PGRP-LB homolog acts as a negative modulator of immunity in maintaining the gut-microbe symbiosis of red palm weevil, Rhynchophorus ferrugineus Olivier

Dawadi, Bishnu; Wang, Xinghong; Xiao, Rong; Muhammad, Aliyu Idris; Hou, Youming; Shi, Zhanghong

doi:10.1016/j.dci.2018.04.021

Cited by 32 publications

(45 citation statements)

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“…The red palm weevil Rhynchophorus ferrugineus (Coleoptera: Curculionidae) is a devastating pest, which has been spread to various regions with palm trees in southern China, causing serious damage to the palm industry and landscape (Hou et al, 2011;Wang et al, 2015Wang et al, , 2017Muhammad et al, 2017;Ali et al, 2018). Red palm weevil larvae have a long life span, strong ability to drill collar, and strong adaptability to different environments (Shi et al, 2014;Peng et al, 2016;Dawadi et al, 2018;Habineza et al, 2019;Xiao et al, 2019). Due to damage to vascular tissues and consumption of large amounts of crown tissues, red palm weevil larvae can cause the death of palm trees (Butera et al, 2012;Muhammad et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Neuropeptides and G-Protein Coupled Receptors (GPCRs) in the Red Palm Weevil Rhynchophorus ferrugineus Olivier (Coleoptera: Dryophthoridae)

et al. 2020

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The red palm weevil Rhynchophorus ferrugineus is a devastating, invasive pest that causes serious damages to palm trees, and its invasiveness depends on its strong ability of physiological and behavioral adaptability. Neuropeptides and their receptors regulate physiology and behavior of insects, but these protein partners have not been identified from many insects. Here, we systematically identified neuropeptide precursors and the corresponding receptors in the red palm weevil, and analyzed their tissue expression patterns under control conditions and after pathogen infection. A total of 43 putative neuropeptide precursors were identified, including an extra myosuppressin peptide was identified with amino acid substitutions at two conserved sites. Forty-four putative neuropeptide receptors belonging to three classes were also identified, in which neuropeptide F receptors and insulin receptors were expanded compared to those in other insects. Based on qRT-PCR analyses, genes coding for several neuropeptide precursors and receptors were highly expressed in tissues other than the nervous system, suggesting that these neuropeptides and receptors play other roles in addition to neuro-reception. Some of the neuropeptides and receptors, like the tachykininrelated peptide and receptor, were significantly induced by pathogen infection, especially sensitive to Bacillus thuringiensis and Metarhizium anisopliae. Systemic identification and initial characterization of neuropeptides and their receptors in the red palm weevil provide a framework for further studies to reveal the functions of these ligand-and receptor-couples in regulating physiology, behavior, and immunity in this important insect pest species.

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Section: Introductionmentioning

confidence: 99%

Neuropeptides and G-Protein Coupled Receptors (GPCRs) in the Red Palm Weevil Rhynchophorus ferrugineus Olivier (Coleoptera: Dryophthoridae)

et al. 2020

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“…The reasons underlying such an intriguing pattern remain undetermined, although several hypotheses have been proposed to explain low microbial diversity in insects. One hypothesis suggests that the insect immune system fine-tunes the bacterial microbiota composition in order to tolerate only beneficial bacteria as has been seen in D. melanogaster and the red palm weevil (Lhocine et al, 2008;Ryu et al, 2008;Chandler et al, 2011;Login et al, 2011;Dawadi et al, 2018). Another hypothesis, although not exclusive, suggests that low microbial diversity results from negative interactions between co-inhabiting bacteria as has been seen between Buchnera and Rickettsia in the pea aphid (Sakurai et al, 2005), between Spiroplasma and Wolbachia in D. melanogaster (Goto et al, 2006), and between Bartonella and Rickettsia in fleas from the genus Oropsylla (Jones et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

The bacterial community associated with adult vine weevil (Otiorhynchus sulcatus) in UK populations growing on strawberry is dominated by Candidatus Nardonella

Morera‐Margarit

Bulgarelli

Pope

et al. 2019

Entomologia Exp Applicata

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Otiorhynchus sulcatus (Fabricius) (Coleoptera: Curculionidae), commonly known as black vine weevil or simply vine weevil, is an important pest of soft fruit and ornamental crops. This species is endemic to temperate areas of Europe but has spread to many other areas over the last century, including North America and Australasia. The ability of vine weevils to adapt to such different environments is difficult to reconcile with the parthenogenetic reproduction strategy, which is likely to underpin a low genetic diversity. It is therefore tempting to hypothesize that weevil adaptation to different environments is mediated, at least partly, by the microbial communities inhabiting these insects. As a first step towards testing this hypothesis we characterized the composition of the bacterial microbiota in weevils from populations feeding on strawberry plants across four geographically separate locations in the UK. We performed 16S rRNA gene Illumina amplicon sequencing, generating 2 882 853 high‐quality reads. Ecological indices, namely Chao1 and Shannon, revealed that the populations used for this study harboured a low diversity and an uneven bacterial microbiota. Furthermore, β‐diversity analysis failed to identify a clear association between microbiota composition and location. Notably, a single operational taxonomic unit phylogenetically related to Candidatus Nardonella accounted for 81% of the total sequencing reads for all tested insects. Our results indicate that vine weevil bacterial microbiota resembles that of other insects as it has low diversity and it is dominated by few taxa. A prediction of this observation is that location per se may not be a determinant of the microbiota inhabiting weevil populations. Rather, other or additional selective pressures, such as the plant species used as a food source, ultimately shape the weevil bacterial microbiota. Our results will serve as a reference framework to investigate other or additional hypotheses aimed at elucidating vine weevil adaptation to its environment.

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“…Each sample contained at least 3 larvae. Real-time quantitative PCR (RT-qPCR) was performed using FastStart Universal SYBR Green Master supplemented with Rox (Roche, Basel, Switzerland) with a 20-µL reaction volume according to a previously described protocol [29]. The no-RT control was also prepared with 5× TransScript ® All-in-One No-RT Control SuperMix (Beijing TransGen Biotech Co., Ltd., Beijing, China) following the manufacturer's instruction.…”

Section: Evaluation Of Gene Expression Profiles By Real-time Quantitamentioning

confidence: 99%

“…The red palm weevil (RPW), Rhynchophorus ferrugineus (Coleoptera: Curculionidae), is native to India and is the most notorious palm trunk-boring pest worldwide [27]. In China, RPW, as an invasive pest, has killed approximately 20,000 coconut trees, with an infestation area over 10,000 km 2 , and presents serious threats to the green ecological safety of coastal areas [28,29]. Although the effective biocontrol of RPW by pathogenic microbes has been validated under laboratory conditions [30,31], successful colonization by this species is partly due to strong individual immunity against exogenous substances [4].…”

Section: Introductionmentioning

confidence: 99%

Identification of Novel ARSB Genes Necessary for p-Benzoquinone Biosynthesis in the Larval Oral Secretion Participating in External Immune Defense in the Red Palm Weevil

Zhang

et al. 2020

IJMS

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External secretions, composed of a variety of chemical components, are among the most important traits that endow insects with the ability to defend themselves against predators, parasites, or other adversities, especially pathogens. Thus, these exudates play a crucial role in external immunity. Red palm weevil larvae are prolific in this regard, producing large quantities of p-benzoquinone, which is present in their oral secretion. Benzoquinone with antimicrobial activity has been proven to be an active ingredient and key factor for external immunity in a previous study. To obtain a better understanding of the genetic and molecular basis of external immune secretions, we identify genes necessary for p-benzoquinone synthesis. Three novel ARSB genes, namely, RfARSB-0311, RfARSB-11581, and RfARSB-14322, are screened, isolated, and molecularly characterized on the basis of transcriptome data. To determine whether these genes are highly and specifically expressed in the secretory gland, we perform tissue/organ-specific expression profile analysis. The functions of these genes are further determined by examining the antimicrobial activity of the secretions and quantification of p-benzoquinone after RNAi. All the results reveal that the ARSB gene family can regulate the secretory volume of p-benzoquinone by participating in the biosynthesis of quinones, thus altering the host’s external immune inhibitory efficiency.

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PGRP-LB homolog acts as a negative modulator of immunity in maintaining the gut-microbe symbiosis of red palm weevil, Rhynchophorus ferrugineus Olivier

Cited by 32 publications

References 70 publications

Neuropeptides and G-Protein Coupled Receptors (GPCRs) in the Red Palm Weevil Rhynchophorus ferrugineus Olivier (Coleoptera: Dryophthoridae)

Neuropeptides and G-Protein Coupled Receptors (GPCRs) in the Red Palm Weevil Rhynchophorus ferrugineus Olivier (Coleoptera: Dryophthoridae)

The bacterial community associated with adult vine weevil (Otiorhynchus sulcatus) in UK populations growing on strawberry is dominated by Candidatus Nardonella

Identification of Novel ARSB Genes Necessary for p-Benzoquinone Biosynthesis in the Larval Oral Secretion Participating in External Immune Defense in the Red Palm Weevil

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