Photochemical and phototoxic activity of berberine on murine fibroblast NIH-3T3 and Ehrlich ascites carcinoma cells

Jantová, Soňa; Letašiová, Silvia; Brezová, Vlasta; Čipák, Luboš; Lábaj, Juraj

doi:10.1016/j.jphotobiol.2006.07.001

Cited by 47 publications

(34 citation statements)

References 41 publications

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“…Higher concentrations of berberine resulted in a G2/M arrest, which is suggestive of a DNA damage/p53 activation response [47,48]. Although we did not directly assay DNA damage in this study, previous workers have presented evidence for berberine-induced DNA damage and p53 activation [11,38,46].…”

Section: Discussionmentioning

confidence: 56%

“…Previous studies in fact have shown that berberine inhibits NADH dehydrogenase and respiratory activity [23,37]. In addition, mitochondrial participation in berberine-induced apoptosis has been demonstrated in a number of studies [2,6,11,38]. Mitochondrially-relevant eVects of berberine exposure include changes in Bcl-2/Bax ratios, reactive oxygen species production, and a reduction in mitochondrial membrane potential [6,11].…”

Section: Discussionmentioning

confidence: 95%

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Different concentrations of berberine result in distinct cellular localization patterns and cell cycle effects in a melanoma cell line

Serafim

Oliveira

Sardão

et al. 2007

Cancer Chemother Pharmacol

117

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Purpose Natural products represent a rich reservoir of potential small molecule inhibitors exhibiting antiproliferative and tumoricidal properties. An example is the isoquinoline alkaloid berberine, which is found in plants such as goldenseal (Hydrastis canadensis). Studies have shown that berberine is able to trigger apoptosis in diVerent malignant cell lines, and can also lead to cell cycle arrest at sub-apoptotic doses. A particularly interesting feature of berberine is the fact that it is a Xuorescent molecule, and its uptake and distribution in cells can be studied by Xow cytometry and epiXuorescence microscopy. To test the relationships between berberine uptake, distribution and cellular eVect in melanoma cells, K1735-M2 mouse and WM793 human melanoma cells were treated with diVerent concentrations of berberine, and alterations in cell cycle progression, DNA synthesis, cell proliferation, and cell death measured. Methods Cell proliferation was measured by sulforhodamine B assays, cell death by Xow cytometry, berberine uptake and distribution by laser scanning confocal microscopy and Xow cytometry, cell cycle progression by Xow cytometry, and DNA synthesis, M-phase, and mitochondrial eVects by immunolabeling and epiXuorescence microscopy methods. Results In these melanoma cell lines, berberine at low doses (12.5-50 M) is concentrated in mitochondria and promotes G1 arrest. In contrast, higher doses (over 50 M) result in cytoplasmic and nuclear berberine accumulation, and G2 arrest. DNA synthesis is not markedly aVected by low doses of berberine, but 100 M is strongly inhibitory. Even at 100 M, berberine inhibits cell growth with relatively little induction of apoptosis. Conclusion Berberine displays multiphasic eVects in these malignant cell lines, which are correlated with the concentration and intracellular distribution of this alkaloid. These results help explain some of the conXicting information in the literature regarding the eVects of berberine, and suggest that its use in clinical development may be more as a cytostatic agent than a cytotoxic compound.

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 95%

Different concentrations of berberine result in distinct cellular localization patterns and cell cycle effects in a melanoma cell line

Serafim

Oliveira

Sardão

et al. 2007

Cancer Chemother Pharmacol

117

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“…Chelerythrine (10) was shown to be inhibitory against SQ-20B [10], NUGC [11], HepG2 [12], KB [13], LNCaP, and DU-145 [14] cell lines. Berberine (2) is cytotoxic to many tumor cell lines including hepatoma cells, leukemia cells [15] [16], and HT-29, Hep-2 (larynx tumor), MCF-2 (breast cancer), MKN (gastric cancer) [17], SNU-5 (gastric cancer) [18], B16 (murine melanoma) [19], and EAC [20] cell lines. In summary, previous anticarcinogenic studies of these alkaloids focused mainly on their cytotoxic activities in vitro.…”

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confidence: 99%

DNA Topoisomerase I Inhibitory Alkaloids from Corydalis saxicola

Cheng

Wang

Jiang

et al. 2008

Chemistry & Biodiversity

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Chemical studies of the Chinese herb Corydalis saxicola Bunting led to the isolation and identification of 14 alkaloids, 1-14. Seven of these compounds, 4-9 and 11, were obtained from this plant for the first time. Feruloylagmatine (7) is the first guanidine-type alkaloid to be identified in the family Papaveraceae and in dicotyledonous plants. All of the isolated compounds were assayed for inhibitory activity against human DNA topoisomerase I. A DNA cleavage assay demonstrated that these alkaloids specifically inhibit topoisomerase through stabilization of the enzyme-DNA complex. Among the isolated alkaloids, (-)-pallidine (8) and (-)-scoulerine (11) showed strong inhibitory activities toward topoisomerase I that were comparable to camptothecin, a typical topoisomerase I inhibitor. A preliminary structure-activity relationship study suggested that the quaternary ammonium ion might play an important role in topoisomerase I inhibition by the isoquinoline alkaloids. These data indicated that DNA topoisomerase I inhibition represents probably one of the anticarcinogenic mechanisms of C. saxicola.

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“…Plusieurs travaux ont noté plutôt des effets immunostimulants [2,6,32], apoptotiques [9,11,19,22] et anticancéreux [8,12,24,25,30,39] de la berberine. Cependant, aucun travail ne s'est intéressé à évaluer l'effet suppresseur des composés de l'extrait aqueux et celui de la fraction protéique de Berberis hispanica sur le pourcentage des sous-populations leucocytaires.…”

Section: Discussionunclassified

Antidepressive, antinociceptive and immunomodulatory effects of aqueous and proteic extracts of Berberishispanica Boiss. & Reut. from Morocco

et al. 2011

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Photochemical and phototoxic activity of berberine on murine fibroblast NIH-3T3 and Ehrlich ascites carcinoma cells

Cited by 47 publications

References 41 publications

Different concentrations of berberine result in distinct cellular localization patterns and cell cycle effects in a melanoma cell line

Different concentrations of berberine result in distinct cellular localization patterns and cell cycle effects in a melanoma cell line

DNA Topoisomerase I Inhibitory Alkaloids from Corydalis saxicola

Antidepressive, antinociceptive and immunomodulatory effects of aqueous and proteic extracts of Berberishispanica Boiss. & Reut. from Morocco

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