Phylogenetic analysis of S1 gene of infectious bronchitis virus reveals emergence of new genotype

Jain, Sudhir; Jain, Hemlata; Bedekar, Megha Kadam

doi:10.5958/2230-732x.2016.00093.0

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The rapid evolution of IBV occurs through two mechanisms, including mutation and recombination (Lim et al, 2011;Selim K. et al, 2014). Mutations are the result from the amino acid substitutions, insertions and deletions frequently observed in the S1 proteins caused by the infidelity of the virus RNA polymerase (Yan et al, 2011;Jain et al, 2016). Recombination, resulting from RNA template switching occurred during RNA replication, has been frequently detected in multiple structural genes, particularly in the S1 gene (Ammayappan et al, 2008).…”

Section: Molecular Mechanisms Of Ibv Evolutionmentioning

confidence: 99%

Genetic Characterization of S1 Gene of Infectious Bronchitis Virus Isolated From Chickens in Thailand During 2014-2016

Munyahongse

View full text Add to dashboard Cite

Infectious bronchitis (IB), caused by infectious bronchitis virus (IBV), is a highly contagious respiratory disease in chickens, causing significant economic losses in poultry industry worldwide. IBV has a high frequency of genetic mutation and recombination in its genome, particularly in the S1 gene, resulting in the emergence of several new IBV variants. This potentially affects the effectiveness of currently used IBV vaccine. Therefore, the continuous monitoring of the currently circulating IBV strains is essential. In this study, the genetic characteristic of IBV strains circulating in Thai chicken flocks during 2014-2016 was investigated by analysis of the complete S1 gene. A total of 629 (37.7%) out of 1,668 samples, collected from all chicken-raising regions of Thailand, was positive for IBV by S1-specific RT-PCR. The results showed that IBV was widely distributed in all chicken-raising regions of Thailand. Among the IBV positive samples collected in this study, 150 IBVs were randomly selected for complete S1 gene sequencing and analysis. The results based on the phylogenetic analysis of the complete S1 gene revealed that the 2014-2016 Thai IBVs characterized in this study were clustered into five genotypes, including QX-like IBV (n=129), Massachusetts (n=12), 4/91 (n=5), Connecticut (n=2) and a novel IBV genotype (n=2). The results demonstrated that QX-like IBV has become the predominant genotype currently circulating in Thailand. Recombination analysis of the S1 gene of the 2014-2016 Thai IBVs showed the emergence of a novel recombinant IBV genotype originating from Thai QX-like IBV and 4/91 vaccine strain, which was first identified in this study. Overall, the results from this study indicate that the genetic characteristic of Thai IBVs has changed when compared to the characteristic described in previous reports, suggesting the continuing evolution of IBV in Thailand. Therefore, this study highlights the importance of continuous IBV surveillance in chickens for effective control and prevention of IB.

show abstract

Section: Molecular Mechanisms Of Ibv Evolutionmentioning

confidence: 99%

Genetic Characterization of S1 Gene of Infectious Bronchitis Virus Isolated From Chickens in Thailand During 2014-2016

Munyahongse

View full text Add to dashboard Cite

show abstract

Phylogenetic analysis of S1 gene of infectious bronchitis virus reveals emergence of new genotype

Cited by 1 publication

References 0 publications

Genetic Characterization of S1 Gene of Infectious Bronchitis Virus Isolated From Chickens in Thailand During 2014-2016

Genetic Characterization of S1 Gene of Infectious Bronchitis Virus Isolated From Chickens in Thailand During 2014-2016

Contact Info

Product

Resources

About