PFOS elicits transcriptional responses of the ER, AHR and PPAR pathways in Oryzias melastigma in a stage-specific manner

Fang, Chao; Wu, Xinlong; Huang, Qiansheng; Liao, Yulin; Liu, Liangpo; Qiu, Ling; Shen, Heqing; Dong, Shuang

doi:10.1016/j.aquatox.2011.10.009

Cited by 78 publications

(39 citation statements)

References 51 publications

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“…Reproduction and the endocrine system of fish might be susceptible towards both endocrine disrupting chemicals (EDCs) (Arcand-Hoy and Benson, 1998), multiple climatic and environmental stressors (Baroiller and D'Cotta, 2001;Schreck et al, 2001). PFOS has previously been found to affect endocrine parameters, sexual development and reproduction in fish (Ankley et al, 2005;Fang et al, 2012;Mortensen et al, 2011;Oakes et al, 2005;Wang et al, 2011). However, to our knowledge there are no studies that have examined in fish how elevated pCO2 might modulate the response to PFOS exposure on hormonal and biotransformation systems.…”

Section: Introductionmentioning

confidence: 74%

Effects of elevated dissolved carbon dioxide and perfluorooctane sulfonic acid, given singly and in combination, on steroidogenic and biotransformation pathways of Atlantic cod

et al. 2014

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In the aquatic environments, the predicted changes in water temperature, pO2 and pCO2 could result in hypercapnic and hypoxic conditions in aquatic animals. These conditions are thought to affect several basic cellular and physiological mechanisms. Yet, possible adverse effects of elevated CO2 (hypercapnia) on teleost fish, as well as combined effects with emerging and legacy environmental contaminants are poorly investigated. In this study, juvenile Atlantic cod (Gadus morhua) were divided into groups and exposed to three different water bath PFOS exposure regimes (0 (control), 100 and 200 µg/L) for 5 days at 1hr/day, followed by three different CO2-levels (normocapnia, moderate (0.3%) and high (0.9%)). The moderate CO2 level is the predicted near future (within year 2300) level, while 0.9% represent severe hypercapnia. Tissue samples were collected at 3, 6 and 9 days after initiated CO2 exposure. Effects on the endocrine and biotransformation systems were examined by analyzing levels of sex steroid hormones (E2, T, 11-KT) and transcript expression of estrogen responsive genes (ERα, Vtg-α, Vtg-β, ZP2 and ZP3). In addition, transcripts for xenobiotic metabolizing enzymes (cyp1a and cyp3a) and hypoxiainducible factor (HIF-1α) were analyzed. Hypercapnia alone produced increased levels of sex steroid hormones (E2, T, 11-KT) with concomitant increase of estrogen responsive genes, while PFOS produced weak and time-dependent effects on E2-inducible gene transcription. Combined PFOS and hypercapnia exposure produced increased effects on sex steroid levels as observed for hypercapnia alone, with transcript expression patterns that are indicative of time-dependent interactive effects. Exposure to hypercapnia singly or in combination with PFOS produced modulations of the biotransformation and hypoxic responses that were apparently concentration and time-dependent. Loading plots of principal component analysis (PCA) produced a significant grouping of individual scores according to the exposure scenarios at day 6 and 9. Overall, the PCA analysis produced a unique clustering of variables that signifies a positive correlation between exposure to high PFOS concentration and expression of E2 responsive genes. Notably, this pattern was not evident for individuals exposed to PFOS concentrations in combination with elevated CO2 scenarios. To our knowledge, the present study is the first of its kind, to evaluate such effects using combined exposure to a perfluoroalkyl sulfonate and elevated levels of CO2 saturation, representative of future oceanic climate change, in any fish species or lower vertebrate.

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Section: Introductionmentioning

confidence: 74%

Effects of elevated dissolved carbon dioxide and perfluorooctane sulfonic acid, given singly and in combination, on steroidogenic and biotransformation pathways of Atlantic cod

et al. 2014

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“…PFOS elicits a transcriptional response of the PPAR pathway in Oryzias melastigma in a stage-specific manner. PPARα and PPARβ are first inhibited at 4 dpf and are induced at 10 dpf (Fang et al, 2012). The molecular mechanism underlying the variable expression pattern is unknown.…”

Section: Discussionmentioning

confidence: 99%

Induction of oxidative stress and related transcriptional effects of perfluorononanoic acid using an in vivo assessment

Yang

Liu²,

Ren

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…Biotransformation of xenobiotics in fish liver is generally associated with the CYP1, 2 and 3 families (Monostory et al 1996). PFOSA is metabolized in fish liver producing PFOS, most likely by CYP activity (Tomy et al 2003), and marine medaka embryos exposed to PFOS showed a time-dependent induction of arnt and cyp1a transcription (Fang et al 2012). Herein, we observed time and exposure related changes of cyp1a, cyp3a and pxr mRNA, albeit PFOSA alone generally did not produce any change.…”

Section: Biotransformation Responsesmentioning

confidence: 99%

Endocrine, biotransformation, and oxidative stress responses in salmon hepatocytes exposed to chemically induced hypoxia and perfluorooctane sulfonamide (PFOSA), given singly or in combination

Olufsen

Arukwe

2014

Environ Sci Pollut Res

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Abstract. The effects of hypoxia and PFOSA, given singly and also in combination on endocrine, biotransformation and oxidative stress responses were investigated in primary culture of salmon hepatocytes. Hypoxia was induced chemically using cobalt chloride (CoCl 2 ) or deferroxamine (DFO). Primary culture of salmon hepatocytes were exposed to either CoCl 2 (150 µM) or DFO (100 µM), in the presence or absence of PFOSA at 0, 25 and 50 µM for 24 and 48 h. Changes in transcript levels were analysed by quantitative (real-time) PCR using gene-specific primers. CYP, catalase, GST and SOD activities were analyzed spectrophotometrically. The hif-1α mRNA was used to validate cellular hypoxic condition, showing significantly induced transcription after 48 h exposure to DFO and CoCl 2 . Our data show that transcript levels for endocrine (ERα, Vtg and Zrp), biotransformation (cyp1a, cyp3a, gst and udpgt) and oxidative stress responses (catalase (cat), glutathione peroxidase (gpx) and glutathione reductase (gr)) were differentially modulated by PFOSA and hypoxia alone, and these effects were dependent on the response parameters and time of exposure. In combined exposure scenarios, the observed effects were apparently hypoxiadependent. However, the observed effects at transcript levels were not concomitant with those at functional protein levels, further emphasizing the potential differences that may exist between these biological levels. Biplot of principal component analysis (PCA) showed grouping of response variables after 48 h of exposure. The distribution of observations and variables indicate that PFOSA had little effect on most response variables, while clustering show a unique association between a given hypoxia condition (i.e. CoCl 2 or DFO) in combination with PFOSA and transcripts, proteins or enzyme activities.

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PFOS elicits transcriptional responses of the ER, AHR and PPAR pathways in Oryzias melastigma in a stage-specific manner

Cited by 78 publications

References 51 publications

Effects of elevated dissolved carbon dioxide and perfluorooctane sulfonic acid, given singly and in combination, on steroidogenic and biotransformation pathways of Atlantic cod

Effects of elevated dissolved carbon dioxide and perfluorooctane sulfonic acid, given singly and in combination, on steroidogenic and biotransformation pathways of Atlantic cod

Induction of oxidative stress and related transcriptional effects of perfluorononanoic acid using an in vivo assessment

Endocrine, biotransformation, and oxidative stress responses in salmon hepatocytes exposed to chemically induced hypoxia and perfluorooctane sulfonamide (PFOSA), given singly or in combination

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