Phenotypic Differentiation of Bifidobacteria of Human and Animal Origins

Gavini, F.; Pourcher, Anne‐Marie; Neut, Christel; Monget, D.; Romond, C; Oger, C.; Izard, D.

doi:10.1099/00207713-41-4-548

Cited by 97 publications

(54 citation statements)

References 15 publications

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“…Because bifidobacteria were detected occasionally and in low counts in examined fish intestinal samples and species identified are considered as of human, animal or honeybee origin, we suppose that bifidobacteria were present only as environmental contamination from the water where fish stayed. Our conclusion is supported also by findings of Gavini et al (1991) who reported that Bifidobacterium species may be present in sewage or river water. They identified the isolates by API system and clustered by numerical analysis into groups with human, dog, pig, ruminant or rat bifidobacteria.…”

Section: Discussionsupporting

confidence: 81%

Occurrence of bifidobacteria and lactobacilli in digestive tract of some freshwater fishes

et al. 2012

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Distal parts of the fish intestine were analyzed for presence of bifidobacteria and lactobacilli using selective agars. Seventy seven samples from Cyprinus carpio, Oncorhynchus mykiss, Carassius auratus, Tinca tinca, Perca fluviatilis, Rutilus rutilus, Scardinius erythrophthalmus, Oreochromis niloticus, and Squalius cephalus were collected randomly throughout years 2008 and 2009. Bifidobacteria were detected in 5 samples from 4 fish species at counts 2.18-4.29 log CFU/g, lactobacilli were present in 6 fish species at counts 1.21-3.65 log CFU/g. Seven bifidobacterial isolates were identified to the species level using biochemical tests and by sequencing of 16S rRNA gene. Three strains belonged to species B. longum, two isolates were identified as B. dentium, one strain as B. asteroides and one isolate was not determined to the species level by employed methods. As identified bifidobacterial species are considered to be of human, animal or honeybee origin, they probably derived as contamination from sewage or other sources. After further more detail testing, the possible use of isolated bifidobacteria as probiotics is promising since they were able to pass through the digestive tract successfully.

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Section: Discussionsupporting

confidence: 81%

Occurrence of bifidobacteria and lactobacilli in digestive tract of some freshwater fishes

et al. 2012

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“…+, Positive; 2, negative; V, variable; ND, not determined. (Scardovi, 1986;Gavini et al, 1991;Crociani et al, 1996;Jian & Dong, 2002 …”

mentioning

confidence: 99%

Bifidobacterium psychraerophilum sp. nov. and Aeriscardovia aeriphila gen. nov., sp. nov., isolated from a porcine caecum

Simpson

Ross

Fitzgerald

et al. 2004

International Journal of Systematic and Evolutionary Microbiology

108

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In a previous study that was based primarily on 16S rDNA sequencing, two groups of bifidobacteria that had been recovered from a pig caecum were proposed to belong to two novel species, termed ‘Bifidobacterium pyschroaerophilum’ and ‘Bifidobacterium aerophilum’. In this study, based on DNA G+C content and partial heat-shock protein 60 (HSP60) gene sequences, the assignment of ‘B. pyschroaerophilum’, corrected to Bifidobacterium pyschraerophilum, to the genus Bifidobacterium was confirmed. The DNA G+C content of ‘B. aerophilum’ was relatively low, which was consistent with its segregation into subcluster II of the 16S rDNA phylogenetic tree. Based on partial 16S rDNA and HSP60 gene sequences, the species was transferred to a novel genus and reclassified as Aeriscardovia aeriphila gen. nov., sp. nov. Biochemical profiles and growth parameters were established for both novel species. Interestingly, each had a high tolerance to oxygen and grew on agar media under aerobic conditions, a trait that may relate to their caecal habitat. Under aerobic growth conditions, the short-rod morphology of A. aeriphila lengthened considerably. This appeared to arise from incomplete cell division. In addition, B. pyschraerophilum was unusual in that it grew at temperatures as low as 4 °C. On the basis of genetic, phylogenetic and phenotypic data, the identities of Bifidobacterium pyschraerophilum sp. nov. (type strain, T16T=LMG 21775T=NCIMB 13940T) and Aeriscardovia aeriphila gen. nov., sp. nov. (type strain, T6T=LMG 21773T=NCIMB 13939T) are confirmed.

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“…(i) Cluster I. It was interesting that one of the strains (CUETM 89-271) isolated from child feces was also studied by Gavini et al (10) and clustered in their group VIIa but was not assigned to any known species. In this study, strain DSM 20097 (= C10-45) was unclustered in cluster I.…”

Section: (20)mentioning

confidence: 99%

Phenotypic and Genomic Analyses of Human Strains Belonging or Related to Bifidobacterium longum, Bifidobacterium infantis, and Bifidobacterium breve

Bahaka

Neut²,

Khattabi

et al. 1993

International Journal of Systematic Bacteriology

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A numerical analysis based on phenotypic characteristics (89 enzymatic tests and 49 carbohydrate acidification tests), in which experimental strips from Biomerieux-API, La Balme les Grottes, France, were used, was performed to characterize 82 new isolates belonging or related to Bijidobacterium longum, Bifidobacteriurn infantis, and Bafuiobucteriurn breve. A total of 72 strains were isolated from child or adult feces, and the other strains were obtained from human vaginas and bronchi. In this study we also included 38 type and reference strains that were representative of all species of the genus Bifidobucterium and 6 strains belonging to the genus LactobuciUus, DNA-DNA relationships between B. longurn and B. infantis were determined by using 19 strains related to these species, as determined by the numerical analysis, The degree of DNA binding was determined by the S1 nuclease method. The phenotypic study revealed that there were six main clusters, which were subdivided into nine subclusters. Subcluster Va contained the type strains of B. longum and B. infantis. The DNA-DNA relatedness values of some of the new isolates were very similar to the DNA-DNA relatedness values of the type strain of B. longurn. On the basis of these data, it was difficult to isolate B. infantis strains and then to define B. infantis as a single species separated from B. longurn. Subclusters lVb to IVf comprised reference strains of B. breve. Cluster I11 and subcluster Ia were not identified.At the species, present time, the genus Bifidobacterium includes 29 10 of which are of human origin. Four of these species, Bijidobacterium bijidum, Bijidobacterium longum, Bifidobacterium infantis, and Bifidobacterium breve, have been studied with increasing interest because of their role in physiological relationships in human (especially infant and child) gastrointestinal tracts (2,3,29). Furthermore, today, the use of these bacteria in food microbiology (dairy products) makes accurate differentiation of these organisms necessary (19).The type species of the genus, B. bifidum, is well separated from the other species on the basis of its phenotypic characteristics, as well as its DNA-DNA hybridization values. Identification of the three other species studied in this work is much more uncertain; in particular, distinction between B. longum and B. infantis is difficult.On the basis of the results of phenotypic studies, Reuter (18) defined and subdivided B. longum into two biovars, biovars a and b, and Mitsuoka (17)

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Phenotypic Differentiation of Bifidobacteria of Human and Animal Origins

Cited by 97 publications

References 15 publications

Occurrence of bifidobacteria and lactobacilli in digestive tract of some freshwater fishes

Occurrence of bifidobacteria and lactobacilli in digestive tract of some freshwater fishes

Bifidobacterium psychraerophilum sp. nov. and Aeriscardovia aeriphila gen. nov., sp. nov., isolated from a porcine caecum

Phenotypic and Genomic Analyses of Human Strains Belonging or Related to Bifidobacterium longum, Bifidobacterium infantis, and Bifidobacterium breve

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