Phenol biodegradation by isolated Citrobacter strain under hypersaline conditions

Deng, Tao; Wang, Hongyu; Yang, Kai

doi:10.2166/wst.2017.543

Cited by 15 publications

(12 citation statements)

References 18 publications

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“…4 shows that the cell growth rate and CB degradation rate reached the maximum at approximately 30% of the degradation rate at NaCl concentration of 0.05 mol L À1 , which may due to the cellular water loss or cytoplasm recession under high salt concentration. 47 Correspondingly, the cell growth rate also had similar changes. Trace salinity was good for cell growth and CB degradation, whereas high salinity inhibited cell growth and CB degradation remarkably.…”

Section: Factors Affecting Cb Degradationmentioning

confidence: 71%

Superior performance and mechanism of chlorobenzene degradation by a novel bacterium

et al. 2019

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Section: Factors Affecting Cb Degradationmentioning

confidence: 71%

Superior performance and mechanism of chlorobenzene degradation by a novel bacterium

et al. 2019

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“…This phenomenon was similar to many previous studies that the removal performance of pollutants by isolated microorganisms worsened at temperatures that were too low or too high. [32][33][34] Regarding pH, Fig. 3B shows satisfactory performance when the pH was 8.0, at which 1000 mg L À1 phenol was completely degraded within 60 h at 5% NaCl.…”

Section: Effect Of Incubation Ph Temperature and Nacl Concentration mentioning

confidence: 99%

Glycine betaine enhances biodegradation of phenol in high saline environments by the halophilic strainOceanobacillussp. PT-20

et al. 2019

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The halophilic bacterial strain PT-20, isolated from saline alkali soil samples and identified as a member of the genus Oceanobacillus, exhibited a robust ability to degrade phenol under high salt conditions. It was determined that strain PT-20 was capable of degrading 1000 mg L À1 phenol completely in the presence of 10% NaCl within 120 h. Under the optimal degradation conditions, pH 8.0, 3% NaCl and 30 C, 1000 mg L À1 phenol could be completely degraded in 48 h. Interestingly, the biodegradation rate of phenol was dramatically improved in the presence of glycine betaine. When glycine betaine was added, the time required to degrade 1000 mg L À1 phenol completely was significantly reduced from 120 h to 72 h, and the corresponding average degradation rate increased from 8.43 to 14.28 mg L À1 h À1 with 10% NaCl. Furthermore, transcriptome analysis was performed to investigate the effects of phenol and glycine betaine on the transcriptional levels of strain PT-20. The results indicated that the addition of glycine betaine enhanced the resistance of cells to phenol, increased the growth rate of strain PT-20 and upregulated the expression of related enzyme genes. In addition, the results of enzyme activity assays indicated that strain PT-20 degraded phenol mainly through a meta-fission pathway.

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“…Citrobacter spp. are part of the normal flora of human and animal intestines and have been reported to degrade not only phenol, tannic acid, and lignocellulose, but also to reduce the toxicity of methyl parathion and p -nitrophenol under certain conditions (Deng et al 2018 ; Kumar et al 1999 ; Pino and Peñuela 2011 ; Wang et al 2021 ). Citrobacter spp.…”

Section: Introductionmentioning

confidence: 99%

Whole genome sequencing and analysis of fenvalerate degrading bacteria Citrobacter freundii CD-9

et al. 2022

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Citrobacter freundii CD-9 is a Gram-negative bacteria sourced from factory sludge that can use fenvalerate as its sole carbon source and has a broad degradation spectrum for pyrethroid pesticides. The whole genome of CD-9 sequenced using Illumina HiSeq PE150 was reported in this study. The CD-9 genome size was 5.33 Mb and the G + C content was 51.55%. A total of 5291 coding genes, 9 5s-rRNA, and 79 tRNA were predicted bioinformatically. 3586 genes annotated to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database that can be involved in 173 metabolic pathways, including various microbial metabolic pathways that degrade exogenous chemicals, especially those that degrade aromatic compounds, and also produce a variety of bioactive substances. Fifty genes related to pyrethroid degradation were identified in the C. freundii CD-9 genome, including 9 dioxygenase, 25 hydrolase, and 16 esterase genes. Notably, RT-qPCR results showed that from the predicted 13 genes related to fenvalerate degradation, the expression of six genes, including esterase, HAD family hydrolase, lipolytic enzyme, and gentisic acid dioxygenase, was induced in the presence of fenvalerate. In this study, the key genes and degradation mechanism of C. freundii CD-9 were analyzed and the results provide scientific evidence to support its application in environmental bioremediation. It can establish application models for different environmental pollution management by constructing genetically engineered bacteria for efficient fenvalerate or developing enzyme formulations that can be industrially produced.

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Phenol biodegradation by isolated Citrobacter strain under hypersaline conditions

Cited by 15 publications

References 18 publications

Superior performance and mechanism of chlorobenzene degradation by a novel bacterium

Superior performance and mechanism of chlorobenzene degradation by a novel bacterium

Glycine betaine enhances biodegradation of phenol in high saline environments by the halophilic strainOceanobacillussp. PT-20

Whole genome sequencing and analysis of fenvalerate degrading bacteria Citrobacter freundii CD-9

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