Pesquisa de anticorpos anti-Neospora spp. e anti-herpersvírus equino em cavalos de tração no município de Santa Maria, RS, Brasil

Sangioni, Luís Antonio; Botton, Sônia de Ávila; Cargnelutti, Juliana Felipetto; Cadore, Gustavo Cauduro; Cezar, Alfredo Skrebsky; Weiblen, Rudi; Lopes, Sonia T.A.; Vogel, Fernanda Silveira Flôres

doi:10.1590/s0103-84782011000200023

Cited by 7 publications

(5 citation statements)

References 17 publications

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“…is relatively high when compared with another study performed in southern Brazil, in which 15.4% seropositivity was found in draft horses (SANGIONI et al, 2011) and 23.9% detected in horses from state of Minas Gerais (RIBEIRO et al, 2016). Moreover, Hoane et al (2006) detected a very low prevalence (2.5%) of antibodies against Neospora spp.…”

Section: Discussionmentioning

confidence: 54%

Antibodies against Apicomplexa protozoa and absence sarcocysts in heart tissues from horses in southern Brazil

Portella

Cadore²,

Sangioni³

et al. 2017

Rev. Bras. Parasitol. Vet.

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Sarcocystis spp., Neospora spp., and Toxoplasma gondii are Apicomplexa protozoa that can infect horses. This study aimed to investigate the occurrence of antibodies against Sarcocystis spp., Neospora spp., and T. gondii in horses slaughtered in southern Brazil. The presence of histological lesions, tissue cysts, and Sarcocystis spp. DNA in the hearts of these horses was also investigated. A total of 197 paired serum and heart samples were evaluated by serology and direct microscopic examination; 50 of these samples were subjected to histopathological and PCR analyses. Antibodies against at least one of the protozoa were detected in 146 (74.1%) of the serum samples. The frequencies of positive serology were: 36% (71/197) against Sarcocystis spp., 39.1% (77/197) against Neospora spp., and 47.2% (93/197) against T. gondii. No cysts, Sarcocystis spp. DNA, or histopathological lesions were observed in myocardial tissue samples. The frequencies of antibody seropositivity against Sarcocystis spp., Neospora spp., and T. gondii showed that horses are frequently infected by these parasites in southern Brazil. The absence of sarcocysts in horse tissues is compatible with their role as aberrant/accidental hosts in the life cycle of Sarcocystis spp.. Keywords: Accidental hosts, epidemiology, Sarcocystis spp., Neospora spp., Toxoplasma gondii. ResumoSarcocystis spp., Neospora spp. e Toxoplasma gondii são protozoários que pertencem ao filo Apicomplexa e que podem afetar equinos. O objetivo deste estudo foi investigar a ocorrência de anticorpos contra Sarcocystis spp., Neospora spp. e T. gondii. A presença de lesões histológicas, cistos teciduais e DNA de Sarcocystis spp. no miocárdio de equinos abatidos no sul do Brasil também foi investigado. Um total de 197 amostras de soro juntamente com as respectivas amostras de coração, foram avaliadas por sorologia e exame microscópico direto. Destas amostras, 50 foram selecionadas e submetidas a análise histopatológica e PCR. Anticorpos contra pelo menos um dos protozoários foi detectado em 146 (74,1%) das amostras de soro. As frequências de sorologia positiva foram: 36% (71/197) para Sarcocystis spp., 39,1% (77/197) para Neospora spp. e 47,2% (93/197) para T. gondii. Não foram encontradas lesões histopatológicas, cistos e DNA de Sarcocystis spp. nas amostras de miocárdio dos equinos. As frequências de soropositividade para Sarcocystis spp., Neospora spp. e T. gondii mostra que os equinos podem ser frequentemente infectados por estes parasitas no sul do Brasil. A ausência de sarcocistose no coração dos equinos é compatível com seu papel como hospedeiro errático/acidental no ciclo de vida deste protozoário.

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Section: Discussionmentioning

confidence: 54%

Antibodies against Apicomplexa protozoa and absence sarcocysts in heart tissues from horses in southern Brazil

Portella

Cadore²,

Sangioni³

et al. 2017

Rev. Bras. Parasitol. Vet.

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“…In a study on 91 cart horses (no defined breed) and 123 Crioula breed horses in Rio Grande do Sul, Toscan et al (2011) found that there was high seropositivity in both groups (15.4% and 16.3%, respectively), and no difference between the distinct populations was observed. However, in Santa Maria, Rio Grande do Sul, in traction horses (no pure breed), Sangioni et al (2011) also found high seropositivity (15.4%) by means of IFAT (1:50).…”

Section: Resultsmentioning

confidence: 80%

Neospora spp. antibodies in horses from two geographical regions of the state of Santa Catarina, Brazil

Moura

Silva

Farias

et al. 2013

Rev. Bras. Parasitol. Vet.

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The aims of this study were to determine occurrences of Neospora spp. IgG antibodies in horses from two geographical regions of the state of Santa Catarina, southern Brazil, and identify risk factors for infection. Analyses were performed on 615 samples using the immunofluorescent antibody test (IFAT ≥ 1:50). Out of the 615 samples, 25 (4.1%) were positive for Neospora spp. The titers for Neospora spp. were distributed as follows: 1:50 (13), 1:100 (eight), 1:200 (three) and 1:400 (one). Out of the 311 samples taken in the mountain region, eight were positive (2.6%). Among the samples from the coastal region (304), 17 had Neospora spp. antibodies, thus indicating occurrence of 5.6%. Although no statistically significant difference was observed (P = 0.06704), the prevalence among animals of the coast was 2.2 times higher than that of the mountain region. Contact with dogs and/or cattle (P = 0.007596) were identified as risk factor for Neospora spp. infection.

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“…Sabe-se que a ampla distribuição do HVE no Brasil tem sido descrita na literatura científica, com ocorrências mais baixas que a observada em nosso estudo (32%) de [26] no Paraná e 0% (0/91) [32] e 4,5% (67/1506) [10] no Rio Grande do Sul.…”

Section: Resultsunclassified

“…O Herpesvirus Equino (HVE) e o Vírus da Arterite Viral Equina (VAVE), possuem elevada capacidade de disseminação, permanecendo latentes no organismo, até que o animal apresente imunossupressão [12,19], levando a surtos de abortos, morte neonatal, quadros neurológicos e respiratórios [13,17,27,34,37]. Os levantamentos sorológicos de HVE, no Nordeste, foram realizados em Mossoró, Rio Grande do Norte, com equinos de vaquejada [14] e região metropolitana de Fortaleza, Ceará [2] com ocorrências de 19,3% (22/114) e 41,2% (28/68) de positivos respectivamente, enquanto outros estudos nacionais variaram de 0 a 87% [1,2,[7][8][9][10]14,15,20,[23][24][25]27,30,32,38]. O vírus já foi isolado no Brasil, em casos de doença neurológica [6], abortamentos e mortalidade perinatal [34].…”

Section: Introductionunclassified

Occurrence of Antibodies against Herpesvirus and Arteritis Virus in Equines from the State of Rio Grande do Norte, Brazil

Rocha

Diniz

Jesus

et al. 2020

Acta Scientiae. Vet.

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Background: Equine Herpesvirus type 1 (EHV-1) and Equine Arteritis Virus (EAV) have a high capacity for dissemination, causing losses in horse breeding due to reproductive, respiratory and neurological problems. In the State of Rio Grande do Norte there is only one survey, in the municipality of Mossoró, about these diseases. The purpose of this study was therefore to determine the prevalence and assess the risk factors associated with EHV-1 and EAV infections in unvaccinated horses raised in the mesoregions of East and West Potiguar in the State of Rio Grande do Norte, Brazil.Materials, Methods & Results: Between August 2018 and February 2019, blood samples were collected from equines on farms located in sixteen municipalities in the mesoregions of East and West Potiguar for the serological diagnosis of EAV (809 samples from 90 farms) and of EHV (778 samples from 88 farms), being that, only municipalities that had at least five hundred equines were included in the study. An investigative epidemiological questionnaire was applied to each property visited, containing questions related to the breeder, property, animal and the sanitary, reproductive and nutritional management. A logistic regression was performed to assess the risk factors associated with EVH, considering the serological test (positive or negative) as the dependent variable. EAV and EVH antibodies were detected through the serum virus neutralization assay. No evidence was found of EAV [0% (0/809)], but EHV showed a prevalence rate of 32% (249/778), with 80.6% (71/88) of the farms having at least one animal that tested positive, and EHV was present in all the municipalities covered in this study. The risk factors identified were: animals raised in East Potiguar (OR = 1.36, CI 95%: 1.01-1.85, P = 0.041), extensive livestock farming system (OR = 1.79, CI 95%: 1.10-2.91, P = 0.041), working animals (OR = 3.63, IC 95%: 1.91-6.91, P = 0.000), unclean facilities (OR = 2.32, CI 95%: 1.27-4.33, P = 0.006), and non-disinfected facilities (OR = 1.83, CI 95%: 1.15-2.91, P = 0.009).Discussion: The absence of horses seropositive for EAV confirms the findings reported for 132 cattle herding horses in the municipality of Mossoró, State of Rio Grande do Norte, Brazil, and by other national surveys that report zero or low occurrence rates. EAV is therefore considered an exotic disease that has not yet been diagnosed nor its etiological agent isolated in this country. EHV was found to be distributed in the two mesoregions, indicating a higher occurrence among working horses raised in the extensive livestock farming system on these farms, which were more physically fatigued and liable to be immunosuppressed, making them prone to infection, especially when left to graze in contact with other animals, although sports horses have also been found to be at high risk of becoming infected. The risk factor analysis also indicated the importance of hygiene and disinfection of livestock premises, where other biosafety measures such as quarantine and vaccination are likely not taken, and animals circulate unrestrictedly, facilitating the dissemination of the virus. Studies are therefore needed to shed light on the real situation of the presence of EAV throughout Brazil’s territory. On the other hand, EHV is widely disseminated throughout the state, and the risk factors for this disease not only underscore the importance of proper sanitary management for control of the disease but also serve as a warning to authorities and animal health professionals.

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Pesquisa de anticorpos anti-Neospora spp. e anti-herpersvírus equino em cavalos de tração no município de Santa Maria, RS, Brasil

Cited by 7 publications

References 17 publications

Antibodies against Apicomplexa protozoa and absence sarcocysts in heart tissues from horses in southern Brazil

Antibodies against Apicomplexa protozoa and absence sarcocysts in heart tissues from horses in southern Brazil

Neospora spp. antibodies in horses from two geographical regions of the state of Santa Catarina, Brazil

Occurrence of Antibodies against Herpesvirus and Arteritis Virus in Equines from the State of Rio Grande do Norte, Brazil

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