Peroxisome proliferator-activated receptor gamma (PPARγ) in brown trout: Interference of estrogenic and androgenic inputs in primary hepatocytes

Lopes, Célia; Madureira, Tânia Vieira; Ferreira, Nádia; Pinheiro, Ivone; Castro, L. Filipe C.; Rocha, Eduardo

doi:10.1016/j.etap.2016.08.009

Cited by 10 publications

(6 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These compounds affect the PPAR pathway either by directly binding the LBD or through direct transcriptional effects on PPARγ target genes, commonly resulting in metabolic disorders. Although previous studies have indicated that some exogenous steroids, including trenbolone acetate, inhibit PPARγ expression (Salehzadeh et al 2011; Chung et al 2012; Lopes et al 2016), these studies examined PPARγ expression in skeletal muscle and primary hepatocytes in mammals and fish. In contrast, in the present study, we observed an up‐regulation of PPARγ.…”

Section: Discussionmentioning

confidence: 99%

Sex‐ and Developmental Stage–Related Differences in the Hepatic Transcriptome of Japanese Quail (Coturnix japonica) Exposed to 17β‐Trenbolone

Mittal

Henry

Cornman

et al. 2021

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Endocrine‐disrupting chemicals can cause transcriptomic changes that may disrupt biological processes associated with reproductive function including metabolism, transport, and cell growth. We investigated effects from in ovo and dietary exposure to 17β‐trenbolone (at 0, 1, and 10 ppm) on the Japanese quail (Coturnix japonica) hepatic transcriptome. Our objectives were to identify differentially expressed hepatic genes, assess perturbations of biological pathways, and examine sex‐ and developmental stage–related differences. The number of significantly differentially expressed genes was higher in embryos than in adults. Male embryos exhibited greater differential gene expression than female embryos, whereas in adults, males and females exhibited similar numbers of differentially expressed genes (>2‐fold). Vitellogenin and apovitellenin‐1 were up‐regulated in male adults exposed to 10 ppm 17β‐trenbolone, and these birds also exhibited indications of immunomodulation. Functional grouping of differentially expressed genes identified processes including metabolism and transport of biomolecules, enzyme activity, and extracellular matrix interactions. Pathway enrichment analyses identified as perturbed peroxisome proliferator–activated receptor pathway, cardiac muscle contraction, gluconeogenesis, growth factor signaling, focal adhesion, and bile acid biosynthesis. One of the primary uses of 17β‐trenbolone is that of a growth promoter, and these results identify effects on mechanistic pathways related to steroidogenesis, cell proliferation, differentiation, growth, and metabolism of lipids and proteins. Environ Toxicol Chem 2021;40:2559–2570. © 2021 SETAC. This article has been contributed to by US Government employees and their work is in the public domain in the USA.

show abstract

Section: Discussionmentioning

confidence: 99%

Sex‐ and Developmental Stage–Related Differences in the Hepatic Transcriptome of Japanese Quail (Coturnix japonica) Exposed to 17β‐Trenbolone

Mittal

Henry

Cornman

et al. 2021

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

show abstract

“…In male zebrafish ( Danio rerio ), 4 ng/L EE2 exposure for 90 days post-fertilization was related to the build-up of adipocytes in the testis, suggesting signs of early obesity [ 40 ]. An in vitro study on the livers of brown trout indicated that EE2, as with other estrogenic compounds, interfered with the activity of PPAR-γ, with a significant (3-fold) decrease in response to 50 µM EE2 [ 15 ]. In adult male ten spotted live-bearer fish ( Cnesterodon decemmaculatus) , exposure to a concentration of EE2 up to 200 ng/L was associated with increased steatosis, necrosis, and disruption of acinar organization (effects detected starting from 100 ng/L EE2) [ 41 ].…”

Section: Environmental and Human Health Implications Of Edc Exposurementioning

confidence: 99%

“…EDCs interfere with many hormone-regulated physiological pathways and have complex effects on human and fish physiology due to the diversity of the hormone receptors and enzymes that they bind [ 10 ]. The detailed mechanisms by which ED compounds act is not completely understood, but many interact with nuclear receptors, such as the retinoid X receptor (RXR) [ 11 , 12 , 13 , 14 ], peroxisome proliferator-activated receptors (PPARs) [ 14 , 15 , 16 , 17 , 18 ] and estrogen receptors (ERs) [ 19 , 20 ]. The binding of EDCs to these and other receptors, as well as steroidogenic enzymes, can perturb normal physiological processes.…”

Section: Introductionmentioning

confidence: 99%

Integrated Genomic and Bioinformatics Approaches to Identify Molecular Links between Endocrine Disruptors and Adverse Outcomes

Verga

Huff

Owens

et al. 2022

IJERPH

View full text Add to dashboard Cite

Exposure to Endocrine Disrupting Chemicals (EDC) has been linked with several adverse outcomes. In this review, we examine EDCs that are pervasive in the environment and are of concern in the context of human, animal, and environmental health. We explore the consequences of EDC exposure on aquatic life, terrestrial animals, and humans. We focus on the exploitation of genomics technologies and in particular whole transcriptome sequencing. Genome-wide analyses using RNAseq provides snap shots of cellular, tissue and whole organism transcriptomes under normal physiological and EDC perturbed conditions. A global view of gene expression provides highly valuable information as it uncovers gene families or more specifically, pathways that are affected by EDC exposures, but also reveals those that are unaffected. Hypotheses about genes with unknown functions can also be formed by comparison of their expression levels with genes of known function. Risk assessment strategies leveraging genomic technologies and the development of toxicology databases are explored. Finally, we review how the Adverse Outcome Pathway (AOP) has exploited this high throughput data to provide a framework for toxicology studies.

show abstract

“…Androgens and estrogens are the primary types of sex steroids. Exogenous testosterone significantly inhibited the expression of PPAR γ in primary hepatocytes isolated from brown trout [ 11 ]. 17 β -Estradiol could regulate the expression of PPAR γ in human peripheral blood eosinophils [ 12 ].…”

Section: Interaction Between Ppars and Sex Steroidsmentioning

confidence: 99%

Mediating Roles of PPARs in the Effects of Environmental Chemicals on Sex Steroids

Huang

Chen

2017

PPAR Research

View full text Add to dashboard Cite

Peroxisome proliferator-activated receptors (PPARs) are ligand-activated nuclear receptors that are widely involved in various physiological functions. They are widely expressed through the reproductive system. Their roles in the metabolism and function of sex steroids and thus the etiology of reproductive disorders receive great concern. Various kinds of exogenous chemicals, especially environmental pollutants, exert their adverse impact on the reproductive system through disturbing the PPAR signaling pathway. Chemicals could bind to PPARs and modulate the transcription of downstream genes containing PPRE (peroxisome proliferator response element). This will lead to altered expression of genes related to metabolism of sex steroids and thus the abnormal physiological function of sex steroids. In this review, various kinds of environmental ligands are summarized and discussed. Their interactions with three types of PPARs are classified by various data from transcript profiles, PPRE reporter in cell line, in silico docking, and gene silencing. The review will contribute to the understanding of the roles of PPARs in the reproductive toxicology of environmental chemicals.

show abstract

Peroxisome proliferator-activated receptor gamma (PPARγ) in brown trout: Interference of estrogenic and androgenic inputs in primary hepatocytes

Cited by 10 publications

References 70 publications

Sex‐ and Developmental Stage–Related Differences in the Hepatic Transcriptome of Japanese Quail (Coturnix japonica) Exposed to 17β‐Trenbolone

Sex‐ and Developmental Stage–Related Differences in the Hepatic Transcriptome of Japanese Quail (Coturnix japonica) Exposed to 17β‐Trenbolone

Integrated Genomic and Bioinformatics Approaches to Identify Molecular Links between Endocrine Disruptors and Adverse Outcomes

Mediating Roles of PPARs in the Effects of Environmental Chemicals on Sex Steroids

Contact Info

Product

Resources

About