Peroxisomal catalase deficiency modulates yeast lifespan depending on growth conditions

Lefevre, Sophie D.; Veenhuis, Marten; Klei, Ida J. van der

doi:10.18632/aging.100519

Cited by 21 publications

(14 citation statements)

References 57 publications

(59 reference statements)

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“…Since the β -oxidation pathway directly produces H 2 O 2 , the detoxifying catalase activity is of central importance for the redox balance of the organelle [ 16 , 17 ]. It has been shown in yeast that peroxisomal catalase is required also for the tolerance to externally added H 2 O 2 , which suggests that peroxisomes are generally important for cellular ROS detoxification [ 18 ]. An additional peroxisomal antioxidant activity has been described in yeast as the glutathione peroxidase Gpx1, whose activity is generally required for a proper peroxisomal biogenesis [ 19 ].…”

Section: Pro- and Antioxidant Functions Of Peroxisomesmentioning

confidence: 99%

“…Moreover, catalase mutants show phenotypes of accelerated aging in the Caenorhabditis model [ 27 ]. In yeast, loss of catalase function modulates the lifespan depending on the metabolic activity of peroxisomes [ 18 ]. Thus, peroxisomal (together with mitochondrial) activity in maintaining the cellular redox state is generally important for cell survival and health [ 28 , 29 ].…”

Section: Pro- and Antioxidant Functions Of Peroxisomesmentioning

confidence: 99%

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Pro- and Antioxidant Functions of the Peroxisome-Mitochondria Connection and Its Impact on Aging and Disease

Pascual-Ahuir

Manzanares-Estreder

Proft

2017

Oxidative Medicine and Cellular Longevity

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Peroxisomes and mitochondria are the main intracellular sources for reactive oxygen species. At the same time, both organelles are critical for the maintenance of a healthy redox balance in the cell. Consequently, failure in the function of both organelles is causally linked to oxidative stress and accelerated aging. However, it has become clear that peroxisomes and mitochondria are much more intimately connected both physiologically and structurally. Both organelles share common fission components to dynamically respond to environmental cues, and the autophagic turnover of both peroxisomes and mitochondria is decisive for cellular homeostasis. Moreover, peroxisomes can physically associate with mitochondria via specific protein complexes. Therefore, the structural and functional connection of both organelles is a critical and dynamic feature in the regulation of oxidative metabolism, whose dynamic nature will be revealed in the future. In this review, we will focus on fundamental aspects of the peroxisome-mitochondria interplay derived from simple models such as yeast and move onto discussing the impact of an impaired peroxisomal and mitochondrial homeostasis on ROS production, aging, and disease in humans.

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Section: Pro- and Antioxidant Functions Of Peroxisomesmentioning

confidence: 99%

Section: Pro- and Antioxidant Functions Of Peroxisomesmentioning

confidence: 99%

Pro- and Antioxidant Functions of the Peroxisome-Mitochondria Connection and Its Impact on Aging and Disease

Pascual-Ahuir

Manzanares-Estreder

Proft

2017

Oxidative Medicine and Cellular Longevity

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“…Among them are the following "downstream" processes (Figure 2): (1) the maintenance of trehalose homeostasis, a longevity-defining process known to modulate proteostasis in chronologically "young" and "old" cells 2361 (Figure 1A); (2) the maintenance of the homeostasis of glycogen, a reserve carbohydrate whose elevated level in chronologically "young" and "old" cells is a hallmark of carbohydrate metabolism remodeling in yeast cultured under CR 23; (3) the maintenance of the homeostasis of neutral lipids deposited within LD, a process known to play an essential role in regulating longevity of chronologically aging yeast 142392 (Figure 1B); (4) peroxisomal oxidation of fatty acids, a process implicated in yeast chronological aging 14239192 (Figure 1B); (5) the maintenance of the homeostasis of non-esterified ("free") fatty acids and diacylglycerol, whose reduced levels in chronologically "young" and "old" cells are characteristic of lipid metabolism remodeling in yeast cultured under CR - a pattern likely linked to the demonstrated abilities of both lipid species to elicit an age-related form of liponecrotic PCD 1423103 (Figure 1B); (6) the maintenance of a balance between the relative rates of glycolysis and gluconeogenesis, a process known to impact the level of ethanol in chronologically "young" and "old" yeast - thus defining the extent to which this product of glucose fermentation suppresses the longevity-extending process of peroxisomal oxidation of fatty acids 1423 (Figure 1B); (7) the longevity-extending process of mitochondrial translation 23505156; (8) the maintenance of a balance between the relative rates of mitochondrial fusion and fission, a process known to define the size and number of mitochondria, the length of mitochondrial cristae extending from the IMM, and the level of ATP synthesized in mitochondria of chronologically "young" and "old" yeast 14236470; (9) the development of an age-related pattern of susceptibility to chronic oxidative, thermal and osmotic stresses 314233350565962; (10) an age-related form of apoptotic PCD, which in chronologically "young" yeast is manifested in such early hallmark events of this PCD as the fragmentation of a tubular mitochondrial network into individual mitochondria, release of cytochrome c from mitochondria into the cytosol and phosphatidylserine (PS) translocation from the inner to the outer leaflet of the plasma membrane 1423 (Figure 1B); and (11) the development of a pattern of cell susceptibility to an age-related forms of apoptotic and liponecrotic PCD elicited by an exposure to exogenous hydrogen peroxide or palmitoleic acid, respectively 142359103.…”

Section: Cell-autonomous Mechanisms Orchestrate Longevity-defining Cementioning

confidence: 99%

Cell-autonomous mechanisms of chronological aging in the yeast Saccharomyces cerevisiae

Arlia-Ciommo

Leonov

Piano

et al. 2014

MIC

171

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A body of evidence supports the view that the signaling pathways governing cellular aging - as well as mechanisms of their modulation by longevity-extending genetic, dietary and pharmacological interventions - are conserved across species. The scope of this review is to critically analyze recent advances in our understanding of cell-autonomous mechanisms of chronological aging in the budding yeast Saccharomyces cerevisiae. Based on our analysis, we propose a concept of a biomolecular network underlying the chronology of cellular aging in yeast. The concept posits that such network progresses through a series of lifespan checkpoints. At each of these checkpoints, the intracellular concentrations of some key intermediates and products of certain metabolic pathways - as well as the rates of coordinated flow of such metabolites within an intricate network of intercompartmental communications - are monitored by some checkpoint-specific ʺmaster regulatorʺ proteins. The concept envisions that a synergistic action of these master regulator proteins at certain early-life and late-life checkpoints modulates the rates and efficiencies of progression of such processes as cell metabolism, growth, proliferation, stress resistance, macromolecular homeostasis, survival and death. The concept predicts that, by modulating these vital cellular processes throughout lifespan (i.e., prior to an arrest of cell growth and division, and following such arrest), the checkpoint-specific master regulator proteins orchestrate the development and maintenance of a pro- or anti-aging cellular pattern and, thus, define longevity of chronologically aging yeast.

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“…The initial data on the role of peroxisomal catalase suggested that the absence of catalase decreases the lifespan of cells (Petriv & Rachubinski, 2004 ; Koepke et al ., 2007 , 2008 ). Recent studies however indicate that the absence of catalase also can extend the lifespan of cells at conditions that peroxisomal ROS activate anti-aging enzymes (Mesquita et al ., 2010 ; Titorenko & Terlecky, 2011 ; Kawałek et al ., 2013 ).…”

Section: Introductionmentioning

confidence: 99%

The significance of peroxisome function in chronological aging ofSaccharomyces cerevisiae

et al. 2013

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We studied the chronological lifespan of glucose-grown Saccharomyces cerevisiae in relation to the function of intact peroxisomes. We analyzed four different peroxisome-deficient (pex) phenotypes. These included Δpex3 cells that lack peroxisomal membranes and in which all peroxisomal proteins are mislocalized together with Δpex6 in which all matrix proteins are mislocalized to the cytosol, whereas membrane proteins are still correctly sorted to peroxisomal ghosts. In addition, we analyzed two mutants in which the peroxisomal location of the β-oxidation machinery is in part disturbed. We analyzed Δpex7 cells that contain virtually normal peroxisomes, except that all matrix proteins that contain a peroxisomal targeting signal type 2 (PTS2, also including thiolase), are mislocalized to the cytosol. In Δpex5 cells, peroxisomes only contain matrix proteins with a PTS2 in conjunction with all proteins containing a peroxisomal targeting signal type 1 (PTS1, including all β-oxidation enzymes except thiolase) are mislocalized to the cytosol. We show that intact peroxisomes are an important factor in yeast chronological aging because all pex mutants showed a reduced chronological lifespan. The strongest reduction was observed in Δpex5 cells. Our data indicate that this is related to the complete inactivation of the peroxisomal β-oxidation pathway in these cells due to the mislocalization of thiolase. Our studies suggest that during chronological aging, peroxisomal β-oxidation contributes to energy generation by the oxidation of fatty acids that are released by degradation of storage materials and recycled cellular components during carbon starvation conditions.

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Peroxisomal catalase deficiency modulates yeast lifespan depending on growth conditions

Cited by 21 publications

References 57 publications

Pro- and Antioxidant Functions of the Peroxisome-Mitochondria Connection and Its Impact on Aging and Disease

Pro- and Antioxidant Functions of the Peroxisome-Mitochondria Connection and Its Impact on Aging and Disease

Cell-autonomous mechanisms of chronological aging in the yeast Saccharomyces cerevisiae

The significance of peroxisome function in chronological aging ofSaccharomyces cerevisiae

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