Photodynamic efficacy of Rosebengal-gold nanoparticle complex on Vero and HeLa cell lines

Abstract

Photodynamic therapy (PDT) is a promising treatment modality in the management of premalignant and malignant conditions. Nanoparticles were used recently as drug delivery agents and have shown increase in the efficacy of PDT. In this study we have used gold nanoparticles as a potential drug delivery agent in enhancing the photodynamic efficacy. Glutathione (GSH) capped gold nanoparticles were synthesized by immobilizing the thiol group of the GSH on to the surface of the gold nanoparticles. Rosebengal, a hydrophilic photosensitizer was conjugated to the amine group of the bounded GSH. These synthesized rosebengal conjugated gold nanoparticles were characterized using FT-IR spectroscopy for confirmation of the complex formation. Further, studies on the absorption, emission and lifetime of the nanocomplexes-show significant changes due to the formation of the complex. The PDT efficacy of these nanocomplexes and sensitizer alone were studied against Vero and HeLa cell lines. The phototoxicity results shows the nanocomplexes are more phototoxic than that of the free rosebengal where the covalent complex being the better of the nanocomplex.

Porphyrin Photoabsorption and Fluorescence Variation with Adsorptive Loading on Gold Nanoparticles

“…The five absorption maxima of porphyrin adsorbates were red-shifted up to 4 nm ( Supplementary Tables S1 , S2 ) compared with those of the corresponding porphyrin–alkanethiols (1b and 2, Supplementary Figure S1 ). A similar bathochromic effect is also observed in other dye–nanoparticle conjugates ( Prasanna et al, 2014 ; Ashjari et al, 2015 ).…”

Singlet Oxygen Generation Driven by Sulfide Ligand Exchange on Porphyrin–Gold Nanoparticle Conjugates

“…In the UV-vis absorption spectrum, a slight broadening of the Soret band is observed when porphyrin is introduced onto the AuNP surface. Similar broadening is also widely observed in other dye-AuNP conjugates and is mainly attributed to the exciton coupling and restricted molecular motion of the chromophore [29][30][31]. Using this property, the desorption of the porphyrin by the ligand exchange reaction can be monitored by the absorption spectra.…”

Luminescent CaTiO3:Yb,Er nanofibers co-conjugated with Rose Bengal and gold nanorods for potential synergistic photodynamic/photothermal therapy

“…The red shift observed may be attributed to the electron donating nature of the amine group. 31 Therefore, the emission of ~540 nm was strongly Figure 4b, indicating the effective absorption of green emission by RB molecules.…”