pH responsive poly amino-acid hydrogels formed via silk sericin templating

Kurland, Nicholas E.; Ragland, Robert B.; Zhang, Aolin; Moustafa, M. E.; Kundu, Subhas C.; Yadavalli, Vamsi K.

doi:10.1016/j.ijbiomac.2014.07.036

Cited by 21 publications

(14 citation statements)

References 47 publications

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“…The release kinetics of the loaded drug molecules depend significantly on the swelling degree of the polymer matrix [13][14][15][16]. Recently, smart hydrogels showing strong and abrupt responses (changes in their swelling degree) to small changes of the environmental conditions have been the subject of extensive research [17][18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Fully amino acid-based hydrogel as potential scaffold for cell culturing and drug delivery

Juriga¹,

Sipos²,

Hegedűs³

et al. 2019

Beilstein J. Nanotechnol.

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Polymer hydrogels are ideal scaffolds for both tissue engineering and drug delivery. A great advantage of poly(amino acid)-based hydrogels is their high similarity to natural proteins. However, their expensive and complicated synthesis often limits their application. The use of poly(aspartic acid) (PASP) seems an appropriate solution for this problem due to the relatively cheap and simple synthesis of PASP. Using amino acids not only as building blocks in the polymer backbone but also as cross-linkers can improve the biocompatibility and the biodegradability of the hydrogel. In this paper, PASP cross-linked with cystamine (CYS) and lysine-methylester (LYS) was introduced as fully amino acid-based polymer hydrogel. Gels were synthesized employing six different ratios of CYS and LYS. The pH dependent swelling degree and the concentration of the elastically active chain were determined. After reduction of the disulfide bonds of CYS, the presence of thiol side groups was also detected. To determine the concentration of the reactive cross-linkers in the hydrogels, a new method based on the examination of the swelling behavior was established. Using metoprolol as a model drug, cell proliferation and drug release kinetics were studied at different LYS contents and in the presence of thiol groups. The optimal ratio of cross-linkers for the proliferation of periodontal ligament cells was found to be 60−80% LYS and 20−40% CYS. The reductive conditions resulted in an increased drug release due to the cleavage of disulfide bridges in the hydrogels. Consequently, these hydrogels provide new possibilities in the fields of both tissue engineering and controlled drug delivery.

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Section: Introductionmentioning

confidence: 99%

Fully amino acid-based hydrogel as potential scaffold for cell culturing and drug delivery

Juriga¹,

Sipos²,

Hegedűs³

et al. 2019

Beilstein J. Nanotechnol.

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“…In the field of regenerative medicine, owing to its hydrophilic and biodegradable characteristics, sericin is mostly copolymerized or blended with other polymers to form various scaffolds in order to help obtain improved properties for biomedical applications 20 21 22 23 24 25 . Although sericin reportedly formed several semi-IPNs with other polymers including polyacrylamide and ploy(aspartic acid) 26 27 28 where sericin was often blended with a crosslinked polymer network 26 27 28 , the use of sericin with biopolymers to generate a full IPN hydrogel with double networks has never been explored. That is because in their studies sericin is difficult to be crosslinked on its own as it was largely degraded due to the conventional extraction process (involving high heat and alkaline), thus becoming amorphous with low molecular weights 29 .…”

mentioning

confidence: 99%

Design and performance of a sericin-alginate interpenetrating network hydrogel for cell and drug delivery

Zhang

Liu

Huang

et al. 2015

Sci Rep

115

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Although alginate hydrogels have been extensively studied for tissue engineering applications, their utilization is limited by poor mechanical strength, rapid drug release, and a lack of cell adhesive ability. Aiming to improve these properties, we employ the interpenetrating hydrogel design rationale. Using alginate and sericin (a natural protein with many unique properties and a major component of silkworm silk), we develop an interpenetrating polymer network (IPN) hydrogel comprising interwoven sericin and alginate double networks. By adjusting the sericin-to-alginate ratios, IPNs’ mechanical strength can be adjusted to meet stiffness requirements for various tissue repairs. The IPNs with high sericin content show increased stability during degradation, avoiding pure alginate’s early collapse. These IPNs have high swelling ratios, benefiting various applications such as drug delivery. The IPNs sustain controlled drug release with the adjustable rates. Furthermore, these IPNs are adhesive to cells, supporting cell proliferation, long-term survival and migration. Notably, the IPNs inherit sericin’s photoluminescent property, enabling bioimaging in vivo. Together, our study indicates that the sericin-alginate IPN hydrogels may serve as a versatile platform for delivering cells and drugs, and suggests that sericin may be a building block broadly applicable for generating IPN networks with other biomaterials for diverse tissue engineering applications.

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“…Sericin by cross-linking and mixing with other polymers can form a desirable scaffold with application in the pharmaceutical and cosmetic preparations (Aramwit et al, 2012). No nutritional effect Reduce blood glucose and glycosylated hemoglobin (HbA1c) levels, improve glucose tolerance and increase insulin levels Increase pancreatic β cell mass Increase serum levels of the insulin-like growth factor-1 (IGF-1), growth hormone (GH) receptor, and adiponectin levels Decrease leptin and resistin levels Reduce the body fat content, triglyceride, and total cholesterol levels, atherogenic index, free unsaturated fats, phospholipids, very low-density lipoproteins (VLDL), low-density lipoprotein (LDL), and free fatty acid level Fibroin and Sericin Chen et al, 2013Do et al, 2012Okazaki et al, 2010Rattana et al, 2017Seo et al, 2011 Cardioprotection Protect against atherosclerosis Protect against doxorubicin (Dox)-induced cardiotoxicity Prevent isoprenaline-induced myocardial damage and hypertrophy Improve cardiac functional recovery after myocardial infarction Aramwit et al, 2009Chlapanidas et al, 2013Keawkorn et al, 2012Promphet et al, 2014Sasaki et al, 2000Zhaorigetu et al, 2001 Vascular and circulation effects Anticoagulant Vasodilation and relaxation of the smooth muscle of the artery wall Sericin Onsa-Ard et al, Tamada et al, 2004 Tissue engineering Act as chief ingredient for bioimaging, drug delivery, tissue regeneration, and scaffold for tissue regeneration Sericin and Fibroin Aramwit et al, 2015Kurland et al, 2014Panico et al, 2018Xiong et al, 2017Zhang et al, 2015 Wound healing Improve skin repair and collagen production Increase proliferation and migration of keratinocytes and fibroblasts into the injured area Sericin Aramwit and Sangcakul, 2007Aramwit et al, 2010bLiang et al, 2007 Cosmetic effects…”

Section: Silk Proteinsmentioning

confidence: 99%

Identification and applications of neuroactive silk proteins: a narrative review

Mohammadi¹,

Sadigh‐Eteghad²,

Torbati³

et al. 2019

J Appl Biomed

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In traditional medicine, natural silk is regarded as a cognitive enhancer and a cure for ameliorating the symptoms of heart disease, atherosclerosis, and metabolic disorders. In this review, general characteristics of both silk proteins, fibroin and sericin, extracted from silkworm Bombyx mori and their potential use in the neuronal disorders was discussed. Evidence shows that silk proteins exhibit neuroprotective effects in models of neurotoxicity. The antioxidant, neuroprotective, and acetylcholinesterase inhibitory mechanisms of silk proteins could prove promising in the treatment of neurodegenerative diseases. Owing to their excellent neurocompatibility and physicochemical properties, silk proteins have been used as scaffolds and drug delivery materials in the neuronal tissue engineering. These data support the potential of silk proteins as an effective complementary agent for central and peripheral neurological disorders.

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pH responsive poly amino-acid hydrogels formed via silk sericin templating

Cited by 21 publications

References 47 publications

Fully amino acid-based hydrogel as potential scaffold for cell culturing and drug delivery

Fully amino acid-based hydrogel as potential scaffold for cell culturing and drug delivery

Design and performance of a sericin-alginate interpenetrating network hydrogel for cell and drug delivery

Identification and applications of neuroactive silk proteins: a narrative review

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