Photochemical Synthesis of the Bioconjugate Folic Acid-Gold Nanoparticles

Castillo, Jaime; Bertel, Linda; Páez‐Mozo, Edgar A.; Martı́nez, Fernando

doi:10.5772/57144

Cited by 10 publications

(9 citation statements)

References 26 publications

(33 reference statements)

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“…The TEM result of AuNPs was shown in Figure 2, which indicated that the AuNPs had a uniform size; the diameter was about 16 nm, as well as the particle shape was spherical; and the dispersion was even [22].…”

Section: Resultsmentioning

confidence: 99%

Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate

Meng

Zhao

Guo

et al. 2016

Nanomaterials and Nanotechnology

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A fluorescence resonance energy transfer (FRET)-based aptamer biosensor (aptasensor) for sensitive determination of adenosine triphosphate (ATP) was developed. The energy donor and acceptor were 5′-carboxyfluorescein (5′-FAM) and gold nanoparticles (AuNPs), respectively. AuNPs were linked to 3′-SH-DNA by Au-S bond. This aptasensor was obtained by the hybridization between the complementary DNA strands of the 5′-FAM and AuNPs. The detecting results indicated that the FRET increase efficiency (F r) of detecting system at 519 nm was linear when the concentration of ATP target was about 0.1-10 mmol/L. Moreover, the aptasensors showed a good stability in different buffer conditions and a low-detection limit (15.2 nmol/L) for ATP.

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Section: Resultsmentioning

confidence: 99%

Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate

Meng

Zhao

Guo

et al. 2016

Nanomaterials and Nanotechnology

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“…FA was obtained from Sigma-Aldrich Corp. All chemicals used in this study were of analytical grade. FA solutions were prepared as described by Castillo et al [17]. FA molecules were dissolved in water with an addition of 50 µL of NaOH (1 M), due to the poor solubility of FA.…”

Section: Preparation Of Fa Solutionsmentioning

confidence: 99%

Adsorption and Vibrational Study of Folic Acid on Gold Nanopillar Structures Using Surface-Enhanced Raman Scattering Spectroscopy

Castillo

Rindzevicius

Rozo

et al. 2015

Nanomaterials and Nanotechnology

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This paper presents a study of adsorption and vibrational features of folic acid, using surface-enhanced Raman scattering (SERS). A gold-capped silicon nanopillar (Au NP) with a height of 600 nm and a width of 120 nm was utilized to study the vibrational features of FA molecules adsorbed on the nanopillars within the high electromagnetic field areas. The adsorption behaviour of folic acid and the band assignment of the main vibrations together with the optimized geometry of folic acid and folic acid in the presence of a cluster of 10 gold atoms were assessed using the density functional theory (B3LYP(6-31G(d))) and the scalar relativistic effective core potential with a double-zeta basis set (LANL2DZ). The vibrations obtained from the solid-state folic acid and the folic acid on a gold cluster were in accordance with those observed experimentally. The analysis of the main vibrations indicated that the interaction of folic acid with the Au NP occurred primarily through the nitrogen atoms, from their pteridine ring. Finally, the obtained adsorption isotherm for folic acid was deduced from the analysis of the SERS spectra and it followed a negative cooperative binding model.

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“…Four P6CA/HAuCl 4 .3H 2 O molar ratios (0.44/1, 0.5/1, 0.56/1, and 0.62/1) were used to evaluate the effect of AuNP growth in the synthesis of P6CA-AuNP. These molar ratios were selected based on previous FA-AuNP synthesis works [18,25]. When UV-Vis spectra of products were analyzed, the characteristic SPA band of spherical AuNPs could be observed around 530 nm for 0.56/1 ratio (see Fig.…”

Section: Resultsmentioning

confidence: 99%

Laser photo-thermal therapy of epithelial carcinoma using pterin-6-carboxylic acid conjugated gold nanoparticles

Bertel

Méndez‐Sánchez

Ortega

2021

Photochem Photobiol Sci

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Gold nanoparticles functionalized with folic acid toward the internalization into cancer cells have received considerable attention recently. Folic acid is recognized by folate receptors, which are overexpressed in several cancer cells; it is limited in normal cells. In this work, pterin-6-carboxylic acid is proposed as an agonist of folic acid since the pterin-6-carboxylic acid structure has a pterin moiety, the same as folic acid that is recognized by the folate receptor. Here a simple photochemical synthesis of gold nanoparticles functionalized with pterin-6-carboxylic acid is studied. These conjugates were used to cause photothermal damage of HeLa cells irradiating with a diode laser of 808 nm. Pterin-6-carboxylic acid-conjugated gold nanoparticles caused the death of the cell after near-infrared irradiation, dose-dependently. These results indicate a possible internalization of AuNPs via folate receptor-mediated endocytosis due to the recognition or interaction between the folate receptors of HeLa cells and pterin, P6CA.

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Photochemical Synthesis of the Bioconjugate Folic Acid-Gold Nanoparticles

Cited by 10 publications

References 26 publications

Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate

Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate

Adsorption and Vibrational Study of Folic Acid on Gold Nanopillar Structures Using Surface-Enhanced Raman Scattering Spectroscopy

Laser photo-thermal therapy of epithelial carcinoma using pterin-6-carboxylic acid conjugated gold nanoparticles

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