Phantom encodes the 25-hydroxylase of Drosophila melanogaster and Bombyx mori: a P450 enzyme critical in ecdysone biosynthesis

Warren, James T.; Petryk, Anna; Marqués, Guillermo; Parvy, Jean-Philippe; Shinoda, Tetsuro; Itoyama, Kyo; Kobayashi, Jun; Jarcho, Michael; Li, Yutai; O’Connor, Michael B.; Dauphin‐Villemant, Chantal; Gilbert, Lawrence I.

doi:10.1016/j.ibmb.2004.06.009

Cited by 266 publications

(261 citation statements)

References 58 publications

(100 reference statements)

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“…To address this issue, we studied the expression of Phm and Dib, two cytochrome P450 enzymes specific to the prothoracic gland cells of the larval ring gland that are required for E biosynthesis (Warren et al, 2002(Warren et al, , 2004Niwa et al, 2004). By Western blot analysis, the significant, although low, expression of both proteins in these same early third instar larval samples is indicative of a rather constant capacity to synthesize E during this period (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…4) (Warren et al, 2002(Warren et al, , 2004Petryk et al, 2003;Niwa et al, 2004). Quantitative Western blot analysis, performed on the insoluble protein remaining after ecdysteroid extraction (see Experimental Procedures section), reveals significant expression throughout the third instar of both Phantom (Phm, the terminal 25-hydroxylase) and Disembodied (Dib, the subsequent 22-hydroxylase) (Fig.…”

Section: Expression Of Cyp306a1 (Phantom) and Cyp302a1 (Disembodied) mentioning

confidence: 99%

“…Both the 3-dehydro metabolites 3-dehydroecdysone (3DE) and 3-dehydro-20-hydroxyecdysone (3D20E), and their 3-␣-epimeric derivatives, 3-␣-epi-ecdysone (E') and 3-␣-epi-20-hydroxyecdysone (20E'), were obtained by chemical oxidation and reduction of the parent ecdysteroid (Spindler, et al, 1977;Dinan and Rees, 1978). Rabbit antiPhantom (Phm) peptide antibodies (Warren et al, 2004) and rabbit antiDisembodied (Dib) antibodies, similarly made from an N-terminal Dib peptide (Warren et al, 2002), were obtained from FabGennix International (Shreveport, LA).…”

Section: Chemicals and Ecdysteroid Standardsmentioning

confidence: 99%

“…Twenty wild type brain-ring gland complexes were treated the same, yielding 100 l in 1ϫ sample buffer. S2R protein samples were made by transfection of Schneider S2R cells with phantom or disembodied cDNA in pBRAcPA, using DDAB as the transfection reagent (Warren et al, 2002(Warren et al, , 2004. After 5 days, the cells were collected, suspended in 1ϫ sample buffer, boiled 5 min, and centrifuged at 14,000 rpm for 10 min.…”

Section: Quantitative Western Blotsmentioning

confidence: 99%

“…Primary antibody reactions were at 4°C overnight, i.e., 1:500 of rabbit anti-Phm peptide antibodies (Warren et al, 2004) or rabbit anti-Dib peptide antibodies, 1:1,000 rabbit anti-histone H3 (Upstate), or 1:25 mouse anti-tubulin E7 (Hybridoma Bank). Secondary antibody reactions were at room temperature for 1 hr (1:10K goat anti-mouse IRDye 700 or 800 or goat anti-rabbit IRDye 700 or 800).…”

Section: Quantitative Western Blotsmentioning

confidence: 99%

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Discrete pulses of molting hormone, 20‐hydroxyecdysone, during late larval development of Drosophila melanogaster: Correlations with changes in gene activity

Warren

Yerushalmi

Shimell

et al. 2005

Developmental Dynamics

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159

168

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Section: Discussionmentioning

confidence: 99%

Section: Expression Of Cyp306a1 (Phantom) and Cyp302a1 (Disembodied) mentioning

confidence: 99%

Section: Chemicals and Ecdysteroid Standardsmentioning

confidence: 99%

Section: Quantitative Western Blotsmentioning

confidence: 99%

Section: Quantitative Western Blotsmentioning

confidence: 99%

See 3 more Smart Citations

Discrete pulses of molting hormone, 20‐hydroxyecdysone, during late larval development of Drosophila melanogaster: Correlations with changes in gene activity

Warren

Yerushalmi

Shimell

et al. 2005

Developmental Dynamics

Self Cite

159

168

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The insect growth regulator insecticide cyromazine causes earlier emergence in Drosophila melanogaster

Wouw

Batterham

Daborn

2006

Arch Insect Biochem Physiol

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The insecticide cyromazine, classified as an insect growth regulator, inhibits the growth and development of Diptera. The precise mode of cyromazine action remains unknown. We investigated mortality and developmental time effects after exposing first instar Drosophila melanogaster larvae to cyromazine for 8-h intervals. Significant increases in mortality were only observed for cyromazine exposure within the first 16 h of the first instar stage, suggesting that cyromazine accumulates in the larvae over time and that there is a delay between cyromazine exposure and effect. We observed that exposure to cyromazine during the early first instar stage resulted in earlier eclosion of adults when compared to cyromazine exposure at later first instar stages. The presence of 20-hydroxyecdysone during cyromazine exposure significantly reduced the lethal effect of cyromazine. We raise the possibility that the mode of cyromazine action is related to the development hormone, 20-hydroxyecdysone.

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Hemolymph ecdysteroids during the last three molt cycles of the blue crab, Callinectes sapidus: quantitative and qualitative analyses and regulation

Chung

2009

Arch Insect Biochem Physiol

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The profiles of circulating ecdysteroids during the three molt cycles prior to adulthood were monitored from the juvenile blue crab, Callinectes sapidus. Ecdysteroid patterns are remarkably similar in terms of peak concentrations ranging between 210-330 ng/ml hemolymph. Analysis of hemolymph at late premolt stage revealed six different types of ecdysteroids with ponasterone A (PoA) and 20-OH ecdysone (20-OH E) as the major forms. This ecdysteroid profile was consistent in all three molt cycles. Bilateral eyestalk ablation (EA) is a procedure that removes inhibitory neurohormones including crustacean hyperglycemic hormone (CHH) and molt-inhibiting hormone (MIH) and often results in precocious molting in crustaceans. However, the inhibitory roles of these neuropeptides in vivo have not yet been tested in C. sapidus. We determined the regulatory roles of CHH and MIH in the circulating ecdysteroid from ablated animals through daily injection. A daily administration of purified native CHH and MIH at physiological concentration maintained intermolt levels of ecdysteroids in the EA animals. This suggests that Y organs (YO) require a brief exposure to CHH and MIH in order to maintain the low level of ecdysteroids. Compared to intact animals, the EA crabs did not exhibit the level of peak ecdysteroids, and the major ecdysteroid turned out to be 20-OH E, not PoA. These results further underscore the important actions of MIH and CHH in ecdysteroidogenesis, as they not only inhibit, but also control the composition of output of the YO activity.

show abstract

Phantom encodes the 25-hydroxylase of Drosophila melanogaster and Bombyx mori: a P450 enzyme critical in ecdysone biosynthesis

Cited by 266 publications

References 58 publications

Discrete pulses of molting hormone, 20‐hydroxyecdysone, during late larval development of Drosophila melanogaster: Correlations with changes in gene activity

Discrete pulses of molting hormone, 20‐hydroxyecdysone, during late larval development of Drosophila melanogaster: Correlations with changes in gene activity

The insect growth regulator insecticide cyromazine causes earlier emergence in Drosophila melanogaster

Hemolymph ecdysteroids during the last three molt cycles of the blue crab, Callinectes sapidus: quantitative and qualitative analyses and regulation

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