Phospholipids fromBombycis corpus and their neurotrophic effects

Kwon, Hak Cheol; Jung, I-Yeon; Cho, Se Yeon; Cho, Ock Ryun; Min, Yang; Lee, Sung Ok; Hur, Jin Young; Kim, Sun Yeou; Yang, Jong Beom; Lee, Kang Ro

doi:10.1007/bf02976865

Cited by 15 publications

(8 citation statements)

References 13 publications

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“…11,12) In the present study, we found that BLP-conditioned media of C6 and primary astrocyte cells caused the induction of PC 12 neurite outgrowth. Since NGF is known to be an endogenous molecule that contributes to the differentiation of PC12 cells, 13) we hypothesized that BLP might enhance NGF secretion and synthesis in astrocyte cells.…”

Section: Discussionsupporting

confidence: 51%

Induction of Nerve Growth Factor by Butanol Fraction of Liriope platyphylla in C6 and Primary Astrocyte Cells

Hur

Lee

Kim

et al. 2004

Biological & Pharmaceutical Bulletin

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Astrocytes play an essential role in maintaining the physiological function of neurons. In particular, neurotrophic factors (NFs) produced and released by astrocytes contribute to the differentiation and survival of neuronal cells.1) Among the NFs, nerve growth factor (NGF), first identified to be a member of the NF family, 2) has been reported to have therapeutic potency in the treatment of neurodegenerative diseases such as Alzheimer's disease and cerebrovascular dementia. 3)However, since its large molecular weight and susceptibility to endogenous peptidase render NGF unable to penetrate the blood-brain-barrier, it can only be used for medical application when directly injected into the brain. 4) Therefore, much attention has been paid to the agents that either enhance the action of NGF or increase the NGF synthesis. Although the mechanism of NGF expression is not fully understood, it has been reported that the expression of NGF is regulated through a multiple signaling pathway. Phorbol ester increased NGF expression in astrocytes, suggesting the involvement of protein kinase C (PKC). 5)Liriope platyphylla (LP) is a medicinal herb that has been used for treating cough and sputum in Korea. With regards to the pharmacological effect of LP, antibacterial and anticancer effects have been reported. 6,7) However, the neuroprotective effect of LP remains to be examined. In this study, we investigated whether the butanol fraction of LP (BLP)-conditioned media of astrocytes triggered neurite outgrowth in PC 12 cells that was differentiated into neuron-like cells. 8) In addition, the effects of BLP on astroglial NGF secretion and synthesis were also examined. MATERIALS AND METHODS MaterialsDulbecco's modified Eagle medium (DMEM), RPMI 1640, fetal bovine serum (FBS), horse serum (HS), penicillin-streptomycin (PS), and NGF were purchased from Gibco-BRL (Gaithersburg, MD, U.S.A.). Antibody against mouse NGF was obtained from Chemicon (Temecula, CA, U.S.A.).Preparation of Butanol Fraction of Liriope platyphylla LP was obtained from Milyang province of South Korea and identified by Prof. H. Kim of the Herbal Pharmacology Department of Kyung Hee University, where a voucher specimen (HP-SY-01) is deposited at the herbarium of the Graduate School of East-West Medical Science. The dried tubers of LP (6 kg) were extracted with 85% MeOH three times under an ultrasonic apparatus. The total extract concentrated with a rotary evaporator (572 g) was suspended in distilled water and partitioned with EtOAc (30 g) and BuOH (60 g), successively. No detailed chemical investigation appears to have been conducted on this plant. However, recently, the steroidal saponin, Spicatoside A, was isolated and characterized. BLP was standardized by determining the contents of spicatoside A, a major saponin.9) The content of spicatoside A is 0.4% (data not shown).Cell Culture C6, a rat astrocyte cell line, and PC 12 cells were obtained from the American Type Culture Collection (Rockville, MD. U.S.A.). C6 glioma cells were cultured in DMEM supplemented...

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Section: Discussionsupporting

confidence: 51%

Induction of Nerve Growth Factor by Butanol Fraction of Liriope platyphylla in C6 and Primary Astrocyte Cells

Hur

Lee

Kim

et al. 2004

Biological & Pharmaceutical Bulletin

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“…Five fatty acids were isolated from B. batryticatus : meso-erythritol ( 3 ), citric acid ( 4 ), decanoic acid ( 5 ), stearic acid ( 6 ) and palmitic acid ( 7 ) [ 43 , 44 , 45 ]. Seven derivatives of fatty acids in B. batryticatus were identified: (4 E ,2 S ,3 R )-2- N -octadecanoyl-4-tetradecasphingenine ( 8 ), (4 E ,6 E ,2 S ,3 R )-2- N -eicosanoyl-4,6-tetradecasphingadienine ( 9 ), (4 E ,2 S ,3 R )-2- N -eicosanoyl-4-tetradecasphingenine ( 10 ), (4 E ,6 E ,2 S ,3 R )-2- N -docosanoyl-4,6-tetradecasphingadienine ( 11 ), 1,2-di- O -hexadecanoyl-sn-glycero-3-phosphorylcholin ( 12 ), 1- O -(9 Z -octadecenoyl)-2- O -(8 Z ,11 Z -octadecadienoyl)-sn-glycero-3-phosphorylcholin ( 13 ) and 1,2-di- O -9 Z -octadecenoyl-sn-glycero-3-phosphorylcholin ( 14 ) [ 46 , 47 ].…”

Section: Chemistrymentioning

confidence: 99%

“…In 1996, four nucleotides were detected in B. batryticatus by Li et al (1996) through HPLC method, including uracil ( 19 ), uridine ( 20 ), hypoxanthine ( 21 ) and xanthine ( 22 ), and among them, uracil content was highest [ 47 ]. Later, in 2003, uracil ( 19 ), cytidine ( 23 ) and adenine ( 24 ) were isolated from B. batryticatus by Kwon et al (2003b) [ 49 ].…”

Section: Chemistrymentioning

confidence: 99%

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Traditional Uses, Origins, Chemistry and Pharmacology of Bombyx batryticatus: A Review

Zhang

Wang

et al. 2017

Molecules

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Bombyx batryticatus (B. batryticatus), a well-known traditional animal Chinese medicine, has been commonly used in China for thousands of years. The present paper reviewed advances in traditional uses, origin, chemical constituents, pharmacology and toxicity studies of B. batryticatus. The aim of the paper is to provide more comprehensive references for modern B. batryticatus study and application. In Traditional Chinese Medicine (TCM) culture, drugs containing B. batryticatus have been used to treat convulsions, headaches, skin prurigo, scrofula, tonsillitis and fever. Many studies indicate B. batryticatus contains various compounds, including protein and peptides, fatty acids, flavonoids, nucleosides, steroids, coumarin, polysaccharide and others. Numerous investigations also have shown that extracts and compounds from B. batryticatus exert a wide spectrum of pharmacological effects both in vivo and in vitro, including effects on the nervous system, anticoagulant effects, antitumor effects, antibacterial and antifungal effects, antioxidant effects, hypoglycemic effects, as well as other effects. However, further studies should be undertaken to investigate bioactive compounds (especially proteins and peptides), toxic constituents, using forms and the quality evaluation and control of B. batryticatus. Furthermore, it will be interesting to study the mechanism of biological activities and structure-function relationships of bioactive constituents in B. batryticatus.

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“…Further, BB contains different proteins, peptides, fatty acids, flavonoids, nucleosides, steroids, coumarin, and polysaccharides [7]. Moreover, BB has recently been shown to exhibit neuroprotective and anti-necrotic effects in pyramidal neurons, neurotrophic (upregulation of nerve growth factor) and anti-oxidative (upregulation of superoxide dismutase, SOD) effects in astrocytes, and antiapoptotic effects (inhibition of lipid peroxidation) in hippocampal neurons [7][8][9]. Recently, Hu et al reported that BB exerts neuroprotective, anti-oxidative, and anti-apoptotic effects against pentylenetetrazole-or H 2 O 2 -induced epileptic neurotoxicity via the PI3K/Akt signaling pathways [10].…”

Section: Introductionmentioning

confidence: 99%

Batryticatus Bombyx Protects Dopaminergic Neurons against MPTP-Induced Neurotoxicity by Inhibiting Oxidative Damage

et al. 2019

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Oxidative stress plays an important role in the degeneration of dopaminergic neurons in Parkinson’s disease (PD). Altered redox homeostasis in neurons interferes with several biological processes, ultimately leading to neuronal death. Oxidative damage has been identified as one of the principal mechanisms underlying the progression of PD. Several studies highlight the key role of superoxide radicals in inducing neuronal toxicity. Batryticatus Bombyx (BB), the dried larva of Bombyx mori L. infected by Beauveria bassiana (Bals.) Vuill., has been used in traditional medicine for its various pharmacological effects. In the present study, BB showed a beneficial effect on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced neurotoxicity by directly targeting dopaminergic neurons. Treatment with BB improved behavioral impairments, protected dopaminergic neurons, and maintained dopamine levels in PD mouse models. Here, we investigated the protective effects of BB on MPTP-induced PD in mice and explored the underlying mechanisms of action, focusing on oxidative signaling. In MPTP-induced PD, BB promoted recovery from impaired movement, prevented dopamine depletion, and protected against dopaminergic neuronal degradation in the substantia nigra pars compacta (SNpc) or the striatum (ST). Moreover, BB upregulated mediators of antioxidative response such as superoxidase dismutase (SOD), catalase (CAT), glutathione (GSH), Heme oxygenase 1 (HO-1), and NAD(P)H (nicotinamide adenine dinucleotide phosphate) dehydrogenase (NQO1). Thus, treatment with BB reduced the oxidative stress, improved behavioral impairments, and protected against dopamine depletion in MPTP-induced toxicity.

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Phospholipids fromBombycis corpus and their neurotrophic effects

Cited by 15 publications

References 13 publications

Induction of Nerve Growth Factor by Butanol Fraction of Liriope platyphylla in C6 and Primary Astrocyte Cells

Induction of Nerve Growth Factor by Butanol Fraction of Liriope platyphylla in C6 and Primary Astrocyte Cells

Traditional Uses, Origins, Chemistry and Pharmacology of Bombyx batryticatus: A Review

Batryticatus Bombyx Protects Dopaminergic Neurons against MPTP-Induced Neurotoxicity by Inhibiting Oxidative Damage

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