Phosphoglycerate Kinase B from Ram Testis

Stewart, Andrew; Scopes, Robert K.

doi:10.1111/j.1432-1033.1978.tb12215.x

Cited by 13 publications

(1 citation statement)

References 33 publications

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“…And so, although we cannot see any conclusive difference between bovine liver and muscle enzyme by this technique, neither are there many differences between the maps of bovine and rabbit muscle. Nevertheless, the genetically distinct isoenzymes phosphoglycerate kinase-A and phosphoglycerate kinase-B of sheep do have a few significant differences in peptide mapping (Stewart & Scopes, 1978). We feel that a genetically distinct isoenzyme present in liver for gluconeogenesis would have evolved at least by early vertebrate development, and would have diverged from phosphoglycerate kinase-A sufficiently to be detected by peptide mapping.…”

Section: Kinetic Measurementsmentioning

confidence: 89%

Purification of 3-phosphoglycerate kinase from diverse sources by affinity elution chromatography

Fifis¹,

Scopes²

1978

Biochemical Journal

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1. Affinity elution chromatography was used to purify phosphoglycerate kinase from a variety of sources. The choice of buffer pH for the chromatography was made according to the relative electrophoretic mobility of the enzyme from the species concerned. 2. Outlines of the methods used to isolate the enzyme from over 20 sources are presented. The enzyme was purified from the muscle tissue of a variety of mammals, fish and birds, from liver of several animals, from yeast, Escherichia coli, and plant leaves. The more acidic varieties of the enzymes were purified by conventional gradient elution from ion-exchangers as affinity elution procedures were not applicable. 3. The structural and kinetic parameters investigated show that phosphoglycerate kinase is evolutionarily a highly conservative enzyme; there were few differences in properties regardless of source or function (glycolytic, gluconeogenic or photosynthetic). 4. A detailed comparison of the enzyme preparations purified from bovine muscle and bovine liver failed to detect any significant differences between them; the evidence indicates that they are genetically identical.

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Section: Kinetic Measurementsmentioning

confidence: 89%

Purification of 3-phosphoglycerate kinase from diverse sources by affinity elution chromatography

Fifis¹,

Scopes²

1978

Biochemical Journal

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Developmental aspects of X chromosome inactivation in eutherian and metatherian mammals

VandeBerg

1983

J. Exp. Zool.

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The single active X principle has served for two decades as a focal point for research on the cyclic activation and inactivation of gene loci. Differences in X chromosome inactivation patterns of eutherian and marsupial mammals provide probes for investigating the mechanisms of the X inactivation process. In eutherian mammals, the X chromosome is inactivated early in meiotic prophase in males and remains inactive throughout the rest of spermatogenesis. During meiosis in females, the inactive X chromosome is activated so that both X chromosomes are active in oocytes. During the early cleavage divisions of female embryos, the paternally derived X is activated. It and the maternally derived X remain active until differentiation begins in early embryogenesis. At that time, the paternally derived X is inactivated in cells that give rise to extraembryonic membranes, whereas a random process determines which X chromosome is inactivated in cells that give rise to the embryo itself. Although less is known about developmental aspects of X inactivation in female marsupials, it is clear that the paternal X is preferentially inactive in postembryonic somatic cells. Furthermore, the paternal X is partially active at some loci in some cell types, indicating that it is not regulated as a single unit. The successful adaptation of a small (80-150 g), fecund marsupial to simple laboratory conditions now enables extensive experimentation on the large number of marsupials at various developmental stages. This capability, coupled with the application of newly developed cellular and molecular techniques to questions about X chromosome inactivation, shows great promise for advancing our understanding of the mechanisms that control the cyclic behavior of X chromosome activity.

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