Phenotypic and Molecular Characterization ofMadurella pseudomycetomatissp. nov., a Novel Opportunistic Fungus Possibly Causing Black-Grain Mycetoma

Abstract

A case of black-grain mycetoma occurring on the lower jaw with an odontogenic origin, which to our knowledge is the first case reported in China, is presented here. The clinical manifestation, histopathological morphology, and microbiological features are described. The new species, Madurella pseudomycetomatis, isolated from the black grains discharged by this patient, was analyzed using sequence data of the multiloci of ribosomal DNA (rDNA) and its ability to ferment carbohydrate as well as morphology. The analyses of the internal transcribed spacer (ITS) region and the D1/D2 hypervariable region of the 28S ribosomal gene sequences support a new species designation. Antifungal susceptibility testing was conducted, indicating that Madurella pseudomycetomatis was highly susceptible to itraconazole, voriconazole, and amphotericin B; moderately susceptible to terbinafine; and resistant to fluconazole and flucytosine.

New Species of Madurella, Causative Agents of Black-Grain Mycetoma

“…Only Madurella grisea and M. mycetomatis were considered to be valid species. Recently, Yan et al (2010) added a novel species, Madurella pseudomycetomatis (19), but this name was considered a nomen invalidum because the nomenclatural type was not indicated and no Latin description was given (16). We corrected this here, and the name M. pseudomycetomatis was reintroduced.…”

“…A total of 21 species have been described in the genera Madurella and Indiella. de Hoog et al (8) (19). Madurella grisea Mackinnon et al is unrelated, being a member of Pleosporales (7).…”

Eumicetoma: actualidades y perspectivas

“…Un análisis filogenético reciente demostró que M. mycetomatis y M. grisea pertenecen a diferentes órdenes; en la actualidad, el género Madurella se encuentra constituido por M. mycetomatis, M. pseudomycetomatis, M. tropica y M. fahalii, que pertenecen al orden Sordiales, mientras que M. grisea es miembro de los Pleosporales y pasó a denominarse Trematosphaeria grisea 29,30,52,53 . Además, cuatro cepas dematiáceas de micelio estéril CBS obtenidas de pacientes con micetoma, originalmente identificadas por métodos tradicionales como M. mycetomatis, mostraron diferencias significativas en la región ITS, demostrándose que representan especies diferentes; ninguna de estas cepas era originaria de África 28 .…”

Madurella pseudomycetomatis: an Invalidly Published Name

“…The taxon Madurella pseudomycetomatis was recently proposed as a new species in a paper by Yan et al (2). It was isolated from black granules of the discharging sinuses on the lower jaw of a 27-year-old patient from China.…”

“…It was isolated from black granules of the discharging sinuses on the lower jaw of a 27-year-old patient from China. Based on sequence data of the multiloci of ribosomal DNA (rDNA), the ability of the isolate to ferment some carbohydrates, as well as analyses of the internal transcribed spacer (ITS) region and D1/D2 hypervariable region of the 28S ribosomal gene sequences, the isolate was considered by the authors to be sufficiently different to warrant its designation as a new species (2).…”