Pharmacological evaluation and validation for the folkloric use of <i>Oligochaeta ramose</i> in constipation and diarrhea

Abstract

Crude extract of Oligochaeta ramosa and its fractions were studied to rationalize its traditional use in GIT disturbance. In spontaneous contracting jejunum preparation, O. ramosa (0.01-1.0 mg/mL) caused a transient spasmogenic effect followed by the spasmolytic effect at higher doses (3.0-10.0 mg/mL). In atropinized jejunum preparation, O. ramosa inhibit the spontaneous and K + (80 mM)-induced contraction at the similar doses (0.01-1.0 mg/mL), suggesting calcium channel blocking effect. The calcium channel blocking effect was confirmed when pretreatment of tissue with O. ramosa produced a dose-dependent shift in Ca 2+ dose-response curve to the right, similar to that produced by the verapamil. Activity-directed fractionation revealed that the spasmolytic effect is concentrated in the dichloromethane fraction while, aqueous fraction contains both spasmogenic and spasmolytic constituents. This study validate the presence of both spasmogenic and spasmolytic components mediated through muscarinic receptor activation and calcium channel blockade respectively, which may explain its traditional uses in constipation and diarrhea. Article Info

Diuretic and serum electrolyte regulation potential of aqueous methanolic extract of Solanum surattense fruit validates its folkloric use in dysuria

“…Acute toxicity test was performed by using 2, 4, 6, 8 and 10 g/kg of Ss.ME, as previously described [ 32 ].…”

Diuretic and serum electrolyte regulation potential of aqueous methanolic extract of Solanum surattense fruit validates its folkloric use in dysuria

“…Acute toxicity test was performed by using 2, 4, 6, 8 and 10 g/kg of Ss.ME, as previously described [ 32 ].…”

Phytochemical Analysis and Evaluation of the Antioxidant, Anticholinesterase, Antidiarrheal, and Antispasmodic Potential of Dichloromethane and Ethyl Acetate Fractions of Solanum surattense Burm. f

“…Pigeons were fasted overnight and allowed to drink water before the experiment was conducted. Then the pigeons were kept under observation for 24 h for the acute toxicity study [ 29 ].…”

Cyperus rotundus: A potential medicinal plant

“…Methanolic extract of C. Rotundus rhizome exhibits significant anti-cancer property against all the tested cancer cell lines [67] . C. Rotundus showed significant result in the treatment of gastrointestinal, bronchial and vascular disorder in in vivo and in vitro experimental models [68] .…”

Diuretic and serum electrolyte regulation potential of aqueous methanolic extract of Solanum surattense fruit validates its folkloric use in dysuria

“…Acute toxicity test was performed by using 2, 4, 6, 8 and 10 g/kg of Ss.ME, as previously described [ 32 ].…”

Phytochemical Analysis and Evaluation of the Antioxidant, Anticholinesterase, Antidiarrheal, and Antispasmodic Potential of Dichloromethane and Ethyl Acetate Fractions of Solanum surattense Burm. f

“…Pigeons were fasted overnight and allowed to drink water before the experiment was conducted. Then the pigeons were kept under observation for 24 h for the acute toxicity study [ 29 ].…”

Cyperus rotundus: A potential medicinal plant

“…Methanolic extract of C. Rotundus rhizome exhibits significant anti-cancer property against all the tested cancer cell lines [67] . C. Rotundus showed significant result in the treatment of gastrointestinal, bronchial and vascular disorder in in vivo and in vitro experimental models [68] .…”

Diuretic and serum electrolyte regulation potential of aqueous methanolic extract of Solanum surattense fruit validates its folkloric use in dysuria

“…Acute toxicity test was performed by using 2, 4, 6, 8 and 10 g/kg of Ss.ME, as previously described [ 32 ].…”

Phytochemical Analysis and Evaluation of the Antioxidant, Anticholinesterase, Antidiarrheal, and Antispasmodic Potential of Dichloromethane and Ethyl Acetate Fractions of Solanum surattense Burm. f

“…Pigeons were fasted overnight and allowed to drink water before the experiment was conducted. Then the pigeons were kept under observation for 24 h for the acute toxicity study [ 29 ].…”

Cyperus rotundus: A potential medicinal plant

“…Methanolic extract of C. Rotundus rhizome exhibits significant anti-cancer property against all the tested cancer cell lines [67] . C. Rotundus showed significant result in the treatment of gastrointestinal, bronchial and vascular disorder in in vivo and in vitro experimental models [68] .…”