Photophysical processes of energy conversion in thylakoid membranes of Chlamydomonas reinhardtii mutants D1-R323H, D1-R323D, and D1-R323L

Kukarskikh, G. P.; Zagidullin, V. E.; Antal, Taras K.; Krendeleva, T. E.; Paschenko, V.Z.

doi:10.1134/s1990747810020029

Cited by 1 publication

(1 citation statement)

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“…The absence of the JI phase is often observed in cells with impaired electron flow at the donor or acceptor side of PSII (see e.g. Kukarskikh et al ). A decrease in the fluorescence yield beyond the P peak has been earlier assigned to the initial stage of NPQ (Dau and Hansen ).…”

Section: Discussionmentioning

confidence: 99%

Chlorophyll fluorescence induction and relaxation system for the continuous monitoring of photosynthetic capacity in photobioreactors

Antal

Konyukhov

Волгушева

et al. 2018

Physiologia Plantarum

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The development of high-performance photobioreactors equipped with automatic systems for non-invasive real-time monitoring of cultivation conditions and photosynthetic parameters is a challenge in algae biotechnology. Therefore, we developed a chlorophyll (Chl) fluorescence measuring system for the online recording of the light-induced fluorescence rise and the dark relaxation of the flash-induced fluorescence yield (Qa - re-oxidation kinetics) in photobioreactors. This system provides automatic measurements in a broad range of Chl concentrations at high frequency of gas-tight sampling, and advanced data analysis. The performance of this new technique was tested on the green microalgae Chlamydomonas reinhardtii subjected to a sulfur deficiency stress and to long-term dark anaerobic conditions. More than thousand fluorescence kinetic curves were recorded and analyzed during aerobic and anaerobic stages of incubation. Lifetime and amplitude values of kinetic components were determined, and their dynamics plotted on heatmaps. Out of these data, stress-sensitive kinetic parameters were specified. This implemented apparatus can therefore be useful for the continuous real-time monitoring of algal photosynthesis in photobioreactors.

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Section: Discussionmentioning

confidence: 99%