Pesticide Mixture Induced DNA Damage in Peripheral Blood Erythrocytes of Freshwater Fish, Oreochromis niloticus

Ambreen, Faiza; Javed, Muhammad Tariq

doi:10.17582/journal.pjz/2018.50.1.339.346

Cited by 12 publications

(7 citation statements)

References 12 publications

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“…The slower rate of DNA repair in fishes compared to mammals make them important sentinel species in assessing environmental genotoxicity ( Espina and Weis, 1995 ) but the general similarity of toxic agents in vertebrates, assessing genotoxicity in fishes presents an opportunity of establishing risk potentials in higher vertebrates. The findings from the present study and available literature ( Ullah et al., 2016 ; Ambreen and Javed, 2018 ; D'Costa et al., 2018 ) point to the oxidative stress potentials of the pesticides, given that oxidation is a major precursor for DNA damage. It also raises concerns about the mutagenicity of pesticides not just on the fishes but to humans as well for which several investigators have determine the effects of acute and chronic pesticide exposures ( Benedetti et al., 2014 ).…”

Section: Discussionsupporting

confidence: 59%

Comparative assessment of the acute toxicity, haematological and genotoxic effects of ten commonly used pesticides on the African Catfish, Clarias gariepinus Burchell 1822

Amaeze

Komolafe

Salako

et al. 2020

Heliyon

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Freshwater fishes are faced with increasing threats due to intensification of agriculture. This study evaluated the haematological and genotoxic effects of exposure of the African Catfish, Clarias gariepinus to sublethal concentrations of commonly used pesticides in agricultural settings. The evaluated pesticides were abamectin, carbofuran, chlorpyrifos, cypermethrin, deltamethrin, dichlorvos, dimethoate, fipronil, lambda-cyhalothrin and paraquat. The fishes were initially exposed singly to the pesticides for 96 h periods to determine their LC 50 , followed by exposure to sublethal concentrations (1/100 th 96 h LC 50 ) over a 21 d period. In all cases, a control experiment with catfishes kept in dechlorinated municipal water was monitored simultaneously. The 96 h LC 50 values was found to vary widely from 2.043 μgL -1 (Lambda-cyhalothrin) to 10284.288 μgL -1 (Paraquat). Significant differences (P < 0.05) were observed between mean haematological parameters-WBC, RBC, HGB, HCT, MCH and MCHC in the exposed and control catfishes. More so, micronucleus and nuclear abnormalities occurred at significantly higher proportions in pesticide exposed catfishes. Holistic cradle to grave approach as well as fate analysis is required to mitigate the potential harmful effects of pesticides to fresh water fishes.

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Section: Discussionsupporting

confidence: 59%

Comparative assessment of the acute toxicity, haematological and genotoxic effects of ten commonly used pesticides on the African Catfish, Clarias gariepinus Burchell 1822

Amaeze

Komolafe

Salako

et al. 2020

Heliyon

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“…The nuclear and morphological alterations in RBCs of bighead carp like condensed nuclei, micronuclei, pear shape erythrocyte, and spherocyte could be due to the toxic effects of herbicides on hematopoietic tissues. Previous studies have recorded the occurrence of nuclear and morphological alterations in erythrocytes of humans [ 83 , 84 ], birds [ 7 , 9 ], and fish [ 21 , 24 , 25 , 63 , 85 ] might be related to mitochondrial damage.…”

Section: Discussionmentioning

confidence: 99%

[Retracted] Acetochlor Affects Bighead Carp (Aristichthys Nobilis) by Producing Oxidative Stress, Lowering Tissue Proteins, and Inducing Genotoxicity

Mahmood

Hussain

Ghaffar

et al. 2022

BioMed Research International

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Acetochlor is persistently used in the agroproduction sector to control broadleaf weeds. Due to frequent and continuous applications, this herbicide can reach nearby water bodies and may induce deleterious changes in aquatic life. Therefore, investigation of harmful impacts of different environmental pollutants, including herbicides, is vital to knowing the mechanisms of toxicity and devising control strategies. The current experiment included bighead carp ( n = 80 ) to estimate adverse impacts. Fish were randomly placed in 4 different experimental groups (T0-T3) and were treated for 36 days with acetochlor at 0, 300, 400, and 500 μg/L. Fresh blood without any anticoagulant was obtained and processed for nuclear and morphological changes in erythrocytes. At the same time, various visceral organs, including the gills, liver, brain, and kidneys, were removed and processed on days 12, 24, and 36 to determine oxidative stress and various antioxidant biomarkers. Comet assays revealed significantly increased DNA damage in isolated cells of the liver, kidneys, brain, and gills of treated fish. We recorded increased morphological and nuclear changes ( P ≤ 0.05 ) in the erythrocyte of treated fish. The results on oxidative stress showed a higher quantity of oxidative biomarkers and a significantly ( P ≤ 0.05 ) low concentration of cellular proteins in the gills, liver, brain, and kidneys of treated fish compared to unexposed fish. Our research findings concluded that acetochlor renders oxidative stress in bighead carp.

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“…DNA damage was only observed in hemocytes of the freshwater clam Corbicula fluminea exposed to a herbicide mixture of Roundup ® (2 and 10 mg/L) and atrazine (2 and 10 µg/L) for 96 h, but no genotoxic effects were observed when clams were exposed to the herbicides separately using the same concentrations [ 65 ]. In another study, a mixture of endo-sulfan and CPF (0.94 to 1.88 µg/L) also caused significant DNA damage to erythrocytes of fingerlings of Nile tilapia Oreochromis niloticus , after a 70-days exposure [ 66 ]. The genotoxicity of mixtures of metals has also been studied using the micronucleus assay on erythrocytes of fish, Synodontis clarias and Tilapia nitolica , exposed to Cu and zinc (Zn) [ 67 ].…”

Section: Discussionmentioning

confidence: 99%

Environmentally Relevant Mixture of Pesticides Affect Mobility and DNA Integrity of Early Life Stages of Rainbow Trout (Oncorhynchus mykiss)

Santos

Cachot

Cormier

et al. 2021

Toxics

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The aim of this study was to analyze the impact of three concentrations of a pesticide mixture on the first development stages of rainbow trout (Oncorhynchus mykiss). The mixture was made up of three commonly used pesticides in viticulture: glyphosate (GLY), chlorpyrifos (CPF) and copper sulfate (Cu). Eyed stage embryos were exposed for 3 weeks to three concentrations of the pesticide mixture. Lethal and sub-lethal effects were assessed through a number of phenotypic and molecular endpoints including survival, hatching delay, hatching success, biometry, swimming activity, DNA damage (Comet assay), lipid peroxidation (TBARS), protein carbonyl content and gene expression. Ten target genes involved in antioxidant defenses, DNA repair, mitochondrial metabolism and apoptosis were analyzed using real-time RT-qPCR. No significant increase of mortality, half-hatch, growth defects, TBARS and protein carbonyl contents were observed whatever the pesticide mixture concentration. In contrast, DNA damage and swimming activity were significantly more elevated at the highest pesticide mixture concentration. Gene transcription was up-regulated for genes involved in detoxification (gst and mt1), DNA repair (ogg1), mitochondrial metabolism (cox1 and 12S), and cholinergic system (ache). This study highlighted the induction of adaptive molecular and behavioral responses of rainbow trout larvae when exposed to environmentally realistic concentrations of a mixture of pesticides.

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Pesticide Mixture Induced DNA Damage in Peripheral Blood Erythrocytes of Freshwater Fish, Oreochromis niloticus

Cited by 12 publications

References 12 publications

Comparative assessment of the acute toxicity, haematological and genotoxic effects of ten commonly used pesticides on the African Catfish, Clarias gariepinus Burchell 1822

Comparative assessment of the acute toxicity, haematological and genotoxic effects of ten commonly used pesticides on the African Catfish, Clarias gariepinus Burchell 1822

[Retracted] Acetochlor Affects Bighead Carp (Aristichthys Nobilis) by Producing Oxidative Stress, Lowering Tissue Proteins, and Inducing Genotoxicity

Environmentally Relevant Mixture of Pesticides Affect Mobility and DNA Integrity of Early Life Stages of Rainbow Trout (Oncorhynchus mykiss)

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