Phosphate-Solubilizing Bacteria Nullify the Antagonistic Effect of Soil Calcification on Bioavailability of Phosphorus in Alkaline Soils

Adnan, Muhammad; Shah, Zahir; Fahad, Shah; Arif, Muhamamd; Alam, Mukhtar; Khan, Imtiaz Ali; Mian, Ishaq Ahmad; Basir, Abdul; Ullah, Hidayat; Arshad, Muhammad; Rahman, Inayat-Ur; Saud, Shah; Ihsan, Muhammad Zahid; Jamal, Yousaf; Amanullah, .; Hammad, Hafiz Mohkum; Nasim, Wajid

doi:10.1038/s41598-017-16537-5

Cited by 105 publications

(70 citation statements)

References 69 publications

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“…It could be revealed that Mesorhizobium is useful as a reducing agent of the negative effects of soil calcification on soil P nutrition. The ability to produce IAA by tested strains can have direct effects on production of phytohormones, enzymatic activities, nitrogen accumulation in shoots and seeds, and plant biomass [56][57][58][59]. The isolated strains showed high catalase activity, similar to the observations made by Nakayama et al [60], which indicated that these isolates could…”

Section: Discussionsupporting

confidence: 62%

Deciphering the Factors for Nodulation and Symbiosis of Mesorhizobium Associated with Cicer arietinum in Northwest India

et al. 2019

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The compatibility between rhizobia and legumes for nitrogen-fixing nodules and the stages of root hair curling, formation of infection thread, and nodulation initiation have been vitally studied, but the factors for the sustainable root surface colonization and efficient symbiosis within chickpea and rhizobia have been poorly investigated. Hence, we aimed to analyze phenotypic properties and phylogenetic relationships of root-nodule bacteria associated with chickpea (Cicer arietinum) in the north-west Indo Gangetic Plains (NW-IGP) region of Uttar Pradesh, India. In this study, 54 isolates were recovered from five agricultural locations. Strains exhibited high exopolysaccharide production and were capable of survival at 15–42 °C. Assays for phosphate solubilization, catalase, oxidase, Indole acetic acid (IAA) production, and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity revealed that all the tested isolates possessed plant growth-promoting potential. Metabolic profiling using Biolog plates indicated that patterns of substrate utilization differed considerably among isolates. A biofilm formation assay showed that isolates displayed a nearly four-fold range in their capacity for biofilm development. Inoculation experiments indicated that all isolates formed nodules on chickpea, but they exhibited more than a two-fold range in symbiotic efficiency. No nodules were observed on four other legumes (Phaseolus vulgaris, Pisum sativum, Lens culinaris, and Vigna mungo). Concatenated sequences from six loci (gap, edD, glnD, gnD, rpoB, and nodC) supported the assignment of all isolates to the species Mesorhizobium ciceri, with strain M. ciceri Ca181 as their closest relative.

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Section: Discussionsupporting

confidence: 62%

Deciphering the Factors for Nodulation and Symbiosis of Mesorhizobium Associated with Cicer arietinum in Northwest India

et al. 2019

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“…It has been recently shown a relevant interaction in field between Calcium and P solubilization process. Phosphate‐solubilizing bacteria reduce the negative effects of soil calcification on soil phosphorus (P) nutrition .…”

Section: Introductionmentioning

confidence: 88%

Exploring the response of Actinobacteria to the presence of phosphorus salts sources: Metabolic and co‐metabolic processes

et al. 2019

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liquid media containing Ca 3 PO 4 ; AlPO 4 or FePO 4 as the sole phosphate source, and discusses the possible mechanisms involved in this process. P solubilization by different strains was accompanied by a significant drop in pH from 7.0 to 2.15-5.0 after 14 days. The efficiency of different strains depended on the P-source. Streptomyces spp. MM140 and MM141 were the most efficient in solubilizing Ca 3 PO 4 , MM136, and MM141 were the most efficient in solubilizing AlPO 4 , while all strains were equally efficient in solubilizing FePO 4 . Gluconic, oxalic, citric, malic, succinic, formic, and acetic acid were detected in the medium with Ca 3 PO 4 , while all except acetic acid were detected in the media with FePO 4 or AlPO 4 . Although we did not use an organic source of phosphorus in the media, all strains produced acid and alkaline phosphatase. It is concluded from this study that actinobacteria produced multiple organic acids followed by a decrease in the pH to solubilize phosphate salts.As well as producing phosphatase, these microorganisms were found to have different ways of making P available, suggesting an ecological advantage as they form part of soil microbiomes important for plants. K E Y W O R D Sactinobacteria, phosphate solubilization, organic acids, Streptomyces

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“…Unambiguously, our results indicated that the inoculation with single or mix culture of PSP strains (ENPSB) were contributed to increase the amount of available phosphorus in the soil and this is well in agreement with results obtained by Liu et al (2014) who found that the highest level of available P and the greatest stimulation of plant height and dry weight were obtained in soils coinoculated with the three bacterial strains of phosphate dissolving bacteria. Shi et al (2017) and Adnan et al (2017) reported that the use of PSB strains increased P availability and reduced the negative effect of calcification on the soil.…”

Section: Discussionmentioning

confidence: 99%

Isolation and selection of highly effective phosphate solubilizing bacterial strains to promote wheat growth in Egyptian calcareous soils

et al. 2019

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Background: Forty phosphate solubilizing bacterial (PSB) isolates were isolated from the root zone of wheat plants cultivated in the Delta and the Northwestern coast regions of Egypt. All isolates proofed their ability to dissolve tricalcium phosphate on the National Botanical Research Institute's phosphate growth medium (NBRIP) by producing clear zone and increasing the available phosphorus that ranged between 40 and 707 mg l −1. They were designated as Egyptian native phosphate solubilizing bacteria (ENPSB). Results: All strains proofed their ability to dissolve tricalcium phosphate on (NBRIP) medium by producing clear zone and increasing the available phosphorus that ranged between 40 and 707 mg l −1. The ENPSB 1, 2, and 3 strains were highly efficient as they gave 707, 653, and 693 mg l −1 soluble phosphorus respectively. Intriguingly, the two highly efficient strains for phosphate solubilization were isolated from the Northwestern coast alkaline soils. Moreover, 75% of strains were also produced profitable amounts of indole acetic acid (IAA) ranged from 0.79 to 50.5 mg l −1. Amazingly, the most efficient strain ENPSB 1 in solubilizing phosphorus (707 mg l −1 soluble P) was the best one for producing IAA (50.5 mg l −1). The three efficient strains were identified using the sequencing of 16S rRNA. Sequence analysis of 16S rRNA for selected strains confirmed that the strains ENPSB 1, 2, and 3 were genetically closed to Enterobacter aerogenes; Pantoea sp. and Enteriobacter sp. respectively. Conclusion: The inoculation by mix cultures of strains (ENPSB 1, 2, and 3) contributed to raising the dry weight and P content of wheat plants by 76% and 12% over the full fertilized plants. Inoculation of soil PSB can be used to solve the deficiency of phosphorus and promote plant growth effectively in calcareous soils.

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Phosphate-Solubilizing Bacteria Nullify the Antagonistic Effect of Soil Calcification on Bioavailability of Phosphorus in Alkaline Soils

Cited by 105 publications

References 69 publications

Deciphering the Factors for Nodulation and Symbiosis of Mesorhizobium Associated with Cicer arietinum in Northwest India

Deciphering the Factors for Nodulation and Symbiosis of Mesorhizobium Associated with Cicer arietinum in Northwest India

Exploring the response of Actinobacteria to the presence of phosphorus salts sources: Metabolic and co‐metabolic processes

Isolation and selection of highly effective phosphate solubilizing bacterial strains to promote wheat growth in Egyptian calcareous soils

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