Pesquisa de anticorpos contra Leptospira spp. em animais silvestres e em estado feral da região de Nhecolândia, Mato Grosso do Sul, Brasil: utilização da técnica de imuno-histoquímica para detecção do agente

Gírio, Raul José Silva; Pereira, Fernando Lúcio Garcia; Filho, Moacir Marchiori; Mathias, Luís Antônio; Herreira, Rita de Cássia Pereira; Alessi, Antônio Carlos; Girio, T. M. S.

doi:10.1590/s0103-84782004000100025

Cited by 25 publications

(13 citation statements)

References 6 publications

(4 reference statements)

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“…The high frequency of low titers found in this study is common in most studies (Girio et al 2004, Esteves et al 2005, Souza-Júnior et al 2006, Silva et al 2010. Wild carnivores are sentinels for leptospirosis (Millán et al 2009), indicating the occurrence of the disease in the environment.…”

Section: Discussionsupporting

confidence: 57%

Occurrence of antibodies against Leptospira spp. In free-ranging wild canids from the Brazilian savanna

et al. 2015

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The Brazilian savanna, also known as Cerrado, is one of the world's richest and most ecologically invaluable tropical savanna regions. There are few studies in Brazil about the diseases that affect the wild canids of this biome, which may be harmful to wildlife populations and public health. The aim of this study was to evaluate the occurrence of antibodies against Leptospira spp. in three Cerrado wild canids species using the microscopic agglutination test (MAT). Serum samples were tested from 19 crab-eating foxes (Cerdocyon thous), 14 maned wolves (Chrysocyon brachyurus), and seven hoary foxes (Lycalopex vetulus), all free-ranging animals found in the municipalities of Araguari and Uberlândia, Minas Gerais State, and Cumari, Goiás State, Brazil. Fourteen (35%) of these samples were seropositive. The most frequent serovars detected in the samples were Copenhageni and Hardjo, but reactions to the serovars Autumnalis, Grippotyphosa, Hebdomadis, Wolffi, and Icterohaemorrhagiae also occurred. Notwithstanding other reported results, this study is the first to report the presence of antibodies against Leptospira spp. in L. vetulus. The three species of wild canids examined may act as potential hosts for several serovars of leptospira in Brazil's savanna environment.

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Section: Discussionsupporting

confidence: 57%

Occurrence of antibodies against Leptospira spp. In free-ranging wild canids from the Brazilian savanna

et al. 2015

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“…in Pantanal (GIRIO et al, 2004). At BVBS, only one animal (3.1%) was positive for the serogroup Serjoe.…”

Section: Introductionmentioning

confidence: 97%

“…A serological comparison between two distinct pampas deer populations showed that there was no exposure to Leptospira spp. in the population from a dry region (Emas National Park, Goiás, Brazil) while 9.7% (4/41) (GIRIO et al, 2004) and 24% (4/17) (MATHIAS et al, 1999) of the animals from the wetland region (Pantanal, Mato Grosso do Sul, Brazil) were seropositive for the serogroups Serjoe and Mini. In contrast, none of the ten M. americana investigated were positive List of Leptospira spp.…”

Section: Introductionmentioning

confidence: 99%

Occurrence of antibodies anti -Toxoplasma gondii, Neospora caninum and Leptospira interrogans in a captive deer herd in Southern Brazil

Zimpel

Grazziotin

Filho

et al. 2015

Rev. Bras. Parasitol. Vet.

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A large number of Brazilian zoos keep many endangered species of deer, however, very few disease surveillance studies have been conducted among captive cervids. Blood samples from 32 Brazilian deer (Blastocerus dichotomus, Mazama nana and Mazama americana) kept in captivity at Bela Vista Biological Sanctuary (Foz do Iguaçu, Brazil) were investigated for 10 ruminant pathogens, with the aims of monitoring deer health status and evaluating any potential zoonotic risk. Deer serum samples were tested for Brucella abortus, Leptospira (23 serovars), Toxoplasma gondii, Neospora caninum, bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, foot-and-mouth disease virus, western equine encephalitis virus, eastern equine encephalitis virus and Venezuelan equine encephalitis virus. Antibodies against T. gondii (15.6%), N. caninum (6.2%) and L. interrogans serogroup Serjoe (3.1%) were detected. The serological results for all other infectious agents were negative. The deer were considered to be clinically healthy and asymptomatic regarding any disease. Compared with studies on free-ranging deer, the prevalences of the same agents tested among the captive deer kept at the Sanctuary were lower, thus indicating good sanitary conditions and high-quality management practices at the zoo.

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“…foi observada somente em um dos órgãos testados. No entanto, os demais também apresentaram lesões compatíveis com leptospirose, embora nestes não houvesse imunomarcações [13]. A ausência da imunomarcação não determina a ausência do agente no tecido, pois muitas podem ser as razões que impedem tais reações, como por exemplo, a distribuição ao acaso das leptospiras nos tecidos fixados, levando à ausência do agente em alguns campos observados [10,14].…”

Section: Discussionunclassified

“…Para casos em que durante a necropsia não há suspeita da doença, porém, observam-se lesões sugestivas através do exame histopatológico, a imuno-histoquímica (IHQ) é indicada e apresenta uma boa especificidade [13].…”

Section: Introductionunclassified

Role of Immunohistochemistry in the Diagnosis of Leptospirosis in Neotropical Primates

Grumann

Silva

Luz

et al. 2017

Acta Scientiae. Vet.

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Background: Leptospirosis is considered the most widespread zoonosis worldwide, occurring more frequently in tropical and developing regions. The aim of the present study was to detect the presence of Leptospira spp. in different primate tissues, using immunohistochemical (IHC) assays, taking advantage of the considerable number of necropsies compatible with a diagnosis of leptospirosis in neotropical primates at the Animal Pathology Laboratory (APL) of the University of Passo Fundo (UPF) in the northern region of Rio Grande do Sul. Materials, Methods & Results: Paraffin-embedded primate tissue samples were selected from necropsy examinations and subjected to IHC. The streptavidin-biotin-peroxidase method was used with diaminobenzidine chromogen (DAB) to verify immunostaining. Of the101 primates tested for Leptospira spp., 51.48% were positive; taining was distributed between lung (76.92%), liver (44.23%), and kidney (32.69%) tissue. Analysis of the combined anatomopathological verification data of the studied organs revealed a high frequency of lesions commonly observed in the tissues of animals exposed to the pathogen. For complementary diagnosis, an anti-Leptospira spp. antibody test was performed in primates at the UPF-Zoo, from which a population of the necropsied animals originated. The microscopic agglutination test (MAT) was utilized, which demonstrated 90.47% positivity in 21 individuals; sejroe and panama were the most frequent serovars. Discussion: Different intensities of tissue immunostaining were observed. Areas of fragmented or diffuse staining were considered to indicate equal positivity to that indicated by areas of staining with preserved morphology. Of 52 Leptospirapositive primates, most presented some degree of staining in lung samples, which shows a high level of involvement for this organ in primate leptospirosis. Conventional pathological diagnostic methods do not allow fort issue antigen recognition, thus making the IHC technique important to facilitate conclusive antigen sample verification. In the liver, leptospires were detected mostly between the sinusoids, hepatocytes, and Kupffer cells. In kidney tissues, staining indicated small agglomerates in the tubular lumen, interstitium, and glomeruli. All these forms of presentation have been previously reported. Considering that we detected the highest number of positive samples in lung tissue, followed by those from liver and kidney tissue, we argue that the IHC technique, when applied to samples of these three tissues, decreases the chance of false negatives. Anatomopathological studies of primate leptospirosis are scarce. In dogs, renal lesions are characterized by the necrosis and degeneration of tubular epithelium, cellular debris, and hyaline cylinders. In the liver, hepatocyte cord dissociation and biliary pigment accumulation within the canaliculi and hepatocellular necrosis are observed. These findings are similar to those from our study. In the lung, diffuse alveolar lesions are reported, with hemorrhage and edema, in add...

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Pesquisa de anticorpos contra Leptospira spp. em animais silvestres e em estado feral da região de Nhecolândia, Mato Grosso do Sul, Brasil: utilização da técnica de imuno-histoquímica para detecção do agente

Cited by 25 publications

References 6 publications

Occurrence of antibodies against Leptospira spp. In free-ranging wild canids from the Brazilian savanna

Occurrence of antibodies against Leptospira spp. In free-ranging wild canids from the Brazilian savanna

Occurrence of antibodies anti -Toxoplasma gondii, Neospora caninum and Leptospira interrogans in a captive deer herd in Southern Brazil

Role of Immunohistochemistry in the Diagnosis of Leptospirosis in Neotropical Primates

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