Phenolic content of extra virgin olive oil is essential to restore endothelial dysfunction but not to prevent vascular inflammation in atherosclerotic lesions of Apo E deficient mice

Pomace Olive Oil Concentrated in Triterpenic Acids Restores Vascular Function, Glucose Tolerance and Obesity Progression in Mice

“…The descending thoracic aorta was dissected and segments next to the aortic arch were selected and placed in modified Krebs-Henseleit bicarbonate solution (KHS), as previously described [33]. Briefly, aortic rings (1.5-2 mm in length) were mounted on a wire myograph (Danish MyoTechnology, Aarhus, Denmark) filled with KHS.…”

A review on management of cardiovascular diseases by olive polyphenols

“…(2016) reported that diets enrich with OO‐protected atherosclerosis as compared to saturated fatty acid‐rich diet in niacin‐treated mice. Another in vivo study conducted by Claro, Ogalla, Rodriguez‐Rodriguez, Herrera, and de Sotomayor (2015) also claimed that EVOO polyphenols lowered the protein expression and macrophage accumulation of iNOS, VCAM‐1, ICAM‐1, NFκB, TNF‐α, and superoxide anion production. Furthermore, EVOO polyphenols improved endothelial function and lowered lipid accumulation within the atherosclerotic lesion of Apo E‐deficient mice.…”

Protective Effect of Olive Oil Microconstituents in Atherosclerosis: Emphasis on PAF Implicated Atherosclerosis Theory

“…Additionally, an intervention performed in ApoE-deficient mice fed with a high fat diet, supplemented (15%, w / w ) with either an EVOO or an EVOO containing four times higher amounts of phenolic compounds, showed that both groups equally downregulated expression of the adhesion molecules, TNFα and NF-kB, and exhibited a reduction of macrophage infiltration in a similar extent, even though only the enriched EVOO enhanced the endothelium-dependent vasodilation in aortic rings [ 86 ]. Another study with a very similar design in LDL-receptor knockout Leiden mice concluded that only EVOO protects against high fat diet-induced atherosclerosis development in the context of obesity, while the EVOO that was enriched with phenolic compounds did not present an antiatherogenic effect [ 87 ].…”