Photodynamic antibacterial and antibiofilm activity of RLP068/Cl against Staphylococcus aureus and Pseudomonas aeruginosa forming biofilms on prosthetic material

Vassena, Christian; Fenu, Simone; Giuliani, Francesco; Fantetti, Lia; Roncucci, Gabrio; Simonutti, Giulio; Romanò, Carlo Luca; Francesco, Raffaele De; Drago, Lorenzo

doi:10.1016/j.ijantimicag.2014.03.012

Cited by 59 publications

(40 citation statements)

References 29 publications

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“…Nevertheless these compounds are not highly active, and many laboratories have attempted to introduce compounds that have much higher activity, or alternatively are naturally occurring dyes that are supposed to be easier to get thought regulatory barriers. Notable examples of the synthetic high activity compounds are the cationic zinc phthalocyanine RLP068 [30], the cationic phenalenone derivative known as SAPYR [31], and the porphyrin known as Sylsens B [32]. Examples of compounds derived from natural products are a cationic hypericin compound derived from St John's Wort [33], a cationic riboflavin compound derived from vitamin B2 [34], and a cationic derivative of curcumin (a yellow spice found in turmeric) known as SACUR-3 [35].…”

Section: New Photosensitizersmentioning

confidence: 99%

Antimicrobial photodynamic inactivation: a bright new technique to kill resistant microbes

Hamblin

2016

Current Opinion in Microbiology

595

342

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Photodynamic therapy (PDT) uses photosensitizers (non-toxic dyes) that are activated by absorption of visible light to form reactive oxygen species (including singlet oxygen) that can oxidize biomolecules and destroy cells. Antimicrobial photodynamic inactivation (aPDI) can treat localized infections. aPDI neither causes any resistance to develop in microbes, nor is affected by existing drug resistance status. We discuss some recent developments in aPDI. New photosensitizers including polycationic conjugates, stable synthetic bacteriochlorins and functionalized fullerenes are described. The microbial killing by aPDI can be synergistically potentiated (several logs) by harmless inorganic salts via photochemistry. Genetically engineered bioluminescent microbial cells allow PDT to treat infections in animal models. Photoantimicrobials have a promising future in the face of the unrelenting increase in antibiotic resistance.

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Section: New Photosensitizersmentioning

confidence: 99%

Antimicrobial photodynamic inactivation: a bright new technique to kill resistant microbes

Hamblin

2016

Current Opinion in Microbiology

595

342

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“…The quantitative analysis of biofilms production was performed by counting the number of bacterial colonies grown on the Nutrient Agar (NA) plates. All the determinations were repeated three times (Shakibaie, Forootanfar, Golkari, Mohammadi-Khorsand, & Shakibaie, 2015; Vassena et al, 2014).…”

Section: Effect Of So Against S Aureus Biofilms Established On Milk mentioning

confidence: 99%

“…Stained biofilms were examined with a confocal laser scanning microscope (Leica, TCS SPS Ⅱ; Leica Microsystems CMS GmbH, Mannheim, Germany). For each sample, at least 10 random positions in two independent cultures were chosen for microscopic analysis (Kim, Lee, Kim, Baek, & Lee, 2015;Vassena et al, 2014).…”

Section: Confocal Laser Scanning Microscopy (Clsm) Studiesmentioning

confidence: 99%

The specific antibacterial effect of the Salvia oil nanoliposomes against Staphylococcus aureus biofilms on milk container

2016

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“…The analysis was carried out following a method previously developed for quantitative analysis of biofilm. 16 Biofilm was stained with a fluorescent stain (FilmTracer TM LIVE/DEAD® Biofilm Viability Kit; Thermo Fisher Scientific) containing a mixture of two dyes: SYTO® 9 (green stain) and propidium iodide (PI; red stain), 3 μl of each component in 1 ml of saline solution. SYTO® 9 generally labels all bacteria in a population, while PI penetrates only bacteria with damaged membranes.…”

Section: Confocal Laser Scanning Microscopy Analysismentioning

confidence: 99%

Antibiofilm activity of sandblasted and laser-modified titanium against microorganisms isolated from peri-implantitis lesions

Drago

Bortolin

Vecchi

et al. 2016

Journal of Chemotherapy

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Infections due to biofilm-producing microorganisms are one of the main causes for the failure of dental implants. Increasing efforts have been made in order to develop new strategies to prevent biofilm formation. In this study, the biofilm development on a newly designed laser-modified titanium implant surface was evaluated and compared to that on conventional sandblasted titanium used in implant dentistry. The amount of biofilm produced by Staphylococcus aureus, Pseudomonas aeruginosa and Porphyromonas gingivalis isolated from peri-implantitis was assessed by a semi-quantitative spectrophotometric method and by confocal laser scanning microscopy. Results showed a lower biofilm production on laser-modified surface compared to the sandblasted one. In particular, a significantly lower total volume of the biomass was observed on laser-modified surface, while no significant changes in live/dead bacteria percentages were noticed between materials. Modifying the topography of the conventional implant surface with laser ablation could represent a promising approach for inhibiting biofilm formation.

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Photodynamic antibacterial and antibiofilm activity of RLP068/Cl against Staphylococcus aureus and Pseudomonas aeruginosa forming biofilms on prosthetic material

Cited by 59 publications

References 29 publications

Antimicrobial photodynamic inactivation: a bright new technique to kill resistant microbes

Antimicrobial photodynamic inactivation: a bright new technique to kill resistant microbes

The specific antibacterial effect of the Salvia oil nanoliposomes against Staphylococcus aureus biofilms on milk container

Antibiofilm activity of sandblasted and laser-modified titanium against microorganisms isolated from peri-implantitis lesions

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