Peroxynitrite participates in mechanisms involved in capacitation of cryopreserved cattle

Rodriguez, P.; Beconi, M.T.

doi:10.1016/j.anireprosci.2007.12.017

Cited by 34 publications

(28 citation statements)

References 52 publications

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“…Then, the DHR oxidation was confirmed in the spermatozoa exposed to SIN-1 compared with the untreated sperm, demonstrating that with SIN-1 the effects of peroxynitrite could be analyzed on different cell variables. We found that sperm viability was unchanged after treatment with concentrations up to 1.0 mmol/l of SIN-1 during an incubation time up to 4 h. These data are consistent with a previous study conducted on cryopreserved bovine spermatozoa, where incubation for 45 min at 378C with up to 1.0 mmol/l of SIN-1 did not significantly reduce sperm viability (Rodriguez and Beconi, 2009). Considering that these SIN-1 concentrations did not alter sperm viability, we proceeded to evaluate motility.…”

Section: Discussionsupporting

confidence: 82%

“…In a study using peroxynitrite, a decrease in human sperm motility was observed, which was associated with increased levels of lipid peroxidation (Oztezcan et al, 1999). Later, in bovine spermatozoa, a significant decrease in the motility of cryopreserved bull spermatozoa was reported after incubation with 160 mmol/l SIN-1 (Rodriguez and Beconi, 2009). Although we also worked with SIN-1, higher SIN-1 concentrations were needed to significantly decrease sperm motility.…”

Section: Discussionmentioning

confidence: 99%

“…SIN-1 has been used in studies with several cell types, including human and bovine spermatozoa (Weinberg et al, 1995;Blanco Garcia et al, 2009;Rodriguez and Beconi, 2009;Agbani et al, 2011). In this study, the suitable generation of peroxynitrite with SIN-1 was monitored using DHR, which is selectively oxidized by peroxynitrite (Blanco Garcia et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

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Peroxynitrite-mediated nitrosative stress decreases motility and mitochondrial membrane potential in human spermatozoa

Uribe

Boguen

Treulén

et al. 2014

MHR: Basic Science of Reproductive Medicine

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Nitrosative stress is produced by high levels of reactive nitrogen species (RNS). The RNS include peroxynitrite, a highly reactive free radical produced from a diffusion-controlled reaction between nitric oxide and superoxide anion. Peroxynitrite causes nitration and oxidation of lipids, proteins and DNA, and is thus considered an important pathogenic mechanism in various diseases. Although high levels of peroxynitrite are associated with astenozoospermia, few reports exist regarding the in vitro effect of high levels of this RNS on human sperm. The aim of this study was to evaluate the in vitro effect of nitrosative stress caused by peroxynitrite on the viability, motility and mitochondrial membrane potential of human spermatozoa. To do this, human spermatozoa from healthy donors were exposed in vitro to 3-morpholinosydnonimine (SIN-1), a molecule that generates peroxynitrite. Incubations were done at 378C for up to 4 h with SIN-1 concentrations between 0.2 and 1.0 mmol/l. Generation of peroxynitrite was confirmed using dihydrorhodamine 123 (DHR) by spectrophotometry and flow cytometry. Sperm viability was assessed by propidium iodide staining; sperm motility was analyzed by CASA, and the state of mitochondrial membrane potential (DCm) by JC-1 staining. Viability and DCm were measured by flow cytometry. The results showed an increase in DHR oxidation, demonstrating the generation of peroxynitrite through SIN-1. Peroxynitrite decreased progressive and total motility, as well as some sperm kinetic parameters. Mitochondrial membrane potential also decreased. These alterations occurred with no decrease in sperm viability. In conclusion, peroxynitriteinduced nitrosative stress impairs vital functions in the male gamete, possibly contributing to male infertility.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Peroxynitrite-mediated nitrosative stress decreases motility and mitochondrial membrane potential in human spermatozoa

Uribe

Boguen

Treulén

et al. 2014

MHR: Basic Science of Reproductive Medicine

View full text Add to dashboard Cite

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“…Strategies to stimulate capacitation, through the depletion of cholesterol from the sperm membrane, or the triggering of capacitation-specific sperm signalling pathways, are emerging and have been shown to increase the success of IVF in rodents (Takeo et al 2008, Seita et al 2009). However, the hypothesis that oxidants (especially peroxynitrite) could prepare spermatozoa for fertilisation has not been tested, despite their ability to initiate capacitation (Rodriguez & Beconi 2009, Rodriguez et al 2011. Such studies may shed light on achieving an optimal balance between antioxidant inclusion, to prevent sperm damage during handling and pro-oxidant concentration to trigger capacitation when required.…”

Section: General Conclusionmentioning

confidence: 99%

“…IVF treatments that are currently employed (5) include bicarbonate (stimulates adenylate cyclase/cAMP/protein kinase A and tyrosine kinase signalling pathways) heparin (enhances stripping of the sperm surface) and fatty acid-free albumin (depletes cholesterol). Novel stimulation methods include cholesterol-accepting vesicles (in white) or cholesterol-free cyclodextrin (in red) or the use of certain pro-oxidants (6) (Rodriguez & Beconi 2009, Rodriguez et al 2011. For further details and references, see section 'Novel sperm surface manipulation methods'.…”

Section: Stabilisation Of the Sperm Surface With Seminal Plasma Proteinsmentioning

confidence: 99%

Sperm surface changes and physiological consequences induced by sperm handling and storage

2011

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Spermatozoa interact with their immediate environment and this contact remodels the sperm surface in preparation for fertilisation. These fundamental membrane changes will be critically covered in this review with special emphasis on the very specific surface destabilisation event, capacitation. This process involves very subtle and intricate modifications of the sperm membrane including removal of suppression (decapacitation) factors and changes in the lateral organisation of the proteins and lipids of the sperm surface. Processing of sperm for assisted reproduction (storage, sex-sorting, etc.) subjects spermatozoa to numerous stressors, and it is possible that this processing overrides such delicate processes resulting in sperm instability and cell damage. To improve sperm quality, novel mechanisms must be used to stabilise the sperm surface during handling. In this review, different types of membrane stress are considered, as well as novel surface manipulation methods to improve sperm stability.

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Reactive oxygen, nitrogen, and sulfur species in human male fertility. A crossroad of cellular signaling and pathology

et al. 2019

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Infertility is a significant global health problem that currently affects one of six couples in reproductive age. The quality of male reproductive cells dramatically decreased over the last years and almost every aspect of modern life additionally worsen sperm functional parameters that consequently markedly increase male infertility. This clearly points out the importance of finding a new approach to treat male infertility. Redox signaling mediated by reactive oxygen, nitrogen and sulfur species (ROS, RNS, and RSS respectively), has appeared important for sperm reproductive function. Present review summarizes the current knowledge of ROS, RNS, and RSS in male reproductive biology and identifies potential targets for development of novel pharmacological and therapeutic approaches for male infertility by targeted therapeutic modulation of redox signaling.

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Peroxynitrite participates in mechanisms involved in capacitation of cryopreserved cattle

Cited by 34 publications

References 52 publications

Peroxynitrite-mediated nitrosative stress decreases motility and mitochondrial membrane potential in human spermatozoa

Peroxynitrite-mediated nitrosative stress decreases motility and mitochondrial membrane potential in human spermatozoa

Sperm surface changes and physiological consequences induced by sperm handling and storage

Reactive oxygen, nitrogen, and sulfur species in human male fertility. A crossroad of cellular signaling and pathology

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