Phenotypical Rescue of Bmp15 Deficiency by Mutation of Inhibin<i>α</i>(<i>inha</i>) Provides Novel Clues to How Bmp15 Controls Zebrafish Folliculogenesis

Zhai, Yue; Cheng, Zhao; Geng, Ruijing; Wu, Kun-Lung; Yuan, Ming; Ai, Nana; Ge, Wei

doi:10.1101/2022.12.30.522301

Cited by 2 publications

(4 citation statements)

References 108 publications

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“…Vldlr may be associated with formation of yolk oil droplets and Lrp13 with vitellogenin uptake ( Hiramatsu N et al, 2013 ). Other suggested vitellogenin receptor candidates, like lrp1ab, lrp2a, lrp5 and lrp6, were not expressed in our dataset ( Zhai et al, 2022 ), perhaps because oocytes larger than 40 µm were excluded.…”

Section: Resultsmentioning

confidence: 93%

“…Accumulation of bmp15 and gdf9 transcripts was detected only in 30dpf ZW germ cells, but not in 19dpf ZW germ cells or ZZ germ cells ( Figure 6O, P , Supplementary Figure S1A, S1B ). The loss of either Bmp15 or Gdf9 function blocks ovarian follicle development in zebrafish and mouse ( Dong et al, 1996 ; Su et al, 2004 ; Dranow et al, 2016 ; Chen et al, 2022 ; Zhai et al, 2022 ). The 30dpf ZW cells also accumulated transcripts encoding the Balbiani body protein Bucky ball ( buc ), an early marker of oocyte asymmetry ( Marlow and Mullins, 2008 ; Jamieson-Lucy et al, 2022 ), but few ZZ cells did, and only at a low level ( Figures 6Q, R ).…”

Section: Resultsmentioning

confidence: 99%

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Direct male development in chromosomally ZZ zebrafish

Wilson,

Batzel,

Postlethwait

2024

Front. Cell Dev. Biol.

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The genetics of sex determination varies across taxa, sometimes even within a species. Major domesticated strains of zebrafish (Danio rerio), including AB and TU, lack a strong genetic sex determining locus, but strains more recently derived from nature, like Nadia (NA), possess a ZZ male/ZW female chromosomal sex-determination system. AB fish pass through a juvenile ovary stage, forming oocytes that survive in fish that become females but die in fish that become males. To understand mechanisms of gonad development in NA zebrafish, we studied histology and single cell transcriptomics in developing ZZ and ZW fish. ZW fish developed oocytes by 22 days post-fertilization (dpf) but ZZ fish directly formed testes, avoiding a juvenile ovary phase. Gonads of some ZW and WW fish, however, developed oocytes that died as the gonad became a testis, mimicking AB fish, suggesting that the gynogenetically derived AB strain is chromosomally WW. Single-cell RNA-seq of 19dpf gonads showed similar cell types in ZZ and ZW fish, including germ cells, precursors of gonadal support cells, steroidogenic cells, interstitial/stromal cells, and immune cells, consistent with a bipotential juvenile gonad. In contrast, scRNA-seq of 30dpf gonads revealed that cells in ZZ gonads had transcriptomes characteristic of testicular Sertoli, Leydig, and germ cells while ZW gonads had granulosa cells, theca cells, and developing oocytes. Hematopoietic and vascular cells were similar in both sex genotypes. These results show that juvenile NA zebrafish initially develop a bipotential gonad; that a factor on the NA W chromosome, or fewer than two Z chromosomes, is essential to initiate oocyte development; and without the W factor, or with two Z doses, NA gonads develop directly into testes without passing through the juvenile ovary stage. Sex determination in AB and TU strains mimics NA ZW and WW zebrafish, suggesting loss of the Z chromosome during domestication. Genetic analysis of the NA strain will facilitate our understanding of the evolution of sex determination mechanisms.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Direct male development in chromosomally ZZ zebrafish

Wilson,

Batzel,

Postlethwait

2024

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…Vldlr may be associated with formation of yolk oil droplets and Lrp13 with vitellogenin uptake (Hiramatsu N et al, 2013). Other suggested vitellogenin receptor candidates, like lrp1ab, lrp2a, lrp5 and lrp6, were not expressed in our dataset (Zhai et al, 2022).…”

Section: Germ Cellsmentioning

confidence: 80%

“…Zhai et al, 2022, Chen et al, 2022). The 30dpf ZW cells also accumulated transcripts encoding the Balbiani body protein Bucky ball ( buc ), an early marker of oocyte asymmetry (Marlow and Mullins, 2008, Jamieson-Lucy et al, 2022), but few ZZ cells did, and only at a low level (Fig.…”

Section: Resultsmentioning

confidence: 99%

Direct Male Development in Chromosomally ZZ Zebrafish

Wilson,

Batzel,

Postlethwait

2023

Preprint

View full text Add to dashboard Cite

The genetics of sex determination varies across taxa, sometimes even within a species. Major domesticated strains of zebrafish (Danio rerio), including AB and TU, lack a strong genetic sex determining locus, but strains more recently derived from nature, like Nadia (NA), possess a ZZ male/ZW female chromosomal sex-determination system. AB strain fish pass through a juvenile ovary stage, forming oocytes that survive in fish that become females but die in fish that become males. To understand mechanisms of gonad development in NA zebrafish, we studied histology and single cell transcriptomics in developing ZZ and ZW fish. ZW fish developed oocytes by 22 days post-fertilization (dpf) but ZZ fish directly formed testes, avoiding a juvenile ovary phase. Gonads of some ZW and WW fish, however, developed oocytes that died as the gonad became a testis, mimicking AB fish, suggesting that the gynogenetically derived AB strain is chromosomally WW. Single-cell RNA-seq of 19dpf gonads showed similar cell types in ZZ and ZW fish, including germ cells, precursors of gonadal support cells, steroidogenic cells, interstitial/stromal cells, and immune cells, consistent with a bipotential juvenile gonad. In contrast, scRNA-seq of 30dpf gonads revealed that cells in ZZ gonads had transcriptomes characteristic of testicular Sertoli, Leydig, and germ cells while ZW gonads had granulosa cells, theca cells, and developing oocytes. Hematopoietic and vascular cells were similar in both sex genotypes. These results show that juvenile NA zebrafish initially develop a bipotential gonad; that a factor on the NA W chromosome or fewer than two Z chromosomes is essential to initiate oocyte development; and without the W factor or with two Z doses, NA gonads develop directly into testes without passing through the juvenile ovary stage. Sex determination in AB and TU strains mimics NA ZW and WW zebrafish, suggesting loss of the Z chromosome during domestication. Genetic analysis of the NA strain will facilitate our understanding of the evolution of sex determination mechanisms.

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Phenotypical Rescue of Bmp15 Deficiency by Mutation of Inhibinα(inha) Provides Novel Clues to How Bmp15 Controls Zebrafish Folliculogenesis

Cited by 2 publications

References 108 publications

Direct male development in chromosomally ZZ zebrafish

Direct male development in chromosomally ZZ zebrafish

Direct Male Development in Chromosomally ZZ Zebrafish

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