Phospholipids and Nuclear Rna

Albi, Elisabetta; Micheli, Maria Rita; Magni, Michele

doi:10.1006/cbir.1996.0051

Cited by 38 publications

(28 citation statements)

References 23 publications

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“…The simultaneous treatment with phospholipase A2 and RNA colloidal gold using particles of different sizes revealed that nuclear PLs and RNA have a similar location inside the nucleus. 23 The relation between RNA and PLs was confirmed by the fact that digestion of isolated hepatocyte nuclei with DNase did not change the PL content whereas a decrease of 40% was observed after DNase and RNase treatment 24 ( Figure 3). The loss was not equal for each PL.…”

Section: Sphingomyelin Presence and Localizationmentioning

confidence: 78%

“…25 The result expressed as mg mg À1 of protein confirmed the strong decrease in SM after RNAse digestion. 24 When the hepatocyte nuclei were treated with Triton-X-100 to remove the external envelope and digested with DNAse and RNAse the remaining material was mostly constituted by protein, but a small amount of RNAse-resistant RNA remained together with PLs. Chromatographic analysis showed that only two PLs, SM and phosphatidylcholine (PC) were present 26 ( Figure 4).…”

Section: Sphingomyelin Presence and Localizationmentioning

confidence: 99%

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Chromatin‐associated sphingomyelin: metabolism in relation to cell function

Albi¹,

Magni²

2003

Cell Biochemistry & Function

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After the first histochemical demonstration by Chayen and Gahan of the presence of phospholipids and especially of sphingomyelin in chromatin, this became the object of long debate and of contradictory results. The general conclusion was that the presence of phospholipids may due to contamination during the isolation of chromatin. More recently the existence of a phospholipid chromatin fraction was confirmed by demonstrating that isolated hepatocyte nuclei, labelled by saturated and unsaturated radioiodination method, showed the presence of radioactivity only in the membrane and not in the isolated chromatin. The phospholipid composition showed an enrichment in sphingomyelin which increased during hepatocyte maturation or erythroleukemic cell differentiation induced by DMSO. A decrease in sphingomyelin was observed at the beginning of the Sphase in regenerating liver or in cultured proliferating cells. These changes were due to the presence of sphingomyelinase and sphingomyelin synthase in the chromatin, the activity of which paralleled the variation in sphingomyelin content. The sphingomyelin was co-localized with RNA as shown by biochemical and electron microscopy methods. Using bromo-uridine it was demonstrated that labelled RNA and sphingomyelin were present in actively transcribing nuclear regions. Isolated nuclear complexes after DNase and RNase digestion contained not only protein, but also RNA and sphingomyelin. After hydrolysis of sphingomyelin the RNAse-resistant RNA becomes RNAse sensitive. It can therefore be concluded that sphingomyelin and the related enzymes are present in the chromatin; sphingomyelin may have a role in RNA transcription protecting RNA by RNAse digestion before its transfer to the cytoplasm.

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Section: Sphingomyelin Presence and Localizationmentioning

confidence: 78%

Section: Sphingomyelin Presence and Localizationmentioning

confidence: 99%

Chromatin‐associated sphingomyelin: metabolism in relation to cell function

Albi¹,

Magni²

2003

Cell Biochemistry & Function

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“…Lipids are present in many cellular structures containing DNA and proteins, for instance, in nuclear matrix [12], nucleoid [13] and chromatin [14][15][16][17][18][19][20]. Literature provides more data on the presence of lipids in chromatin as compared to those in nuclear matrix and nucleoid.…”

Section: Lipids Of Chromatinmentioning

confidence: 99%

“…The relation of these lipids to transcription and replication processes could be interpreted by their close associations with DMC (nuclear pores and transcription factories). It has been demonstrated that in hepatocyte nuclei the chromatin phospholipid fraction is localized near the RNA in decondensed chromatin [18]. The necessity of developing new method of isolation of lipids tightly bound to chromatin (DMC) without using organic solvent and detergent is still relevant for these authors.…”

Section: Lipids Of Chromatinmentioning

confidence: 99%

DNA-Lipids-Me2+ Complexes Structure and their Possible Functions in a Cell

Vv¹

2016

J Chem Biol Ther

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Study of lipid-nucleic acids interactions have fifty years of history and were originally aimed at studying of DNAmembrane complexes (DMC). On the basis of own and literary data we had been proposed DMC model and their participation in the formation of structures nucleoid and nuclear envelope with pores.Understanding of nuclear pores role not only as channels between nucleus and cytoplasm but also as basic regulator of gene expression will allow using this knowledge in nanobiotechnology and medicine, in particular in gene therapy. In gene therapy the basic method of genes transfer in cells are complexes of DNA with which are strongly toxic for man. Zwitterionic lipids compose to 50 weight % of membranes and besides participation in DMC formation can really substitute cationic surfactants as carrier of DNA in cells.

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“…The occurrence of phospholipids in nuclei has been documented by biochemical and ultracytochemical procedures [2,3]. The total phospholipid content of nuclei is reported as 3% by weight compared with 75% for protein and 22% for DNA.…”

Section: Introductionmentioning

confidence: 99%

Signaling and interplay mediated by phospholipases A2, C, and D in LA-N-1 cell nuclei

Farooqui

Horrocks

2005

Reprod. Nutr. Dev.

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-Phospholipids are integral components of the nuclear membranes and intranuclear domains. Alterations in phospholipid metabolism occur during cellular differentiation, proliferation, and apoptosis, but the molecular mechanism involved in the above processes remains unknown. We propose that the coordinated expression of different genes responsible for the expression of transcription factors, neurotrophins, and cytokines, along with lipid mediators generated by the action of phospholipases A 2 , C, and D (PLA 2 , PLC, and PLD), play a very important role in differentiation, proliferation, and apoptosis. The purpose of this minireview is to discuss recent developments in PLA 2 , PLC, and PLD-mediated signaling in the nucleus of LA-N-1 neuroblastoma cell cultures. In brain tissue, arachidonic acid is mainly released by the action of PLA 2 and phospholipase C/diacylglycerol lipase (PLC/DAG-lipase) pathways. We have used LA-N-1 cell cultures to study activities of PLA 2 , C, and D during retinoic acid (RA)-mediated differentiation. The treatment of LA-N-1 cells with RA produces an increase in PLA 2 activity in the nuclear fraction. This increase in PLA 2 activity can be prevented with BMS493, a pan retinoic acid receptor antagonist, suggesting that RA-induced stimulation of PLA 2 activity is a RA receptor-mediated process. The treatment of LA-N-1 cells with 12-O-tetradecanoyl-phorbol-13 acetate (TPA) and RA increases diacylglycerol (DAG) levels indicating the stimulation of PLC activity. This stimulation is blocked by D609, tricyclodecan-9-yl potassium xanthate, a competitive PtdCho-specific PLC inhibitor. LA-N-1 cells also contain DAG-and monoacylglycerol (MAG) lipase activities. Two isoforms of PLD, oleate-dependent and TPA-dependent, are also present in LA-N-1 cell homogenates. RA stimulates the oleate-dependent isoform of PLD, whereas RA does not stimulate the TPA-dependent isoform. Our studies have indicated that lipid mediators generated by the action of PLA 2 , PLC, and PLD on nuclear phospholipids markedly affect neuritic outgrowth and neurotransmitter release in cells of neuronal and glial origin. We propose that RA receptors coupled with PLA 2 , PLC, and PLD activities in the nucleus may play an important role in the redistribution of arachidonic acid and its metabolites and DAG in nuclear and nonnuclear neuronal membranes during differentiation and growth suppression. nucleus / phospholipase A 2 / phospholipase C / phospholipase D / arachidonic acid / eicosanoids / peroxisome proliferators activated receptor

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Phospholipids and Nuclear Rna

Cited by 38 publications

References 23 publications

Chromatin‐associated sphingomyelin: metabolism in relation to cell function

Chromatin‐associated sphingomyelin: metabolism in relation to cell function

DNA-Lipids-Me2+ Complexes Structure and their Possible Functions in a Cell

Signaling and interplay mediated by phospholipases A2, C, and D in LA-N-1 cell nuclei

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