Microwave frequency combs (MFCs) with flexible tunability and prominent phase noise performance are of importance to many applications, including consumer electronic product, fundamental research and military defence. It is difficult for traditional electronic signal sources to meet the imperative demand in terms of high frequency scale, due to a challenging problem of deteriorating phase noise performance with increasing frequency. Photonics-assisted methods have capacity of implementing the generation of microwave signals with high frequency and low phase noise. Here we report a novel photonics-assisted MFC generation method utilizing an optoelectronic feedback loop with a Vernier configuration. The proposed MFC generation system features self-sustained oscillation, inherent multiple-mode oscillation and low phase noise level. In the proof-of-principle experiment, the MFC generation system based on a dual-path Vernier optoelectronic feedback loop is demonstrated, and the comb spacing tuning from 3.072 to 4.710 GHz and the single sideband phase noise of −99.60 dBc/Hz at 10 kHz offset from the carrier are achieved.