Phenomenological principles of microbial lignin degradation

Härtig, Claus; Lorbeer, Helmut

doi:10.1002/abio.370130107

Cited by 2 publications

(3 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5). As expected, with the transition to idiophase, the biomass started to decline (11,14). Unexpectedly, even though biomass reductions were similar in the case of the OEssp1 and PC9 strains, it was significantly slower (by 20 to 27%) in the KDssp1 strain.…”

Section: Figsupporting

confidence: 70%

“…This observation was further validated by monitoring the expression of selected genes over time. There was a shift in the production of ligninolytic enzymes and peptidases, which are considered markers for idiophase in WRF (12)(13)(14)(15)27). Many CAZymes were also strongly influenced by the transition to idiophase and Ssp1 manipulation.…”

Section: Figmentioning

confidence: 99%

“…Many CAZymes were also strongly influenced by the transition to idiophase and Ssp1 manipulation. Although the biphasic growth of WRF is known and has been investigated for many years, most of the knowledge accumulated is based on growth conditions and nutrient limitations as triggers of the shift from trophophase to idiophase (10)(11)(12)(13)(14). Since the OEssp1 strain showed an expression pattern resembling the idiophase previous to glucose limitation, this suggests that Ssp1 may act as an additional regulator for the transition of P. ostreatus to the secondary growth phase.…”

Section: Figmentioning

confidence: 99%

See 2 more Smart Citations

Manipulating the Expression of Small Secreted Protein 1 (Ssp1) Alters Patterns of Development and Metabolism in the White-Rot Fungus Pleurotus ostreatus

Feldman

Amedi

Carmeli

et al. 2019

Appl Environ Microbiol

View full text Add to dashboard Cite

The function of small secreted proteins (SSPs) in saprotrophic fungi is, for the most part, unknown. The white-rot mushroom Pleurotus ostreatus produces considerable amounts of SSPs at the onset of secondary metabolism, during colony development, and in response to chemical compounds such as 5-hydroxymethylfurfural and aryl alcohols. Genetic manipulation of Ssp1, by knockdown (KDssp1) or overexpression (OEssp1), indicated that they are, in fact, involved in the regulation of the ligninolytic system. To elucidate their potential involvement in fungal development, quantitative secretome analysis was performed during the trophophase and the idiophase and at a transition point between the two growth phases. The mutations conferred a time shift in the secretion and expression patterns: OEssp1 preceded the entrance to idiophase and secondary metabolism, while KDssp1 was delayed. This was also correlated with expression patterns of selected genes. The KDssp1 colony aged at a slower pace, accompanied by a slower decline in biomass over time. In contrast, the OEssp1 strain exhibited severe lysis and aging of the colony at the same time point. These phenomena were accompanied by variations in yellow pigment production, characteristic of entrance of the wild type into idiophase. The pigment was produced earlier and in a larger amount in the OEssp1 strain and was absent from the KDssp1 strain. Furthermore, the dikaryon harboring OEssp1 exhibited a delay in the initiation of fruiting body formation as well as earlier aging. We propose that Ssp1 might function as a part of the fungal communication network and regulate the pattern of fungal development and metabolism in P. ostreatus. IMPORTANCE Small secreted proteins (SSPs) are common in fungal saprotrophs, but their roles remain elusive. As such, they comprise part of a gene pool which may be involved in governing fungal lifestyles not limited to symbiosis and pathogenicity, in which they are commonly referred to as "effectors." We propose that Ssp1 in the white-rot fungus Pleurotus ostreatus regulates the transition from primary to secondary metabolism, development, aging, and fruiting body initiation. Our observations uncover a novel regulatory role of effector-like SSPs in a saprotroph, suggesting that they may act in fungal communication as well as in response to environmental cues. The presence of Ssp1 homologues in other fungal species supports a common potential role in environmental sensing and fungal development.

show abstract

Section: Figsupporting

confidence: 70%