Phenomenal Properties of the Domain Ensembles in Proteins

Zubow, Kristina; Zubow, Viktor Anatolievich; Zubow, Anatolij

doi:10.21767/2471-8084.100014

Cited by 1 publication

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“…Many properties of biomaterials depend significantly on waterbiopolymer interactions. The interactions between water and biomolecules were investigated by NMR methods for a long period of time (Kuntz et al 1969;Wüthrich et al 1996;Krishman 1996;Wider 1998;Szuminska et al 2001;Van-Quynh et al 2003;Cosgrove et al 2007;Rodin et al 2014Rodin et al , 2017Belton 2011;Foster et al 2016;Mandala et al 2018;Yazawa et al 2016;Zubow et al 2016;Kerch 2018;Callaghan and Lelievre 1986). The data obtained discovered hydration features and resulted in the development of the hydration models (Kuntz et al 1969;Kuntz and Kauzmann 1974;Mathur-De Vre 1979;Wüthrich et al 1996;Otting 1997;Halle 2004;Cosgrove et al 2007;Belton 2011;Xie et al 2019).…”

Section: Introductionmentioning

confidence: 99%

NMR techniques in studying water in biotechnological systems

Rodin

2020

Biophys Rev

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Different NMR methodologies have been considered in studying water as a part of the structure of heterogeneous biosystems. The current work mostly describes NMR techniques to investigate slow translational dynamics of molecules affecting anisotropic properties of polymers and biomaterials. With these approaches, information about organized structures and their stability could be obtained in conditions when external factors affect biomolecules. Such changes might include rearrangement of macromolecular conformations at fabrication of nano-scaffolds for tissue engineering applications. The changes in water-fiber interactions could be mirrored by the magnetic resonance methods in various relaxations, double-quantum filtered (DQF), 1D and 2D translational diffusion experiments. These findings effectively demonstrate the current state of NMR studies in applying these experiments to the various systems with the anisotropic properties. For fibrous materials, it is shown how NMR correlation experiments with two gradients (orthogonal or collinear) encode diffusion coefficients in anisotropic materials and how to estimate the permeability of cell walls. It is considered how the DQF NMR technique discovers anisotropic water in natural polymers with various cross-links. The findings clarify hydration sites, dynamic properties, and binding of macromolecules discovering the role of specific states in improving scaffold characteristics in tissue engineering processes. Showing the results in developing these NMR tools, this review focuses on the ways of extracting information about biophysical properties of biomaterials from the NMR data obtained.

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Section: Introductionmentioning

confidence: 99%

NMR techniques in studying water in biotechnological systems

Rodin

2020

Biophys Rev

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Phenomenal Properties of the Domain Ensembles in Proteins

Cited by 1 publication

References 16 publications

NMR techniques in studying water in biotechnological systems

NMR techniques in studying water in biotechnological systems

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