Phenotypic and Molecular Detection of β-lactamase Genes blaTEM, blaCTX, and blaSHV Produced by Salmonella spp. Isolated from Poultry Meat

Ghazaei, Ciamak

doi:10.5812/gct.84367

Cited by 8 publications

(11 citation statements)

References 24 publications

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“…The antibiotic resistance pattern of typhoid and nontyphoidal Salmonella enterica serovars observed in this study has therapeutic and public health implications. Before the 1980s, when multidrug resistance was reported in Salmonella pathogens, Salmonella infections were treated with ampicillin, chloramphenicol and trimethoprim/sulfamethoxazole (Ghazaei, 2018). With the high incidence of therapy failure with the first‐generation antimicrobials, ciprofloxacin is now primarily used in the treatment of clinical salmonellosis in humans, while third generations cephalosporin and azithromycin have also been used in some cases (Le Hello et al 2011; Le Hello et al 2013; Raufu et al 2013 Akinyemi et al 2018; Ghazaei, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Before the 1980s, when multidrug resistance was reported in Salmonella pathogens, Salmonella infections were treated with ampicillin, chloramphenicol and trimethoprim/sulfamethoxazole (Ghazaei, 2018). With the high incidence of therapy failure with the first‐generation antimicrobials, ciprofloxacin is now primarily used in the treatment of clinical salmonellosis in humans, while third generations cephalosporin and azithromycin have also been used in some cases (Le Hello et al 2011; Le Hello et al 2013; Raufu et al 2013 Akinyemi et al 2018; Ghazaei, 2018). These have led to the emergence of ciprofloxacin and third‐generation cephalosporin resistance to Salmonella clinically and in the environment.…”

Section: Discussionmentioning

confidence: 99%

“…Antimicrobial resistance in Salmonella serovars is directly related to the production of ESBLs (Ghazaei, 2018), majorly TEM ( Temoneira ), SHV (containing sulfhydryl variable active sites) and CTX‐M (cefotaximase hydrolyzing activity). This study detected bla TEM in all the Salmonella serovars except Salmonella Pullorum.…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial resistance and virulence genes inSalmonella entericaserovars isolated from droppings of layer chicken in two farms in Nigeria

Shittu

Uzairue

Ojo

et al. 2022

Journal of Applied Microbiology

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Aim This study aimed to investigate the isolation rate, antibiotic resistance and virulence genes of Salmonella enterica serovar from two commercial farms in Nigeria. Methods and Results Salmonella isolation was performed according to the United States Food and Drug Agency (USFDA) method. Serotyping, antimicrobial susceptibility testing, detection of resistance and virulence genes were done using the Kauffman–White Scheme, disc diffusion, minimum inhibitory concentration and real‐time polymerase chain reaction techniques. Salmonella serovars were isolated from only farm A at 22/50 (44.0%) while none were isolated from farm B. Salmonella Typhi, 9 (40.9%); Salmonella Typhimurium, 2 (9.1%), Salmonella Enteritidis, 2 (9.1%), Salmonella Pullorum, 1 (4.5%), Salmonella Kentucky, 4 (18.2%) were identified while 4 (18.2%) were untypable. Sixteen isolates (72.7%) showed multiple drug resistance and 17 different resistance profile types with AMP‐CHL‐TRM‐SXT as the most prevalent pattern. Resistance genes (blaTEM, 12/22 (54.5%) and virulence genes (InvA, sopB, mgtC and spi4D, 22/22 (100.0%), ssaQ, 16/22 (72.7%) and spvC, 13/22 (59.1%) were found, while blaSHV, blaCTX‐M, floR, tetA, tetB, tetG and LJSGI‐1 genes were absent. Conclusion Pathogenic Salmonella were isolated from the chicken droppings in this study. Most of these strains were resistant to antibiotics and possessed characteristics of virulence. Significance and Impact of the Study Chicken droppings from this study area contained pathogenic strains of Salmonella and a rare occurrence of Salmonella Typhi. The study revealed that the environment and the food chain could be at risk of contamination of highly virulent and antimicrobial‐resistant strains of Salmonella. These could affect the profitability of the poultry industry and food consumption. There is a need for caution in indiscriminate disposal of poultry waste and the use of uncomposted chicken droppings in soil amendment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Antimicrobial resistance and virulence genes inSalmonella entericaserovars isolated from droppings of layer chicken in two farms in Nigeria

Shittu

Uzairue

Ojo

et al. 2022

Journal of Applied Microbiology

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“…Pure bacterial cultures from the SSA plate and Listeria selective agar base supplemented with Listeria Selective Supplement II (FD063 or FD063I) after resuscitation was obtained by streaking on freshly prepared nutrient plate agar and incubated for 24 hours. The obtained pure culture of the respective organisms were further characterized and identified based on their morphological and biochemical characteristics [18][19][20][21] as well as genomics features.…”

Section: Identification Of the Listeria And Salmonella Speciesmentioning

confidence: 99%

Antibiogram of Listeria and Salmonella Species Isolated from Tilapia Fish (Oreochromis nitolicus) and Snail (Archachatina marginata) Sold in Port Harcourt, Nigeria

Odu

Ogbonna

Daminabo

2021

JAMB

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Land snails are ubiquitous creatures found on farms, forests and domesticated in homes. Bacteriological studies indicate that various potentially pathogenic bacteria inhabit different organs and tissues including the haemolymph of the African giant snail, the presence of pathogenic bacteria in the snails are a health threat to consumers and may cause food borne diseases/ infection. Pollution of aquatic environments with organic waste of animal and human origin may lead to transfer of pathogens to the fishes, thereby making them carriers of the pathogens. In this study antibiogram studies were carried out on the isolates from snail and tilapia fish to determine antibiogram of Listeria monocytogenes and Salmonella species isolated from tilapia fishes and snails sold in Port Harcourt. One hundred and thirty two (132) samples were obtained from three different markets namely; Creek road, Mile one and Rumuokoro markets over a period of six months. Standard analytical protocols were employed to determine the bacteriological characteristics of the various parts such as intestine, flesh/meat, gills and fluid(snail). Antibiogram of Listeria and Salmonella species were determined using standard methods as recommended by CLSI. Statistical analyses were carried out using ANOVA and All pairs tukey-kramer. Results of Total heterotrophic bacterial count shows that snail samples had the highest number of bacterial count compared to frozen tilapia fishes. Mean Listeria spp. count for frozen tilapia fish ranged from 2.7 ±0.68 x104cfu/g to 2.9 ±0.23 x104cfu/g (flesh), 3.3 ±0.15 x104 to 3.7 ±0.35 x104cfu/g (gill), 3.8 ±0.44 x104 to 4.3 ±0.57 x104 cfu/g (Intestine), across the three markets, Mean Listeria spp. count for snail sample ranged from 0.7 ±0.29 x104 to 1.1 ±0.18 x104cfu/ml (Snail fluid) 1.1 ±0.18 x104 to 1.2 ±0.16 x104cfu/g (meat), 1.6 ±0.44 x104 to 1.9 ±0.57 x104cfu/g (Intestine) Creek road market. Mean total Salmonella count for frozen tilapia fish ranged from 1.0 ±0 x103cfu/g to 1.3 ±0.58 x103 (flesh), 1.0 ± 0 x103 to 1.6 ± 0.58 x103cfu/g (gills), 1.2 ±0.5 x103 cfu/g to 2 .0 ±1.41 x103 cfu/g (Intestine), across the three markets. Mean total Salmonella count for snail ranged from 1.0 ±0.58 x103 to 1.3 ±0 x103cfu/ml (fluid), 1.5 ±1.0 x103 to 1.7 ±0.96 x103cfu/g (meat), 1.7 ±0.96 x103cfu/g to 3.0 ±1.58 x103cfu/g (Intestine), across the three markets. Results of antibiogram revealed that all the Listeria species were 100% susceptible to Levofloxacin, Ofloxacin, Gentamycin, Azithromycin, Erythromycin and Ceftriaxone-sulbactam but 100% resistance to Augmentin, Ciprofloxacin and Cefuxime while Salmonella species were 100% susceptible to Ofloxacin and Ciprofloxacin and resistance to Cetazidime and Gentamicin, thus, these drugs should be considered the drug of choice for infections caused by these bacteria,

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“…Irrespective of the stringent measures implemented in the developed countries to control salmonellosis on poultry farms and retailing outlets, Salmonella has been reported at a prevalence that ranged from 4 to 20% [10,11]. TEM (Temoneira), SHV (sulfhydryl variable active sites) and CTX-M (cefotaximase hydrolyzing activity) beta-lactamases have been variously reported in Salmonella species [12][13][14][15]. Currently, many countries have witnessed a rise in the CTX-M variants [16,17].…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and molecular characterization of multidrug-resistant extended-spectrum beta-lactamase-producing Salmonella prevalent in raw chicken meat vended in Nigerian markets

Ogu

Akinnibosun

Imade

2021

Ovidius University Annals of Chemistry

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In Nigeria, there is still a scarcity of data on the recovery of multidrug-resistant ESBL-producing Salmonella in chicken meat. Hence this study characterized the probable multidrug-resistant extended-spectrum beta-lactamase-producing Salmonella prevalent in chilled raw chicken meat vended in Nigerian markets. Detection of Salmonella was performed by meat rinse centrifugation-plating technique. Presumptive Salmonella colonies were identified by phenotypic and 16S rRNA gene sequencing. The confirmed Salmonella isolates were tested for multidrug resistance by the Kirby Bauer disc diffusion test. Detection and confirmation of extended-spectrum beta-lactamase (ESBL) phenotypes were performed by double disc synergy and combination disc tests. PCR and DNA sequencing of the ESBL-encoding genes (bla SHV, bla TEM, and bla CTX-M) were also performed. The conserved and three-dimensional (3D) domains in ESBLs were respectively characterized by the reverse position-specific BLAST (RPS-BLAST) and Cn3D modeling tool. Of the 229 presumptive Salmonella isolates examined, 52 isolates were confirmed as Salmonella species, 46 isolates were multidrug-resistant and 41 isolates confirmed as multidrug-resistant ESBL-producing Salmonella species. The main serotypes were Salmonella enterica subsp. enterica serovar Typhimurium (35/52; 67.31%) and Salmonella enterica subsp. enterica serovar Enteritidis (17/52; 32.69%). Overall, the prevalence of chilled raw chicken meat contaminated with Salmonella was estimated at 0.17 (40/240). This value of prevalence exceeded the limits (≤ 0.1) set by the Meat Industry Guide, United Kingdom. All CTX-M, TEM, and SHV beta-lactamases produced by the Salmonella isolates were confirmed by RPS-BLAST and Cn3D modeling tool as serine-based hydrolases that consisted of two 3D domains with unique ligands such as sodium ion, formic acid, and glycerol. This study showed that multidrug-resistant ESBL-producing Salmonella was widespread in raw chicken meat vended in Nigerian markets. Thus, there is a need for relevant regulatory agencies to enforce safety.

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Phenotypic and Molecular Detection of β-lactamase Genes blaTEM, blaCTX, and blaSHV Produced by Salmonella spp. Isolated from Poultry Meat

Cited by 8 publications

References 24 publications

Antimicrobial resistance and virulence genes inSalmonella entericaserovars isolated from droppings of layer chicken in two farms in Nigeria

Antimicrobial resistance and virulence genes inSalmonella entericaserovars isolated from droppings of layer chicken in two farms in Nigeria

Antibiogram of Listeria and Salmonella Species Isolated from Tilapia Fish (Oreochromis nitolicus) and Snail (Archachatina marginata) Sold in Port Harcourt, Nigeria

Phenotypic and molecular characterization of multidrug-resistant extended-spectrum beta-lactamase-producing Salmonella prevalent in raw chicken meat vended in Nigerian markets

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