Phenotypic characteristics of adipocytes generated from Meckel's chondrocytes in response to chick serum in vitro

Ishizeki, Kiyoto; Takahashi, Noriaki; Nawa, Tokio

doi:10.1007/s00441-002-0541-z

Cited by 6 publications

(1 citation statement)

References 32 publications

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“…Although MSCs are fully committed to a developmental lineage, several studies have shown that MSC-derived cells can switch to another cell lineage or return to an uncommitted developmental stage [6], [7], [8], [9], [10]. Likewise, the signalling molecules and pathways leading to transdifferentiation (i.e.…”

Section: Introductionmentioning

confidence: 99%

Increased Adipogenesis in Cultured Embryonic Chondrocytes and in Adult Bone Marrow of Dominant Negative Erg Transgenic Mice

et al. 2012

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In monolayer culture, primary articular chondrocytes have an intrinsic tendency to lose their phenotype during expansion. The molecular events underlying this chondrocyte dedifferentiation are still largely unknown. Several transcription factors are important for chondrocyte differentiation. The Ets transcription factor family may be involved in skeletal development. One family member, the Erg gene, is mainly expressed during cartilage formation. To further investigate the potential role of Erg in the maintenance of the chondrocyte phenotype, we isolated and cultured chondrocytes from the rib cartilage of embryos of transgenic mice that express a dominant negative form of Erg (DN-Erg) during cartilage formation. DN-Erg expression in chondrocytes cultured for up to 20 days did not affect the early dedifferentiation usually observed in cultured chondrocytes. However, lipid droplets accumulated in DN-Erg chondrocytes, suggesting adipocyte emergence. Transcriptomic analysis using a DNA microarray, validated by quantitative RT-PCR, revealed strong differential gene expression, with a decrease in chondrogenesis-related markers and an increase in adipogenesis-related gene expression in cultured DN-Erg chondrocytes. These results indicate that Erg is involved in either maintaining the chondrogenic phenotype in vitro or in cell fate orientation. Along with the in vitro studies, we compared adipocyte presence in wild-type and transgenic mice skeletons. Histological investigations revealed an increase in the number of adipocytes in the bone marrow of adult DN-Erg mice even though no adipocytes were detected in embryonic cartilage or bone. These findings suggest that the Ets transcription factor family may contribute to the homeostatic balance in skeleton cell plasticity.

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Section: Introductionmentioning

confidence: 99%

Increased Adipogenesis in Cultured Embryonic Chondrocytes and in Adult Bone Marrow of Dominant Negative Erg Transgenic Mice

et al. 2012

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Adipogenic differentiation of chicken epithelial oviduct cells using only chicken serum

Khuong

Jeong

2011

In Vitro Cell.Dev.Biol.-Animal

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Chicken epithelial oviduct cells (COCs) are part of important supportive tissues in chicken reproductive organs responsible for secretion of the majority of chicken egg protein. In chickens, the biological process of adipocyte differentiation has been extensively studied in vitro using a number of cell types including a preadipocyte precursor cell line, a number of other undifferentiated cell lines, and chicken embryonic fibroblasts. On the contrary, adipogenic differentiation in epithelial cells has not yet been achieved. In our study, we induced COCs to differentiate into adipocytes using chicken serum at concentrations of 5% and 10%. After a 24-h culture period at 37°C in a humidified 5% CO(2) atmosphere, oviduct cell morphology changed dramatically through formation of lipid droplets, observed by Oil Red O staining. Also, chicken serum strongly induced 3T3-L1 preadipocyte cell differentiation into adipocyte. In addition, mRNA expression levels of peroxisome proliferator-activated receptor gamma, adipocyte fatty acid-binding protein (aP2), and CCAAT-enhancer-binding protein alpha were significantly increased 48 h after induction. These results suggest that COCs can be induced to differentiate into adipocyte-like cells. Moreover, through this study, we confirmed that chicken serum is an effective adipocyte differentiation-inducing agent. Our findings may provide a unique model for studying and applying chicken transdifferentiation and adipocyte differentiation.

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Fatty Acids Have Different Adipogenic Differentiation Potentials in Stromal Vascular Cells Isolated from Abdominal Fat in Laying Hens

Regassa

Suh

Datar

et al. 2017

Lipids

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This study was conducted to examine the effects of fatty acids (FA) with/without chicken serum (CS) on the expression of adipogenic transcripts and adipogenesis in chicken stromal vascular cells (SVC). In experiment 1, SVC were grown in DMEM containing 10% FBS (Control) and treated with 300 µM oleic acid (OLA) + FBS, linoleic acid (LNA) + FBS, palmitic acid (PAM) + FBS, or stearic acid (STA) + FBS for 48 h. In experiment 2, cells were grown in DMEM containing 5% CS and treated with 300 µM OLA (CS + OLA), PAM (CS + PAM), STA (CS + STA) or 200 µM LNA (CS + LNA) for 48 h. Adipogenesis was determined using Oil Red O staining and glycerol-3-phosphate dehydrogenase (GPDH) activity. The proportion of OLA, PAM, or STA was increased (P < 0.05) in SVC grown in either FBS or CS with OLA, PAM or STA. Adipogenesis was induced in FBS + OLA, FBS + LNA, FBS + PAM, FBS + STA, CS + OLA, CS + LNA, CS + PAM, or CS + SAT compared to FBS. GPDH activity was significantly higher in FBS + OLA and FBS + LNA than one in FBS. Compared to FBS, the expression of FABP4 mRNA increased (P < 0.05) in FBS + OLA, FBS + LNA, or FBS + PAM, whereas that of C/EBPα, C/EBPβ, and ATGL increased (P < 0.05) in FBS + OLA or FBS + LNA cells. Expression of FABP4 and C/EBPβ mRNA was higher in CS, CS + OLA, CS + LNA, CS + PAM, or CS + SAT compared with (FBS, whereas the expression of ATGL and C/EBPα was higher in CS, CS + OLA, or CS + LNA than FBS cells. In conclusion, these results showed that FA have different potentials to induce adipogenesis, LNA is the most potent among the tested FA, and these potentials can be improved in the presence of CS.

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Phenotypic characteristics of adipocytes generated from Meckel's chondrocytes in response to chick serum in vitro

Cited by 6 publications

References 32 publications

Increased Adipogenesis in Cultured Embryonic Chondrocytes and in Adult Bone Marrow of Dominant Negative Erg Transgenic Mice

Increased Adipogenesis in Cultured Embryonic Chondrocytes and in Adult Bone Marrow of Dominant Negative Erg Transgenic Mice

Adipogenic differentiation of chicken epithelial oviduct cells using only chicken serum

Fatty Acids Have Different Adipogenic Differentiation Potentials in Stromal Vascular Cells Isolated from Abdominal Fat in Laying Hens

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