2019
DOI: 10.1021/acs.analchem.8b04434
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Abstract: Gold nanoparticles (AuNPs) have shown great promise as a universal platform for biosensing and are often functionalized with a densely packed DNA for intracellular detection. While DNA–AuNP conjugates, such as nanoflares, have been used for single and multiple mRNA molecules detection in living cells, the target recognition reaction is triggered once they enter into cells, making it impossible to control the initial reaction at the desired time. To solve this problem, we have designed photoactivated (PA) nanof… Show more

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Cited by 39 publications
(34 citation statements)
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“…Besides, it cannot be denied that SmartFlare TM probe detection is not indicative of the real localization of single mRNA molecules. Nevertheless, those NanoFlare probes have paved the way to inspire a novel theranostic wave arising some new sticky-flares for in situ monitoring of human telomerase RNA[ 37 ], adopting photoactivation to detect mRNA in specific cells[ 38 ].…”
Section: Discussionmentioning
confidence: 99%
“…Besides, it cannot be denied that SmartFlare TM probe detection is not indicative of the real localization of single mRNA molecules. Nevertheless, those NanoFlare probes have paved the way to inspire a novel theranostic wave arising some new sticky-flares for in situ monitoring of human telomerase RNA[ 37 ], adopting photoactivation to detect mRNA in specific cells[ 38 ].…”
Section: Discussionmentioning
confidence: 99%
“…Significant advances in nanotechnology have provided many functional nanomaterials to develop biosensors with improved analytical performance. , Particularly, the gold nanoparticle (AuNP)-based spherical nucleic acids (SNAs) have been used widely in the field of biosensing because of their good robustness, high sensitivity, and easy readout by the naked eyes. Recent progress in the synthesis methodology has achieved the construction of SNAs in time- and cost-effective ways, greatly boosting its potential applications in clinical diagnostics. Inspired by these advances in synthetic biology and nanotechnology, we seek to establish a sensitive method for the colorimetric analysis of protease biomarkers by the modular combination of proteolysis-responsive transcription with SNAs. As shown in Scheme , each proteolytic event mediated by the target protease results in the generation of an active RNAP, which subsequently produces multiple RNAs with a programmable sequence by in vitro transcription.…”
Section: Introductionmentioning
confidence: 99%
“…3 This new design of oligonucleotide -coated NPs, also termed spherical nucleic acids (SNAs) have subsequently emerged as live cell probes for the detection of targets including RNA, molecules and ions. [4][5][6][7][8][9][10][11][12] The extensive use of SNAs within the biomedical field is primarily due to their high specificity, binding of complementary oligonucleotide strands and improved resistance towards DNA enzymatic degradation as well as the enhanced uptake of SNAs by multiple types of cells. 13,14 Seferos et al were the first to show how SNAs could be hybridised to shorter fluorophore bearing oligonucleotides for the targeted detection of mRNA.…”
Section: Introductionmentioning
confidence: 99%
“…By illuminating specific cells of interest, mRNA detection was visualized only in these specific cells, while neighboring cells remained fluorescently dormant. 6 The design of SNAs has been further exploited for the detection of other targets including miRNAs and, recently, for the delivery of oligonucleotides or peptides to activate the immune system. 5,[23][24][25][26][27][28] Zhai et al reported the detection of microRNA -1246, a breast cancer biomarker, in human plasma exosomes, whereas, Zhao et al used the same design for the direct detection of microRNA -375.…”
Section: Introductionmentioning
confidence: 99%
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