Biopharm. Drug Dispos.
 1998
DOI: 10.1002/(sici)1099-081x(199810)19:7<439::aid-bdd124>3.0.co;2-b
|Get access via publisher |Summarize |Cite
|
Sign up to set email alerts

Pharmacokinetic and metabolism studies using uniformly stable isotope labeled proteins with HPLC/CRIMS detection

Blaire L. Osborn
1
,
Fred P. Abramson
2
Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
9
1
0
0

Citation Types

0
6
0
0

Year Published

2000
2000
2008
2008

Publication Types

Select...
9
1

Relationship

0
10

Authors

Journals

citations

Cited by 10 publications

(6 citation statements)
references

References 11 publications

0
6
0
0
Order By: Relevance
“…The half-life of circulating ghrelin is approximately 30 mins (26), and the half-life of circulating rat GH is approximately 10 mins (29). Thus, we conclude that the low doses of ICY ghrelin over the present 5•day protocol that increased serum GH concentration 2 hrs after the last treatment likely reflect a central action of the peptide.…”
Section: Discussionmentioning
confidence: 70%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
“…The half-life of circulating ghrelin is approximately 30 mins (26), and the half-life of circulating rat GH is approximately 10 mins (29). Thus, we conclude that the low doses of ICY ghrelin over the present 5•day protocol that increased serum GH concentration 2 hrs after the last treatment likely reflect a central action of the peptide.…”
Section: Discussionmentioning
confidence: 70%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
“…The mass spectrometry field has advanced to a stage where the administration of 1:1 mixture may not be entirely necessary for qualitative metabolite identification purposes; however, such an approach could be useful if additional quantitative information could be derived from these studies, especially if synthetic standards of metabolites are not available. One approach that has received limited publicity is the application of chemical reaction interface mass spectrometry (CRIMS) in combination with stable isotope isotope-labeled compounds − . With CRIMS, LC or GC eluents are directly combusted inside a microwave-induced plasma chamber followed by further reaction with an oxidizing gas such as sulfur dioxide .…”
Section: Application Of Stable Isotope-labeled Compounds In Clinical ...mentioning
confidence: 99%
“…The reason for uniform response is that all drug-related compounds maintaining the stable isotope labels get converted to the same neutral elements prior to mass spectral analyses. The hyphenation of liquid chromatography with CRIMS presents an opportunity to perform quantitative measurement of metabolites in the absence of authentic standards and radiolabeled compounds − . Nonetheless, there are certain drawbacks and limitations with this technology.…”
Section: Application Of Stable Isotope-labeled Compounds In Clinical ...mentioning
confidence: 99%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
“…In this context, the replacement of radioisotope (radio‐iodine‐labeled protein) by stable isotope‐labeled proteins could be an interesting methodology to investigate. This type of study was performed for assessing growth hormone (rGH) pharmacokinetics in rats by injecting intravenously, “uniformly” 13 C and 15 N‐labeled rGH (Osborn & Abramson, 1998). Labeled rGH was produced by cell culture and gave a compound with 81% labeling.…”
Section: Applications Of Lc Coupled To Molecular Msmentioning
confidence: 99%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
scite logo

Scite is an AI-powered platform that helps researchers discover and evaluate scientific literature through Smart Citations, showing whether studies support or contradict a claim. Now part of Research Solutions, Scite has indexed 1.6B+ citations, partners with 30+ publishers, and serves 2M users worldwide.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Status

Copyright © 2026 Scite LLC. All rights reserved.

Made with 💙 for researchers

Part of the Research Solutions Family.

Research Solutions Logo