Phenylethanoid Glycoside Profiles and Antioxidant Activities of Osmanthus fragrans Lour. Flowers by UPLC/PDA/MS and Simulated Digestion Model

Abstract

Variations of phenylethanoid glycoside profiles and antioxidant activities in Osmanthus fragrans flowers through the digestive tract were evaluated by a simulated digestion model and UPLC/PDA/MS. Major phenylethanoid glycosides and phenolic acids, namely, salidroside, acteoside, isoacteoside, chlorogenic acid, and caffeic acid, were identified in four cultivars of O. fragrans flowers, and the concentration of acteoside was the highest, being up to 71.79 mg/g dry weight. After simulated digestion, total phenylethanoid glycoside contents and antioxidant activities were significantly decreased. Acteoside was identified as decomposing into caffeic acid, whereas salidroside was found to be stable during simulated digestion. According to Pearson's correlation analysis, acteoside contents showed good correlations with antioxidant activities during simulated digestion (R(2) = 0.994, P < 0.01). In conclusion, acteoside was the major contributor to the antioxidant activity of O. fragrans flowers, and salidroside was considered as the major antioxidant compound of O. fragrans flowers in vivo.

Origin Discrimination of Osmanthus fragrans var. thunbergii Flowers using GC–MS and UPLC‐PDA Combined with Multivariable Analysis Methods

“…The extracts were analysed by UPLC (ACQUITY, Waters, Milford, MA, USA) equipped with a photodiode array (PDA) detector (ACQUITY) and MS (Synapt G2, Waters, Milford, MA, USA), using a modification of method described by Jiang et al (2015). A BEH-C18 Symmetry column (100 mm × 2.1 mm; 1.7 μm) attached with a VanGuard precolumn (2.1 mm × 5 mm, 1.7 μm) (Waters, Milford, MA, USA) was maintained at 40°C.…”

“…The MS detector was operated in the electrospray ionisation (ESI) negative mode targeting phenolic compounds, which were the main biological compounds in O. fragrans flowers (Xiong et al, 2014;Jiang et al, 2015), under the following conditions: capillary voltage, 2.6 kV; sampling cone voltage, 40 V; extraction cone voltage, 4.0 V; source temperature, 100°C; desolvation temperature, 300°C; cone gas flow rate, 50 L/h; and desolvation gas, 600 L/h. An MS E method was used at a scan range from m/z 50 to m/z 1000.…”

“…Lour., a plant belonging to the Oleaceae family, is widely distributed in Asia, especially in the south and middle of China . Osmanthus fragrans flowers have been demonstrated to attenuate inflammation (Hung et al, 2013;Huang et al, 2015), possess antioxidant activity (Wu et al, 2009;Xiong et al, 2014;Jiang et al, 2015), protect against ageing (Xiong et al, 2016) and inhibit melanogenesis (Wu et al, 2009). With these benefits, the O. fragrans flowers are used as folk medicine and as additive for teas, beverages and foods, such as pastry, paste, vinegar and liqueurs , and the essential oil of the flowers is used for perfumes, flavours and aromatherapy.…”

Floral volatiles identification and molecular differentiation of Osmanthus fragrans by neutral desorption extractive atmospheric pressure chemical ionization mass spectrometry

“…Osmanthus fragrans Lour., O. fragrans, commonly referred to as sweet osmanthus, is a plant species widely cultivated in Eastern Asia that is well known for the very distinct and pleasant fragrance of its flowers. 1,2 O. fragrans is of high economic value in China. 2,3 The highly distinct floral fragrance of O. fragrans has been the subject of extensive research.…”

Total phenylethanoid glycosides and magnoloside IafromMagnolia officinalisvar.bilobafruits inhibit ultraviolet B-induced phototoxicity and inflammation through MAPK/NF-κB signaling pathways

“…10 Literature showed that phenylethanoid glycosides are found mainly in Orobanchaceae plants 12,13 and possess different pharmacological properties, including anti-inammatory, antioxidant, antitumor, antiviral, antibacterial, immunomodulatory, and neuroprotective effects. [14][15][16][17][18][19][20][21] Protective effects against UVB-induced phototoxicity are related to plant extracts which are rich in antioxidants. 22,23 The purpose of this study is to assess the inhibitory activity against UVB-induced phototoxicity and its underlying mechanisms of total phenylethanoid glycosides (TPG) and magnoloside I a (MI a ) from M. officinalis var.…”