Pharmacokinetics and Excretion Study of Lycium barbarum Polysaccharides in Rats by FITC-Fluorescence Labeling

Abstract

A high-performance gel permeation chromatography fluorescence detection (HPGPC-FD) method combined with fluorescein isothiocyanate (FITC) labeling was established for the microanalysis of L. barbarum polysaccharides (LBP). The calibration curves linear over the range of 0.2–20 µg/mL in rat plasma, and 0.25–500 μg/mL in urine and feces samples with correlation coefficients greater than 0.99. The inter-day and intra-day precisions (RSD, %) of the method were under 15% with the relative recovery ranging from 84.6% to 104.0% and the RSD ranging from 0.47% to 7.28%. The concentration–time curve of LBP-FITC in plasma following intragastric administration at 100, 50 and 25 mg/kg well fitted to a nonlinear model. LBP-FITC slowly eliminated from plasma according to the long half-lives (t1/2 = 31.39, 38.09, and 45.76 h, respectively) and mean retention times (MRT0–t = 18.38, 19.15 and 20.07 h, respectively; AUC0–∞ = 230.49, 236.18 and 242.57 h, respectively) after administration of LBP-FITC at doses of 100, 50, and 25 mg/kg, respectively. After intragastric administration at 50 mg/kg for 72 h, the concentration of LBP-FITC in urine and feces was 0.09 ± 0.04% and 92.18 ± 3.61% respectively; the excretion rate of urine was the highest in 0–4 h period and decreased continuously in 4–24 h period. The excretion rate of feces was the highest in 4–10 h, 48.28 ± 9.349% in feces within 4–10 h, and decreased rapidly in 10–24 h. The present study showed that LBP was absorbed as its prototype and most proportion of LBP was excreted from feces, indicating a long time remaining in intestine.

WITHDRAWN: Study on the pharmacokinetics of Polygonatum cyrtonema polysaccharide DPC1 through fluorescence labeling

“…2E, both C max and AUC (0→∞) exhibited a positive correlation with the dose for each dose group, showcasing linear relationships of y = 0.8268x-8.5741 (R 2 = 0.9935) and y = 1.4153x + 24.585 (R 2 = 0.9531), respectively. This suggests that DPC1 demonstrates linear pharmacokinetic characteristics in mice, and similar dose-dependent outcomes were observed in the pharmacokinetic studies of other polysaccharides [25,31,38].…”

“…Pharmacokinetic experiments were conducted following the references [25,26]. Combined with the results of the pre-tests, the high, medium, and low doses were established for oral and intraperitoneal administration.…”

A purified fraction of polysaccharides from the fruits of Lycium barbarum L. improves glucose homeostasis and intestinal barrier function in high-fat diet-fed mice

“…59,60 Moreover, recent work has pointed out that a small amount of LBPs with a low molecular weight of 4.9 kDa could enter the circulation and reach the liver. 61,62 It is widely accepted that one of the main organs where glucose uptake, gluconeogenesis and glycogen synthesis take place is the liver. 63 Therefore, to rule out the direct effects of circulating LBPs-4, further studies of pharmacokinetics and tissue distribution of LBPs-4 should be investigated.…”

Sulfated Undaria pinnatifida Polysaccharide Promotes Endocytosis of Nano-Calcium Oxalate Dihydrate by Repairing Subcellular Organelles in HK-2 Cells

“…Many reports have shown that polysaccharides can be absorbed into the intestine by oral or intragastric administration, and their effects can reach the kidneys [69][70][71]. Studies have shown that the concentration of polysaccharides in the kidney can reach 60 µg/mL or even higher in animal experiments [72].…”