Photosensitizer effects of MWCNTs-COOH particles on CT26 fibroblastic cells exposed to laser irradiation

Abstract

In the current study, photosensitizer effect of carboxylate multiwalled carbon nanotubes (MWCNTs-COOH) on CT26 fibroblastic cells following near infrared (NIR) irradiation was quantized in photothermal therapy (PTT). Moreover, it was tried to achieve optimal dose of MWCNTs-COOH and laser exposure time. Characterizations of MWCNTs-COOH were scrutinized using scanner electron microscope (SEM), spectrophotometer, and particle size analyzer. The seeded CT26 cells were treated with nontoxic concentrations of MWCNTs-COOH and then irradiated. Finally, viability (%) of the CT26 cells was determined using MTT assay. The findings revealed that 10, 50, and 80 lg/mL of MWCNTs-COOH have remarkable photosensitizer effects on CT26 cancerous cell lines against NIR irradiation (2.5 W/cm 2). It was shown that using the 80 lg/mL concentration of MWCNTs-COOH against 60, 120, 180, 240, and 300 s of NIR irradiation and also, 10 and 50 lg/mL concentration of MWCNTs-COOH against 180, 240, and 300 s of NIR irradiation can lead to significant decrease in mean cell viability (%) by more than 50%. According to the obtained data, it seems that using the PPT with MWCNTs-COOH, as adjunct therapy in CT26 fibroblastic cells, can help to increase therapeutic ratio of main modalities of cancer treatment such as radiotherapy, chemotherapy, and surgery.

Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair

“…After CS-GT and L929 cells were co-incubated for 1 and 2 days, CS-GT at a concentration of 100 µg/mL promoted cell growth to some extent, and cell viability values were 112.65% and 112.23%, respectively, indicating that CS-GT at this concentration is non-toxic to L929 cells. After CS-GT and L929 cells were co-incubated for 3 days, cell viability decreased to 90.79%, indicating that CS-GT at this concentration exhibited limited cell inhibition, possibly because cell proliferation via cell division led to higher cell density and competition among cells for nutrition (p < 0.05 compared to days 1 and 2) [33]. For CS-GT at a concentration of 200 µg/mL, cell viability values on days 1 and 2 were 101.68% and 100.44%, respectively, and no significant impact on cell growth was noted.…”

Comprehensive experimental investigation of the effective parameters on stability of silica nanoparticles during low salinity water flooding with minimum scale deposition into sandstone reservoirs

“…ZP is the main parameter in the NPs distribution for the nano-suspensions 38 . When the ZP has sufficient high values (without regard to the negative or positive charges), it can make sure the stability of the NPs in an aqueous solution.…”

“…When the ZP has sufficient high values (without regard to the negative or positive charges), it can make sure the stability of the NPs in an aqueous solution. In contrast, lower ZP values cause NPs agglomeration in aqueous solution 38 , 39 .…”

A Review on Carbon Nanotubes Family of Nanomaterials and Their Health Field

“…Even CNTs only functionalized with −COOH already present remarkable photosensitizer effects and potentially can be used for development of Photothermal therapy (PTT) and Photodynamic therapy (PDT). 99 In this regard, in some alternatives, SWCNTs or MWCNTs have been explored as an adjuvant in photothermal treatments due to their light absorber property, being effective against breast cancer in the PTT by development of the CNTs complex with PEG, leading a suppression of tumor growth and reducing the amount of tumor-induced bone destruction 100 and melanoma tumor size after NIR laser radiation. 101 CNTs were applied in a complex with ruthenium(II) producing reactive oxidative species (ROS) (Figure 6) achieving anticancer efficacies in both in vitro and in vivo models.…”