2017
DOI: 10.1016/j.meegid.2017.09.011
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Abstract: Feline immunodeficiency virus (FIV), like other retroviruses, displays large genomic divergence when different isolates are compared. In this study, 31 FIV positive samples of domestic cats from Porto Alegre, RS, Brazil were used aiming at a detailed genomic characterization and a better understanding of the molecular epidemiology of the virus in Brazil. The proviral env genes were partially amplified, sequenced and compared with another 237 sequences from different continents. We identified several Brazilian … Show more

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Cited by 10 publications
(9 citation statements)
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“…Interestingly, it was observed for FIV that intra-individual rates were not dissimilar to evolution rates that have been reported for populations (i.e., interhost evolution) (20,21,35,36). Follow-up studies comparing intra-individual and population rates could further our understanding of the interplay between intra-individual and interhost phylodynamics.…”
Section: Discussionmentioning
confidence: 85%
“…Interestingly, it was observed for FIV that intra-individual rates were not dissimilar to evolution rates that have been reported for populations (i.e., interhost evolution) (20,21,35,36). Follow-up studies comparing intra-individual and population rates could further our understanding of the interplay between intra-individual and interhost phylodynamics.…”
Section: Discussionmentioning
confidence: 85%
“…The first round of amplification was performed with primers VIF_FIV_PF and ENV_PR [ 18 ] generating a 3.1-kb-long fragment using the Phusion High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA). In order to amplify part of the env gene, a second round of amplification was made with primers ENV2-3_PF and ENV2-3_PR [ 21 ], giving rise to an expected final product of 831 pb. In order to amplify the vif gene, the 3.1 kb was submitted to another second round of amplification with primers VIF_FIV_PF and VIF_FIV_PR [ 18 ], generating a 756-pb-long fragment that encompassed the entire vif gene (sequences of primers are available in the Appendix data, Table A1 ).…”
Section: Methodsmentioning
confidence: 99%
“…Such program identifies G→A hypermutations using the default setting, where hypermutations are detected in a GRD motif (where R is code for G or A, and D for G, A or T), and the context requirements are enforced on query sequences. To select the reference sequence for the analyses of the env gene fragment, a phylogenetic reconstruction was performed using parameters and reference described in our previous work [ 21 ]. Briefly, we submitted our dataset (including 50 sequences from public databases and 27 sequences described in this study) to a maximum-likelihood phylogenetic reconstruction in PhyML.…”
Section: Methodsmentioning
confidence: 99%
“…In North America, FIV clades A, B, and C have been described [15,16,31,33]. In South America, clades A, B, and E have been reported, with clades B and E being predominant and clade E only being described in this geographic area so far [18,28,38,39,40,41]. Limited information on the FIV strains and clades is available for Asia.…”
Section: Introductionmentioning
confidence: 99%