Photosynthetic characterization of Rubisco transplantomic lines reveals alterations on photochemistry and mesophyll conductance

Galmés, Jeroni; Perdomo, Juan Alejandro; Flexas, Jaume; Whitney, Spencer M.

doi:10.1007/s11120-013-9848-8

Cited by 24 publications

(16 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is therefore important to measure wild-type leaves of comparable physiological age and development. Galmes et al (2013) reported significantly lower g m values calculated from chlorophyll fluorescence for tob(bid) and tob(flo) compared to wild type. Their plants were grown without CO 2 supplementation, but under similar irradiance, photoperiod, temperature, and humidity to this study’s growth conditions.…”

Section: Discussionmentioning

confidence: 87%

Exploiting transplastomically modified Rubisco to rapidly measure natural diversity in its carbon isotope discrimination using tuneable diode laser spectroscopy

Caemmerer

Tazoe

Evans

et al. 2014

Journal of Experimental Botany

View full text Add to dashboard Cite

Carbon isotope discrimination (Δ) during C3 photosynthesis is dominated by the fractionation occurring during CO2-fixation by the enzyme Rubisco. While knowing the fractionation by enzymes is pivotal to fully understanding plant carbon metabolism, little is known about variation in the discrimination factor of Rubisco (b) as it is difficult to measure using existing in vitro methodologies. Tuneable diode laser absorption spectroscopy has improved the ability to make rapid measurements of Δ concurrently with photosynthetic gas exchange. This study used this technique to estimate b in vivo in five tobacco (Nicotiana tabacum L. cv Petit Havana [N,N]) genotypes expressing alternative Rubisco isoforms. For transplastomic tobacco producing Rhodospirillum rubrum Rubisco b was 23.8±0.7‰, while Rubisco containing the large subunit Leu-335-Val mutation had a b-value of 13.9±0.7‰. These values were significantly less than that for Rubisco from wild-type tobacco (b=29‰), a C3 species. Transplastomic tobacco producing chimeric Rubisco comprising tobacco Rubisco small subunits and the catalytic large subunits from either the C4 species Flaveria bidentis or the C3-C4 species Flaveria floridana had b-values of 27.8±0.8 and 28.6±0.6‰, respectively. These values were not significantly different from tobacco Rubisco.

show abstract

Section: Discussionmentioning

confidence: 87%

Exploiting transplastomically modified Rubisco to rapidly measure natural diversity in its carbon isotope discrimination using tuneable diode laser spectroscopy

Caemmerer

Tazoe

Evans

et al. 2014

Journal of Experimental Botany

View full text Add to dashboard Cite

show abstract

“…The simulated value of C c = 250 mbar is representative of that encountered in the chloroplast stroma of C 3 species under well-watered conditions (Bermúdez et al, 2012;Scafaro et al, 2012;Galmés et al, 2013). Under mild to moderate water stress, when no metabolic impairment is present, the decrease in the stomatal and leaf mesophyll conductances to CO 2 provokes a decrease in the concentration of CO 2 in the chloroplast (Flexas et al, 2006).…”

Section: The Co 2 Assimilation Potential Of Rubisco Kinetics In Cropsmentioning

confidence: 97%

Rubisco Catalytic Properties and Temperature Response in Crops

2016

Self Cite

View full text Add to dashboard Cite

ORCID IDs: 0000-0003-3272-3054 (C.H.-C.); 0000-0001-7966-0295 (M.V.K.); 0000-0002-7299-9349 (J.G.).Rubisco catalytic traits and their thermal dependence are two major factors limiting the CO 2 assimilation potential of plants. In this study, we present the profile of Rubisco kinetics for 20 crop species at three different temperatures. The results largely confirmed the existence of significant variation in the Rubisco kinetics among species. Although some of the species tended to present Rubisco with higher thermal sensitivity (e.g. Oryza sativa) than others (e.g. Lactuca sativa), interspecific differences depended on the kinetic parameter. Comparing the temperature response of the different kinetic parameters, the Rubisco K m for CO 2 presented higher energy of activation than the maximum carboxylation rate and the CO 2 compensation point in the absence of mitochondrial respiration. The analysis of the Rubisco large subunit sequence revealed the existence of some sites under adaptive evolution in branches with specific kinetic traits. Because Rubisco kinetics and their temperature dependency were species specific, they largely affected the assimilation potential of Rubisco from the different crops, especially under those conditions (i.e. low CO 2 availability at the site of carboxylation and high temperature) inducing Rubisco-limited photosynthesis. As an example, at 25°C, Rubisco from Hordeum vulgare and Glycine max presented, respectively, the highest and lowest potential for CO 2 assimilation at both high and low chloroplastic CO 2 concentrations. In our opinion, this information is relevant to improve photosynthesis models and should be considered in future attempts to design more efficient Rubiscos.

show abstract

“…Rubisco activity is highly responsive to temperature changes (Sharkey 2005;Hikosaka et al 2006;Yamori et al 2006;Sage and Kubien 2007), and thus influences the temperature response of net photosynthesis (Yamori et al 2006;Galmés et al 2013). Rubisco's maximum carboxylase turnover rate (k cat c ) increases exponentially with temperature (Sage 2002).…”

Section: Introductionmentioning

confidence: 99%

Temperature dependence of in vitro Rubisco kinetics in species of Flaveria with different photosynthetic mechanisms

et al. 2015

Self Cite

View full text Add to dashboard Cite

There is general consensus in the literature that plants with different photosynthetic mechanisms (i.e. C3 vs. C4) have Rubiscos characterised by different kinetic performances. However, potential differences in the temperature dependencies of Rubisco kinetic parameters between C3 and C4 plants are uncertain. Accordingly, six species of Flaveria with contrasting photosynthetic mechanisms (C3, C3/C4 and C4) were selected and their Rubisco Michaelis-Menten constants for CO2 and RuBP (K c and K RuBP), carboxylase catalytic turnover rate ([Formula: see text]) and CO2/O2 specificity factor (S c/o) were measured between 10 and 40 °C. The results confirmed different Rubisco characteristics between C3 and C4 plants. Rubisco from the C3 species had higher E a for K c and [Formula: see text] than that from C4 species, which were translated into differences in the temperature response of the carboxylase catalytic efficiency ([Formula: see text]/K c). However, E a did not differ for S c/o or K RuBP. Although a mechanism remains uncertain, it appears that the Asp/Glu-149-Ala and Met-309-Ile substitutions lead to differences in the temperature responses of catalysis between C3 and C4 Rubiscos in Flaveria. Therefore, the above observations are consistent with the fact that C3 species have a higher photosynthetic efficiency and ecological dominance in cool environments, with respect to C4 species in temperate environments.

show abstract

Photosynthetic characterization of Rubisco transplantomic lines reveals alterations on photochemistry and mesophyll conductance

Cited by 24 publications

References 69 publications

Exploiting transplastomically modified Rubisco to rapidly measure natural diversity in its carbon isotope discrimination using tuneable diode laser spectroscopy

Exploiting transplastomically modified Rubisco to rapidly measure natural diversity in its carbon isotope discrimination using tuneable diode laser spectroscopy

Rubisco Catalytic Properties and Temperature Response in Crops

Temperature dependence of in vitro Rubisco kinetics in species of Flaveria with different photosynthetic mechanisms

Contact Info

Product

Resources

About