Photoacoustic imaging of acupuncture effect in small animals

Li, Tingting; Xu, Xueliang; Chen, Bingzhang; Rong, Jian; Jiang, Huabei

doi:10.1364/boe.6.000433

Cited by 8 publications

(11 citation statements)

References 18 publications

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“…Because the HbT in tissue has a very high light absorption coefficient at 532nm compared with other absorbers, we assumed that the distribution of the RLA represents the distribution of HbT concentration. However, we have demonstrated that this experimental setup can be better applied to acupuncture study in our previous work [26].…”

Section: Methodsmentioning

confidence: 94%

Photoacoustic imaging of cerebral hypoperfusion during acupuncture

Chen

Yang

et al. 2015

Biomed. Opt. Express

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Using acupuncture to treat cerebral hypoperfusion is a hot topic. However, there is a lack of effective tools to clarify the therapeutic effect of acupuncture on cerebral hypoperfusion. Here, we show in a mouse model of cerebral hypoperfusion that photoacoustic tomography (PAT) can noninvasively image cerebral vasculature and track total hemoglobin (HbT) concentration changes in cerebral hypoperfusion with acupuncture stimulation on the YangLingQuan (GB34) point. We measured the changes of HbT concentration and found that the HbT concentration in hypoperfusion regions was clearly lower than that in the control regions when the acupuncture was absent; however, it was significantly increased when the acupuncture was implemented on the GB34 point. We also observed the increase of vessel size and the generation of new vessels in cerebral hypoperfusion during acupuncture. Laser speckle imaging (LSI) was employed to validate some of the PAT findings. #241512Received 22

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Section: Methodsmentioning

confidence: 94%

Photoacoustic imaging of cerebral hypoperfusion during acupuncture

Chen

Yang

et al. 2015

Biomed. Opt. Express

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“…The KI1 acupoint is located in the middle of the hind paw. The pubic symphysis was obliquely stabbed at point CV4 at the mouse abdomen median line, 10 mm below the navel [22,42,43] (Figure 6B). AG mice received acupuncture at both sides of ST36 and CV4 with 13-mm needles.…”

Section: Acupuncture Manipulationmentioning

confidence: 99%

Acupuncture on ST36, CV4 and KI1 Suppresses the Progression of Methionine- and Choline-Deficient Diet-Induced Nonalcoholic Fatty Liver Disease in Mice

et al. 2019

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Nonalcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases worldwide, and its treatment remain a constant challenge. A number of clinical trials have shown that acupuncture treatment has beneficial effects for patients with NAFLD, but the molecular mechanisms underlying its action are still largely unknown. In this study, we established a mouse model of NAFLD by administering a methionine- and choline-deficient (MCD) diet and selected three acupoints (ST36, CV4, and KI1) or nonacupoints (sham) for needling. We then investigated the effects of acupuncture treatment on the progression of NAFLD and the underlying mechanisms. After two weeks of acupuncture treatment, the liver in the needling-nonapcupoint group (NG) mice appeared pale and yellowish in color, while that in the needling-acupoint group (AG) showed a bright red color. Histologically, fewer lipid droplets and inflammatory foci were observed in the AG liver than in the NG liver. Furthermore, the expression of proinflammatory signaling factors was significantly downregulated in the AG liver. A lipid analysis showed that the levels of triglyceride (TG) and free fatty acid (FFA) were lower in the AG liver than in the NG liver, with an altered expression of lipid metabolism-related factors as well. Moreover, the numbers of 8-hydroxy-2′-deoxyguanosine (8-OHdG)-positive hepatocytes and levels of hepatic thiobarbituric acid reactive substances (TBARS) were significantly lower in AG mice than in NG mice. In line with these results, a higher expressions of antioxidant factors was found in the AG liver than in the NG liver. Our results indicate that acupuncture repressed the progression of NAFLD by inhibiting inflammatory reactions, reducing oxidative stress, and promoting lipid metabolism of hepatocytes, suggesting that this approach might be an important complementary treatment for NAFLD.

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“…Many studies have shown that there is a relationship between acupoints and functional brain areas. [27][28][29][30][31][32][33][34][35][36][37][38][39][40][41] The ST36 point is located laterally and inferiorly to the knee joint, 3.5 mm below the ca-pitula¯bula. There are many acupoints including the ST36 point in the circulation of Foot-Yangming stomach meridian.…”

Section: Acupoint Selectionmentioning

confidence: 99%

“…[27][28][29][30] An extensive body of research has demonstrated that the regulation e®ect of acupuncture and moxibustion at the acupoints on humans and animals is closely related to the e®ect and adjusting function of the stimulation on the nervous system. [27][28][29][30][31][32][33][34][35][36][37][38][39][40][41] Acupuncture at the peripheral nervous system can exert a di®erent in°uence on the central nervous system including the brain stem, hypothalamus, basal ganglia and cerebral cortex. 39 Previous research in our laboratory has shown that in acupuncture, the Yongquan (KI1) and the Yanglingquan (GB34) acupoints can noninvasively increase the hemodynamics, including the cerebral blood volume (CBV) and total hemoglobin (HbT) in the in vivo mouse brain.…”

Section: Introductionmentioning

confidence: 99%

“…39 Previous research in our laboratory has shown that in acupuncture, the Yongquan (KI1) and the Yanglingquan (GB34) acupoints can noninvasively increase the hemodynamics, including the cerebral blood volume (CBV) and total hemoglobin (HbT) in the in vivo mouse brain. [31][32][33][34] Here, we considered poly(ethyleneglycol)-coated (PEGylated) gold nanorods (PEG-GNRs) as the photoacoustic contrast agent and considered acupuncture as a physical auxiliary method to adjust the hemodynamics of the mouse brain. In the study, we developed a method based on combined acupuncture and GNRs (as contrast agents), in which GNRs were injected intravenously and the concentration of hemoglobin in the cerebral cortex of the mice was increased by acupuncture at the Zusanli (ST36) acupoint.…”

Section: Introductionmentioning

confidence: 99%

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Photoacoustic molecular imaging using combined acupuncture and gold nanorods as a composite contrast agent

Zhang

Rong

et al. 2019

J. Innov. Opt. Health Sci.

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In this study, we developed a novel photoacoustic imaging technique based on poly (ethyleneglycol)-coated (PEGylated) gold nanorods (PEG-GNRs) (as the contrast agent) combined with traditional Chinese medicine (TCM) acupuncture (as the auxiliary method) for quantitatively monitoring contrast enhancement in the vasculature of a mouse brain in vivo. This study takes advantage of the strong near-infrared absorption (peak at [Formula: see text][Formula: see text]nm) of GNRs and the ability to adjust the hemodynamics of acupuncture. Experimental results show that photoacoustic tomography (PAT) successfully reveals the optical absorption variation of the vasculature of the mouse brain in response to intravenous administration of GNRs and acupuncture at the Zusanli acupoint (ST36) both individually and combined. The quantitative measurement of contrast enhancement indicates that the composite contrast agents (integration of acupuncture and GNRs) would greatly enhance the photoacoustic imaging contrast. The quantitative results also have the potential to estimate the local concentration of GNRs and even the real-time effects of acupuncture.

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Photoacoustic imaging of acupuncture effect in small animals

Cited by 8 publications

References 18 publications

Photoacoustic imaging of cerebral hypoperfusion during acupuncture

Photoacoustic imaging of cerebral hypoperfusion during acupuncture

Acupuncture on ST36, CV4 and KI1 Suppresses the Progression of Methionine- and Choline-Deficient Diet-Induced Nonalcoholic Fatty Liver Disease in Mice

Photoacoustic molecular imaging using combined acupuncture and gold nanorods as a composite contrast agent

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