Photothermally triggered nitric oxide nanogenerator targeting type IV pili for precise therapy of bacterial infections

Wu, Yang; Deng, Guiyun; Jiang, Kai; Wang, Huajuan; Song, Zhiyong; Han, Hao

doi:10.1016/j.biomaterials.2020.120588

Cited by 69 publications

(52 citation statements)

References 65 publications

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“…However, it is impractical to heat nanozymes by direct contact with external heat sources, especially for in vivo antibacterial applications [ 23 ]. To overcome these shortcomings, the combination of nanozyme-based catalytic therapy and photothermal therapy (PTT) is a promising approach [ 24 , 25 ]. PTT is an effective method that uses photothermal reagents to generate local high temperature to treat bacterial infections under near-infrared (NIR) light irradiation [ 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Copper single-atom catalysts with photothermal performance and enhanced nanozyme activity for bacteria‐infected wound therapy

Wang

Shi

Zha

et al. 2021

Bioactive Materials

233

184

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Nanozymes have become a new generation of antibiotics with exciting broad-spectrum antibacterial properties and negligible biological toxicity. However, their inherent low catalytic activity limits their antibacterial properties. Herein, Cu single-atom sites/N doped porous carbon (Cu SASs/NPC) is successfully constructed for photothermal-catalytic antibacterial treatment by a pyrolysis-etching-adsorption-pyrolysis (PEAP) strategy. Cu SASs/NPC have stronger peroxidase-like catalytic activity, glutathione (GSH)-depleting function, and photothermal property compared with non-Cu-doped NPC, indicating that Cu doping significantly improves the catalytic performance of nanozymes. Cu SASs/NPC can effectively induce peroxidase-like activity in the presence of H 2 O 2 , thereby generating a large amount of hydroxyl radicals (•OH), which have a certain killing effect on bacteria and make bacteria more susceptible to temperature. The introduction of near-infrared (NIR) light can generate hyperthermia to fight bacteria, and enhance the peroxidase-like catalytic activity, thereby generating additional •OH to destroy bacteria. Interestingly, Cu SASs/NPC can act as GSH peroxidase (GSH-Px)-like nanozymes, which can deplete GSH in bacteria, thereby significantly improving the sterilization effect. PTT-catalytic synergistic antibacterial strategy produces almost 100% antibacterial efficiency against Escherichia coli ( E. coli ) and methicillin-resistant Staphylococcus aureus ( MRSA ). In vivo experiments show a better PTT-catalytic synergistic therapeutic performance on MRSA-infected mouse wounds. Overall, our work highlights the wide antibacterial and anti-infective bio-applications of Cu single-atom-containing catalysts.

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Section: Introductionmentioning

confidence: 99%

Copper single-atom catalysts with photothermal performance and enhanced nanozyme activity for bacteria‐infected wound therapy

Wang

Shi

Zha

et al. 2021

Bioactive Materials

233

184

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“…Preferably, smart photothermal nanoparticles should penetrate deep and evenly over the depth of an infectious biofilm. Most of the targeting moieties involve specific pathogen-recognitions, such as type IV pili or type III secretion protein for targeting Pseudomonas aeruginosa [31,32], vancomycin [33][34][35], anti-protein A IgY [36], endolysins or other specific bacterial receptors for targeting S. aureus, including MRSA strains [37,38]. Ideal therapeutic approaches should be based on non-specific bacterial targeting, at least until pathogen identification has been done in order to save valuable time during life-threatening infections.…”

Section: Discussionmentioning

confidence: 99%

Encapsulation of Photothermal Nanoparticles in Stealth and pH-Responsive Micelles for Eradication of Infectious Biofilms In Vitro and In Vivo

Gao

et al. 2021

Nanomaterials

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Photothermal nanoparticles can be used for non-antibiotic-based eradication of infectious biofilms, but this may cause collateral damage to tissue surrounding an infection site. In order to prevent collateral tissue damage, we encapsulated photothermal polydopamine-nanoparticles (PDA-NPs) in mixed shell polymeric micelles, composed of stealth polyethylene glycol (PEG) and pH-sensitive poly(β-amino ester) (PAE). To achieve encapsulation, PDA-NPs were made hydrophobic by electrostatic binding of indocyanine green (ICG). Coupling of ICG enhanced the photothermal conversion efficacy of PDA-NPs from 33% to 47%. Photothermal conversion was not affected by micellar encapsulation. No cytotoxicity or hemolytic effects of PEG-PAE encapsulated PDA-ICG-NPs were observed. PEG-PAE encapsulated PDA-ICG-NPs showed good penetration and accumulation in a Staphylococcus aureus biofilm. Penetration and accumulation were absent when nanoparticles were encapsulated in PEG-micelles without a pH-responsive moiety. PDA-ICG-NPs encapsulated in PEG-PAE-micelles found their way through the blood circulation to a sub-cutaneous infection site after tail-vein injection in mice, yielding faster eradication of infections upon near-infrared (NIR) irradiation than could be achieved after encapsulation in PEG-micelles. Moreover, staphylococcal counts in surrounding tissue were reduced facilitating faster wound healing. Thus, the combined effect of targeting and localized NIR irradiation prevented collateral tissue damage while eradicating an infectious biofilm.

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“…372 To address these shortcomings, researchers have designed artificial nanozymes for PTTcatalytic antibacterial therapy. [373][374][375] The PTT method employs photothermal reagents to induce a local high temperature under the irradiation of a near-infrared laser to inhibit bacterial infections. 376 However, the therapeutic effects of PTT-catalytic antibacterial therapy are still unsatisfactory owing to the weak catalytic activity of traditional nanozymes.…”

Section: Antibacterialmentioning

confidence: 99%

Engineering single-atom catalysts toward biomedical applications

Chang

Zhang

et al. 2022

Chem. Soc. Rev.

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Photothermally triggered nitric oxide nanogenerator targeting type IV pili for precise therapy of bacterial infections

Cited by 69 publications

References 65 publications

Copper single-atom catalysts with photothermal performance and enhanced nanozyme activity for bacteria‐infected wound therapy

Copper single-atom catalysts with photothermal performance and enhanced nanozyme activity for bacteria‐infected wound therapy

Encapsulation of Photothermal Nanoparticles in Stealth and pH-Responsive Micelles for Eradication of Infectious Biofilms In Vitro and In Vivo

Engineering single-atom catalysts toward biomedical applications

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