Photodynamic Therapy with Rose Bengal Induces GroEL Expression in<i>Streptococcus mutans</i>

Bolean, Maytê; Paulino, Tony de Paiva; Thedei, Geraldo; Ciancaglini, Pietro

doi:10.1089/pho.2009.2635

Cited by 26 publications

(11 citation statements)

References 51 publications

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“…To date, several researchers have carried out studies on the PDT effect against streptococcal species associated with dental caries and showed the possibility for targeting these cariogenic bacteria. 2,13,18,19,20,21,22,23 However, the present study is the first to show yet reported a PDT effect on S. mutans using erythrosine and a dental halogen curing unit.…”

Section: Discussionmentioning

confidence: 54%

The photodynamic therapy on Streptococcus mutans biofilms using erythrosine and dental halogen curing unit

et al. 2012

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The purpose of our study was to evaluate the effect of photodynamic therapy (PDT), using erythrosine as a photosensitizing agent and a dental halogen curing unit as a light source, on Streptococcus mutans in a biofilm phase. The S. mutans biofilms were formed in a 24-well cell culture cluster. Test groups consisted of biofilms divided into four groups: group 1: no photosensitizer or light irradiation treatment (control group); group 2: photosensitizer treatment alone; group 3: light irradiation alone; group 4: photosensitizer treatment and light irradiation. After treatments, the numbers of colony-forming unit (CFU) were counted and samples were examined by confocal laser scanning fluorescence microscopy (CLSM). Only group 4 (combined treatment) resulted in significant increases in cell death, with rates of 75% and 55% after 8 h of incubation, and 74% and 42% at 12 h, for biofilms formed in brain–heart infusion (BHI) broth supplemented with 0% or 0.1% sucrose, respectively. Therefore, PDT of S. mutans biofilms using a combination of erythrosine and a dental halogen curing unit, both widely used in dental clinics, resulted in a significant increase in cell death. The PDT effects are decreased in biofilms that form in the presence of sucrose.

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Section: Discussionmentioning

confidence: 54%

The photodynamic therapy on Streptococcus mutans biofilms using erythrosine and dental halogen curing unit

et al. 2012

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“…The first of these studies was performed by Bolean et al [115]. They tested whether the expression of GroEL by Streptococcus mutans was enhanced after APDI with rose bengal.…”

Section: Mechanisms Determining Bacterial Susceptibility To Apdimentioning

confidence: 99%

Can microbial cells develop resistance to oxidative stress in antimicrobial photodynamic inactivation?

Kashef

Hamblin

2017

Drug Resistance Updates

246

173

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Infections have been a major cause of disease throughout the history of humans on earth. With the introduction of antibiotics, it was thought that infections had been conquered. However, bacteria have been able to develop resistance to antibiotics at an exponentially increasing rate. The growing threat from multi-drug resistant organisms calls for intensive action to prevent the emergence of totally resistant and untreatable infections. Novel, non-invasive, non-antibiotic strategies are needed that act more efficiently and faster than current antibiotics. One promising alternative is antimicrobial photodynamic inactivation (APDI), an approach that produces reactive oxygen species when dyes and light are combined. So far, it has been questionable if bacteria can develop resistance against APDI. This review paper gives an overview of recent studies concerning the susceptibility of bacteria towards oxidative stress, and suggests possible mechanisms of the development of APDI-resistance that should at least be addressed. Some ways to potentiate APDI and also to overcome future resistance are suggested.

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“…toluidine blue and methylene blue) are classified at the blue broadband and are correctly activated by red lights at 600-660nm that corresponds to complementary wavelenght [29,30]. On this same way, red dyes, such as rose bengal, erythrosine, Photogem/Photosan can be irrdiated with wavelenghts between 400-500 nm that corresponds to the blue light spectrum [31,32].…”

Section: Photodynamic Antimicrobial Chemotherapy In Dentistrymentioning

confidence: 99%

Photodynamic and Tissue Tolerable Plasma Therapies as Alternatives to Antimicrobials to Control Pathogenic Biofilms

Paschoal

Dantas

Duarte

2015

Curr Oral Health Rep

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The need for novel antimicrobial techniques has become critical for a number of reasons, including the emergence of resistant strains because of excessive prescription and misuse of antibiotics. The mouth is colonized by a large number of microorganisms. When these microorganisms are organized as biofilms they can contribute to chronic diseases in the human population, such as dental caries, candidiasis, and periodontal disease. Hence, alternative antimicrobial approaches have risen to facilitate the treatment of these diseases. As novel therapies, photodynamic antimicrobial chemotherapy and tissue-tolerable plasma are promising methods that offer the possibility of microbial decontamination with decreased odds for the traditional side effects. Thus, the present review aims to offer an overview and future directions of these new approaches to control pathogenic oral biofilms.

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Photodynamic Therapy with Rose Bengal Induces GroEL Expression inStreptococcus mutans

Abstract: PDT may cause effects similar to those of other stressing conditions in S. mutans, and cell death induced by this treatment reflects its incapacity to protect itself sufficiently against the deleterious effects of PDT with Rose bengal.

Cited by 26 publications

References 51 publications

The photodynamic therapy on Streptococcus mutans biofilms using erythrosine and dental halogen curing unit

The photodynamic therapy on Streptococcus mutans biofilms using erythrosine and dental halogen curing unit

Can microbial cells develop resistance to oxidative stress in antimicrobial photodynamic inactivation?

Photodynamic and Tissue Tolerable Plasma Therapies as Alternatives to Antimicrobials to Control Pathogenic Biofilms

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