Phenotypic and genotypic diversity of Pectobacterium carotovorum subsp carotovorum causing soft rot disease of potatoes in Morocco

Faquihi, H.; Terta, Meriam; Amdan, Mohamed; Achbani, El Hassan; Ennaji, Moulay Mustapha; Mhand, Rajaa Aït

doi:10.1007/s10658-015-0732-6

Cited by 11 publications

(9 citation statements)

References 30 publications

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“…P. atrosepticum , P. carotovorum and P. brasiliense were described in Morocco as the main causative agents of blackleg and soft rot diseases in potato crop [ 40 , 41 , 42 , 43 ]. In 2016, D. dianthicola was described for the first time in the North of Morocco [ 44 ].…”

Section: Introductionmentioning

confidence: 99%

Diversity of Pectobacteriaceae Species in Potato Growing Regions in Northern Morocco

Oulghazi

Moumni

Khayi³

et al. 2020

Microorganisms

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Dickeya and Pectobacterium pathogens are causative agents of several diseases that affect many crops worldwide. This work investigated the species diversity of these pathogens in Morocco, where Dickeya pathogens have only been isolated from potato fields recently. To this end, samplings were conducted in three major potato growing areas over a three-year period (2015–2017). Pathogens were characterized by sequence determination of both the gapA gene marker and genomes using Illumina and Oxford Nanopore technologies. We isolated 119 pathogens belonging to P. versatile (19%), P. carotovorum (3%), P. polaris (5%), P. brasiliense (56%) and D. dianthicola (17%). Their taxonomic assignation was confirmed by draft genome analyses of 10 representative strains of the collected species. D. dianthicola were isolated from a unique area where a wide species diversity of pectinolytic pathogens was observed. In tuber rotting assays, D. dianthicola isolates were more aggressive than Pectobacterium isolates. The complete genome sequence of D. dianthicola LAR.16.03.LID was obtained and compared with other D. dianthicola genomes from public databases. Overall, this study highlighted the ecological context from which some Dickeya and Pectobacterium species emerged in Morocco, and reported the first complete genome of a D. dianthicola strain isolated in Morocco that will be suitable for further epidemiological studies.

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Section: Introductionmentioning

confidence: 99%

Diversity of Pectobacteriaceae Species in Potato Growing Regions in Northern Morocco

Oulghazi

Moumni

Khayi³

et al. 2020

Microorganisms

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“…In this region the majority of tuber seeds were imported and the irrigation water derived from a dam: either one or both agronomic practices would contribute to a diversity of pathogens wider in the Larache region (S4) than in the other investigated regions. While previous studies identified P. carotovorum species as the most prevalent soft rot pathogens in Morocco [36,37], our study extended the diversity of the other Pectobacterium and Dickyea, including the recently described species P. versatile [7]. This species encompasses isolates, including P. versatile strain SCC1 isolated in 1980 in Poland [57], which had been collected from a wide diversity of environments (host plants, surface waters) and geographic areas [7].…”

Section: Moroccomentioning

confidence: 54%

“…Analysis of the functions encoded by these DNA regions allows the prediction of species-specific metabolic traits: this knowledge contributes to the understanding of both the taxonomy and ecology of the Dickeya and Pectobacterium pathogens [21, [25][26][27]34]. P. atrosepticum, P. carotovorum and P. brasiliense were described in Morocco as the main causative agents of blackleg and soft rot disease in potato crop [35][36][37][38]. In 2016, D. dianthicola was described for the first time in the North of Morocco [39].…”

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confidence: 99%

Dickeya dianthicola emerged from a potato culture area exhibiting a wide diversity of pectinolytic pathogens in northern Morocco

Oulghazi¹,

Moumni²,

Khayi³

et al. 2020

Preprint

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Background: Dickeya and Pectobacterium are pectinolytic pathogens that cause damage to many plants including major crops. Emergence of D. solani and re-emergence D. dianthicola were recently observed in potato fields in several continents. The purpose of this work was to describe the species diversity of Dickeya and Pectobacterium collected from potato fields in Northern Morocco, where Dickeya potato pathogens have not been isolated until recently. Results: Along three years, 119 pathogens belonging to Pectobacterium and Dickeya genera were isolated from three potato culture areas and characterized using selective PCR and gapA gene sequencing. Out of them, 19% belonged to P. versatile, 3% to P. carotovorum, 5% to P. polaris, 56% to P. brasiliense, while 17% to D. dianthicola. The taxonomic assignations were confirmed by draft genome analyses of representative isolates belonging to the collected species. D. dianthicola were isolated from a unique area where a wide species diversity of pectinolytic pathogens was also observed. In potato maceration assay, D. dianthicola isolates were more aggressive than Pectobacterium isolates, a feature that should alert stakeholders of a potential threat linked to the emergence of D. dianthicola in this country. By combining Oxford Nanopore Technologies (ONT) and Illumina technologies, the sequence of the complete genome of D. dianthicola LAR.16.03.LID was obtained. A unique circular chromosome of 4,976,211 bp codes for 4,223 predicted proteins. Comparison of the three complete genomes of D. dianthicola strains RNS049, ME23 and LAR.16.03.LID revealed a highly conserved synteny and the occurrence of strain-specific regions associated with the presence of mobile elements. Conclusion: By combining population sampling and genomics, this study highlighted the ecological context from which D. dianthicola emerged in Morocco. Furthermore, the first complete genome of a D. dianthicola strain isolated in Northern Morocco will constitute a reference genome for investigating the dynamics of the emerging D. dianthicola pathogens in this country.

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“…The strain 2066-7 also revealed its contribution to improving the growth and yield of some crops such as Potato culture (Faquihi et al 2014). This strain 2066-7 was revealed an antagonist effect against other phytopathogenic bacteria (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Pantoea agglomerans Strains Isolated from Olive Knot disease in Morocco

Bouaichi.¹

2017

Biolife

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On the base of prospecting conducted during the agriculture season 2012-2013, the results revealed that the olive knot disease caused by Pseudomonas savastanoi pv.savastanoi was observed in different olive groves in Morocco. The disease incidence varies with location and cultivar. However, high disease incidence was reported in Taounate, Ouazzane and Meknes. The biochemical and the molecular tests were revealed the presence of saprophytic bacteria; Pantoea agglomerans, Enterobacter sp., Pseudomonas sp., associated with Pseudomonas savastanoi pv.savastanoi. Moreover, a hypersensitive reaction on tobacco leaves caused by Pantoea agglomerans strains (2074-1 and 2074-4) was noted. Nonetheless, the strain of Pantoea agglomerans (2066-7) did not affect the tobacco leaves.

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Phenotypic and genotypic diversity of Pectobacterium carotovorum subsp carotovorum causing soft rot disease of potatoes in Morocco

Cited by 11 publications

References 30 publications

Diversity of Pectobacteriaceae Species in Potato Growing Regions in Northern Morocco

Diversity of Pectobacteriaceae Species in Potato Growing Regions in Northern Morocco

Dickeya dianthicola emerged from a potato culture area exhibiting a wide diversity of pectinolytic pathogens in northern Morocco

Characterization of Pantoea agglomerans Strains Isolated from Olive Knot disease in Morocco

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