Cell Reports volume 5, issue 4, P1070-1081 2013 DOI: 10.1016/j.celrep.2013.10.017 View full text
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Kristina M. Herbert, Genaro Pimienta, Suzanne J. DeGregorio, Andrei Alexandrov, Joan A. Steitz

Abstract: SUMMARY During miRNA biogenesis, the microprocessor complex (MC), which is composed minimally of Drosha, an RNase III enzyme, and DGCR8, a double-stranded RNA-binding protein, cleaves the primary miRNA (pri-miRNA) in order to release the pre-miRNA stem-loop structure. Using phosphoproteomics, we mapped 23 phosphorylation sites on full-length human DGCR8 expressed in insect or mammalian cells. DGCR8 can be phosphorylated by mitogenic ERK/MAPK, indicating that DGCR8 phosphorylation may respond to and integrate …

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