similar to viruses previously reported from Iran, India, and China (similar to Israel variant-1 and 4/91 serotypes); and 4) that the AVCoV-IBV, Israeli variant-2 strain, found to be circulating in Turkey appears to be undergoing molecular evolution. In conclusion, genetically different AvCoV-IBV strains, including vaccine-like strains, based on their partial S1 sequence, are circulating in broiler and layer chicken flocks in Turkey and the Israeli variant-2 strain is undergoing evolution.RESUMEN. Análisis filogenético y variación del gene S1 de virus de la bronquitis infecciosa detectados en pollos y gallinas de postura en Turquía.El coronavirus aviar conocido como el virus de la bronquitis infecciosa aviar (AvCoV-IBV), es reconocido como un patógeno global importante debido a que las nuevas variantes son una amenaza continua para la industria avícola en todo el mundo. En este estudio se investigó el origen genético y la diversidad de los virus de bronquitis de bronquitis mediante el análisis de la secuencia del gene S1 de virus derivados de 49 lotes de pollos y de14 lotes de ponedoras en diferentes regiones de Turquía. Se detectó ARN del virus de la bronquitis infecciosa en 41 (83.6%) lotes de pollos y en nueve (64.2%) de los lotes de ponedoras por un método de transcripción reversa y PCR en tiempo con sondas TaqMan. Además, se detectó ARN de virus de la bronquitis infecciosa en las tráqueas 27/30 (90%), los pulmones 31/49 (62.2%), las tonsilas cecales 7/22 (31.8%), y riñones 4/49 (8.1%) de las parvadas de pollo de engorde examinadas. Se observaron lesiones patológicas, hemorragias, e infiltraciones mononucleares principalmente en las tráqueas, en menor medida en los pulmones y en algunos riñones. Un árbol filogenético basado en las secuencias parciales del gene ...

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“…The disease is difficult to be controlled by IBV vaccines, which have been used widely in different chicken farms, because of the rapid circulation and complicated evolution of IBV strains. The chicken flocks have potential danger of IB outbreak when the serotype/genotype of vaccine strains are inconsistent to infective strains [20][21][22][23][24] . Therefore, it is important to choose a suitable IBV candidate vaccine strain to prevent the disease based on profound understanding of IBV epidemiology in China.…”

Section: Discussionmentioning

confidence: 99%

“…A vaccine programme with live-attenuated and inactivated vaccines of Massachusetts (Mass) serotype has been carried out widely and is partially successful in China. But in recent years, the phenomenon of IBV outbreaks occurred frequently in different vaccinated and non-vaccinated chicken flocks showed that the vaccines had poor or no protection against field virus [20][21][22][23][24] . Bing et al [25] found that the proportion of nephro-pathogenic type IBV is higher than any other types by analysis of the strains isolated from China in these years.…”

Section: Introductionmentioning

confidence: 99%

Çin’de Avian Enfeksiyöz Bronşitis Virüsünün Epidemiyolojisi ve Evrimsel Özellikleri

Wang¹,

Gao²,

Zheng³

et al. 2015

Kafkas Univ Vet Fak Derg

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To examine the epidemiology and evolutionary characteristics of avian infectious bronchitis virus (IBV) in mainland China, the S1 gene of 63 IBVs isolated after 1993 was amplified by reverse transcription (RT)-polymerase chain reaction (PCR) and sequenced. Our results showed that the sequence length of 63 IBV strains ranged from 1.608 to 1.635 nucleotide (nt). Compared to the published representative strains, the homology of S1 nt sequences and their deduced amino acid (aa) residues between these isolates and reference strains ranged from 74.6% to 99.9% and 71.7% to 100%, respectively. Analysis of S proteins demonstrated that 63 isolates had eight kinds of cleavage sites (RRFRR, RRIRR, RRSKR, RRTGR, RRHRR, HRRRR, RRSRR and RRLRR), and the amount of RRFRR and HRRRR reached 46. Furthermore, the study showed that there was no direct relation between cleavage sites and genotypes, and cleavage sites were unable to decide the pathology types. Compared to vaccine strain H120, S1 nt sequences of 63 isolates had four sites deleted or inserted frequently, and the S1 proteins had three hypervariable regions. A comprehensive study was also carried out to study the S1 nt sequences of 63 isolates, 210 published reference strains and 10 vaccine strains; the investigation showed that all these stains could be divided into two types including 11 kinds of genotypes by the phylogenetic analysis. However, 63 isolates belonged to Mass, American, LX4, LHLJ/95 I, LDT3/03, J, BJ and LDL/97 I genotypes, and the LX4 genotype was co-circulated dominantly in chicken flocks over an 18-year period. It indicated that there were several IBV genotypes with new changes circulating in China, emphasizing the importance of continued IBV surveillance. Çin'de Avian Enfeksiyöz Bronşitis Virüsünün Epidemiyolojisi ve Evrimsel Özellikleri Özet Avian Enfeksiyöz Bronşitis Virüsünün (IBV) epidemiyolojisini ve evrimsel ezelliklerini araştırmak amacıyla Çin'de 1993 yılından sonra izole edilen 63 IBV S1 geni ters transkriptaz (RT)-polimeraz zincir reaksiyonu (PCR) ile amlifiye edilerek sekansı yapıldı. Çalışma sonuçları 63 IBV suşunun sekans boyutunun 1.608 ile 1.635 nükleotide (nt) arasında değiştiğini gösterdi. Yayınlanmış benzer suşlar ile karşılaştırıldığında bu izolatlar ile referans suşları arasında S1 nt sekanslarının homolojisi ve açığa çıkan amino asit (aa) rezidüleri sırasıyla %74.6 ile %99.9 ve %71.7 ile %100 arasında değişti. S proteinlerinin analizi 63 izolatın 8 çeşit bölünme bölgesine (RRFRR, RRIRR, RRSKR, RRTGR, RRHRR, HRRRR, RRSRR ve RRLRR) sahip olduğunu ve RRFRR ve HRRRR miktarının 46'ya ulaştığını ortaya koydu. Ayrıca, bölünme bölgesi ile genotipler arasında doğrudan bir ilişki olmadığı ve bölünme bölgelerinin patoloji tiplerini belirlemediği tespit edildi. Aşı suşu H120 ile karşılaştırıldığında 63 izolatın S1 nt sekanslarının sıklıkla çıkarılmış veya eklenmiş 4 bölgeye sahip olduğu belirlendi. S1 proteinleri üç adet oldukça değişken bölgeye sahip olduğu gözlemlendi. 63 izolatın S1 nt sekansları, 210 yayınlanmış referans suşu ve 10 aşı suşu...

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Phylogenetic distribution and predominant genotype of the avian infectious bronchitis virus in China during 2008-2009

Cited by 48 publications

References 39 publications

Identification and molecular characterization of a novel serotype infectious bronchitis virus (GI-28) in China

Identification and molecular characterization of a novel serotype infectious bronchitis virus (GI-28) in China

Phylogeny and S1 Gene Variation of Infectious Bronchitis Virus Detected in Broilers and Layers in Turkey

Çin’de Avian Enfeksiyöz Bronşitis Virüsünün Epidemiyolojisi ve Evrimsel Özellikleri

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