Pharmacological evidence for the role of nitric oxide in the modulation of stress-induced anxiety by morphine in rats

Anand, Rashmi; Gulati, Kavita; Ray, Arunabha

doi:10.1016/j.ejphar.2011.11.032

Cited by 30 publications

(21 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, the total number of closed arm entries, which is a clear index of general motor activity, was always recorded, and the differences between the number of total arm entries (open arm + closed arm) in controls, RS exposed and drug treated rats were marginal (data not shown) -indicating that locomotor activity was possibly not influencing our interpretation of anxiety modulation in the EPM. Such anxiolytic effects of morphine have also been reported earlier in other experimental models [18,20,21]. Further, biochemical analysis of brain homogenates revealed lowered levels of NOx (stable metabolites of nitric oxide) and elevated Hsp70 expression after RS exposure in comparison to controls.…”

Section: Discussionmentioning

confidence: 54%

“…Our study evaluated the effects of morphine and its cellular mechanisms during acute and chronic RS induced anxiety modulation in rats by using the EPM test. Our earlier studies have shown that NO plays a crucial role in stress regulation and that morphine induced reversal of stress effects may be mediated through NO [4,5,18]. The present study further evaluated the cellular mechanisms in the anxiolytic effects of morphine and its interactions with NO during both acute and chronic stress in rats.…”

Section: Discussionmentioning

confidence: 85%

See 1 more Smart Citation

Effects of morphine on stress induced anxiety in rats: Role of nitric oxide and Hsp70

Joshi

Ray

Gulati

2015

Physiology & Behavior

Self Cite

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 85%

Effects of morphine on stress induced anxiety in rats: Role of nitric oxide and Hsp70

Joshi

Ray

Gulati

2015

Physiology & Behavior

Self Cite

View full text Add to dashboard Cite

“…Likewise, there is pharmacological evidence that anxiolytic action of morphine (prototype of μ-opioid receptor agonist) is modulated via nitric oxide. In this regard, co-administration of Larginine and morphine induced synergistic anxiolytic effects while L-NAME, neutralized the anxiolytic effects of morphine in rats (Anand et al 2012).…”

Section: Discussionmentioning

confidence: 97%

“…There is also evidence that morphine administration stimulates the release of nitric oxide in rabbit aqueous humor (Dortch-Carnes and Russell 2007). On the other hand, several experiments have shown that relationships between the nitrergic and the opioidergic systems have been paired in many functions including peripheral antinociception induced by codeine (Ortiz et al 2005), thermoregulation (Benamar et al 2002), neuropathic pain (Hervera et al 2011) stress-induced anxiety (Anand et al 2012), seizure susceptibility (Homayoun et al 2002) dependence and withdrawal (Cuellar et al 2000).…”

Section: Introductionmentioning

confidence: 98%

Modulation of opioid-induced feeding behavior by endogenous nitric oxide in neonatal layer-type chicks

2015

View full text Add to dashboard Cite

The current study was designed to evaluate the effects of central administration of L-arginine (The precursor of nitric oxide), N(G)-nitro-L-arginine methyl ester (L-NAME), a nitric oxide (NO) synthase inhibitor, selective opioid receptor agonists and involvement of central nitrergic/opioidergic systems on feeding behavior in neonatal layer-type chicks. The results of this study showed that the intracerebroventricular (ICV) injection of L-arginine (400 and 800 nmol) significantly decreased food intake (P < 0.001) but the injection of 200 nmol L-arginine had no effect on cumulative food intake in FD3 chickens (P > 0.05). The ICV injection of L-NAME (200 and 400 nmol) increased food intake (P < 0.001) but 100 nmol of L-NAME had no significant effect (P > 0.05). On the other hand, the co-injection of 100 nmol L-NAME significantly attenuated the anorexigenic effect of 800 nmol L-arginine (P < 0.001). Moreover, the food intake of chicks was significantly decreased by ICV injection of DAMGO (μ-opioid receptor agonist, 125 pmol) (P < 0.001) while both DPDPE (δ-opioid receptor agonist, 40 pmol) and U-50488H (κ-opioid receptor agonist, 30 nmol) significantly stimulated food intake (P < 0.001). In addition, the hypophagic effect of DAMGO was significantly amplified by administration of L-arginine (P < 0.001) while the administration of L-NAME attenuated the hypophagic effect of DAMGO (P < 0.001). In contrast, co-injection of L-arginine or L-NAME with DPDPE had no effect on the hyperphagia induced by DPDPE as well as the hyperphagic effect of U-50488H on food intake was not affected by concurrent injection of L-arginine or L-NAME (P > 0.05). These results suggest that nitrergic and opioidergic systems have an important role on feeding behavior in the CNS of neonatal layer-type chicks and it seems that interaction between them is mediated by μ-opioid receptor.

show abstract

“…Chronic predictable stress (CPS) -Rats were immobilized/restrained for 1 hour/day at room temperature in specific Plexiglas restrainers (INCO, Ambala) [2,7] , for 14 days i.e. multiple sessions of same stress were employed in case of CPS.…”

Section: )mentioning

confidence: 99%

Differential effects of chronic predictable and unpredictable stress on neurobehavioral and biochemical responses in rats

Thakur¹,

Anand²,

Ray³

et al. 2015

Ther Targets Neurol Dis

View full text Add to dashboard Cite

The present study was designed to investigate the effects of exposure to chronic predictable and unpredictable stress on neurobehavioral and biochemical responses in rats. Male Wistar rats (200-250g) were exposed to either chronic predictable stress (CPS) i.e. immobilization for 1 hour/day for 14 days or chronic unpredictable stress (CUS) i.e. daily a different, random, novel stressor sequence (immobilization stress for 1h, footshock, cold stress, overnight food and water deprivation and social isolation) for 14 days. Behavioral responses were assessed by the elevated plus maze (EPM) test and biochemical parameters, viz. malondialdehyde (MDA, a marker of oxidative stress) and stable NO metabolites (NOx, marker of nitrosative stress), were measured in brain homogenates of the rats. Exposure to chronic CPS resulted in adaptation to neurobehavioral suppression in the EPM (as observed after acute Restraint stress), which was not seen after CUS. These behavioral changes after CPS and CUS were closely paralleled by alterations in the levels of brain MDA and NOx. These results suggest that CPS and CUS results in differential modulation of the neurobehavioral profile and oxidative/nitrosative stress markers in the brain. Keywords:Chronic

show abstract

Pharmacological evidence for the role of nitric oxide in the modulation of stress-induced anxiety by morphine in rats

Cited by 30 publications

References 26 publications

Effects of morphine on stress induced anxiety in rats: Role of nitric oxide and Hsp70

Effects of morphine on stress induced anxiety in rats: Role of nitric oxide and Hsp70

Modulation of opioid-induced feeding behavior by endogenous nitric oxide in neonatal layer-type chicks

Differential effects of chronic predictable and unpredictable stress on neurobehavioral and biochemical responses in rats

Contact Info

Product

Resources

About