Cell Reports volume 3, issue 4, P1036-1043 2013 DOI: 10.1016/j.celrep.2013.03.017 View full text
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Alexandra Cribier, Benjamin Descours, Ana Luiza Chaves Valadão, Nadine Laguette, Monsef Benkirane

Abstract: SAMHD1 restricts HIV-1 replication in myeloid and quiescent CD4(+) T cells. Here, we show that SAMHD1 restriction activity is regulated by phosphorylation. SAMHD1 interacts with cyclin A2/cdk1 only in cycling cells. Cyclin A2/CDK1 phosphorylates SAMHD1 at the Threonine 592 residue both in vitro and in vivo. Phosphorylation of SAMHD1 Thr592 correlates with loss of its ability to restrict HIV-1. Indeed, while PMA treatment of proliferating THP1 cells results in reduced Thr592 phosphorylation, activation of resti…

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