Phage display—A powerful technique for immunotherapy

Bazan, Justyna; Całkosiński, Ireneusz; Gamian, Andrzej

doi:10.4161/hv.21704

Cited by 36 publications

(30 citation statements)

References 107 publications

(116 reference statements)

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“…The displayed recombinant peptides represent inexpensive synthesis methodology compared with the production of antibodies or proteins, making their cost‐effective production easy to implement in large‐scale industrial processes . Recombinant antibodies can be used as diagnostic tools or as prophylactic or therapeutic vaccination agents . In addition, bacteriophages have some marked advantages for the development of vaccine delivery systems: they are highly stable in different environmental conditions and the associated production cost are low and the small size of peptides increases their penetrating capacity, a pivotal question for therapeutics targeting.…”

Section: Phage Displaymentioning

confidence: 99%

Back to the future: bacteriophages as promising therapeutic tools

Domingo‐Calap

Georgel

Bahram

2016

HLA

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Bacteriophages (phages), natural predators of bacteria, are becoming increasingly attractive in medical and pharmaceutical applications. After their discovery almost a century ago, they have been particularly instrumental in the comprehension of basic molecular biology and genetics processes. The more recent emergence of multi-drug-resistant bacteria requires novel therapeutic strategies, and phages are being (re)considered as promising potential antibacterial tools. Furthermore, phages are also used for other purposes, e.g. vaccine production, gene/drug carriers, bacterial detection and typing. These new alternative approaches using phages are of major interest and have allowed unexpected developments, from the decipherment of fundamental biological processes to potential clinical applications.

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Section: Phage Displaymentioning

confidence: 99%

Back to the future: bacteriophages as promising therapeutic tools

Domingo‐Calap

Georgel

Bahram

2016

HLA

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“…In addition to the bacteriophage therapy, phages can also be used in clinical diagnostics (Schofield, Sharp, & Westwater, ), applied as vehicles for vaccines delivery or as potential carriers of therapeutic genes (Haq, Chaudhry, Akhtar, Andleeb, & Qadri, ). Also phage display, as a technique for the study of protein‐protein, protein‐peptide and protein‐DNA interactions, is possible thanks to existence of phages (Bazan, Całkosiński, & Gamian, ). Due to versatility of applications and consequently potential higher phage production needs in the future, it is important to understand how physiological state of the host influence propagation of phages.…”

Section: Introductionmentioning

confidence: 99%

Effect of bacterial growth rate on bacteriophage population growth rate

2017

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It is important to understand how physiological state of the host influence propagation of bacteriophages (phages), due to the potential higher phage production needs in the future. In our study, we tried to elucidate the effect of bacterial growth rate on adsorption constant (δ), latent period (L), burst size (b), and bacteriophage population growth rate (λ). As a model system, a well‐studied phage T4 and Escherichia coli K‐12 as a host was used. Bacteria were grown in a continuous culture operating at dilution rates in the range between 0.06 and 0.98 hr−1. It was found that the burst size increases linearly from 8 PFU·cell−1 to 89 PFU·cell−1 with increase in bacteria growth rate. On the other hand, adsorption constant and latent period were both decreasing from 2.6∙10‐9 ml·min−1 and 80 min to reach limiting values of 0.5 × 10‐9 ml·min−1 and 27 min at higher growth rates, respectively. Both trends were mathematically described with Michaelis–Menten based type of equation and reasons for such form are discussed. By applying selected equations, a mathematical equation for prediction of bacteriophage population growth rate as a function of dilution rate was derived, reaching values around 8 hr−1 at highest dilution rate. Interestingly, almost identical description can be obtained using much simpler Monod type equation and possible reasons for this finding are discussed.

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“…Although vaccine administration through subcutaneous or intramuscular injection is more widespread than other administration routes, phage-based vaccines are stable in the gastrointestinal tract and thus represent potential to be used orally [ 62 ]. Neverthesless, there are some speculations on the risk of oral administration because phages can infect the gut microbiota and perturb the microbial flora of the body.…”

Section: Bacteriophages As Promising Tools For Vaccine Developmentmentioning

confidence: 99%

Phage display as a promising approach for vaccine development

et al. 2016

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Bacteriophages are specific antagonists to bacterial hosts. These viral entities have attracted growing interest as optimal vaccine delivery vehicles. Phages are well-matched for vaccine design due to being highly stable under harsh environmental conditions, simple and inexpensive large scale production, and potent adjuvant capacities. Phage vaccines have efficient immunostimulatory effects and present a high safety profile because these viruses have made a constant relationship with the mammalian body during a long-standing evolutionary period. The birth of phage display technology has been a turning point in the development of phage-based vaccines. Phage display vaccines are made by expressing multiple copies of an antigen on the surface of immunogenic phage particles, thereby eliciting a powerful and effective immune response. Also, the ability to produce combinatorial peptide libraries with a highly diverse pool of randomized ligands has transformed phage display into a straightforward, versatile and high throughput screening methodology for the identification of potential vaccine candidates against different diseases in particular microbial infections. These libraries can be conveniently screened through an affinity selection-based strategy called biopanning against a wide variety of targets for the selection of mimotopes with high antigenicity and immunogenicity. Also, they can be panned against the antiserum of convalescent individuals to recognize novel peptidomimetics of pathogen-related epitopes. Phage display has represented enormous promise for finding new strategies of vaccine discovery and production and current breakthroughs promise a brilliant future for the development of different phage-based vaccine platforms.

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Phage display—A powerful technique for immunotherapy

Cited by 36 publications

References 107 publications

Back to the future: bacteriophages as promising therapeutic tools

Back to the future: bacteriophages as promising therapeutic tools

Effect of bacterial growth rate on bacteriophage population growth rate

Phage display as a promising approach for vaccine development

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