Peroxidase Isoforms of Corn Kernels and Corn on the Cob: Preparation and Characteristics

Measurement of Protein Content in Fruit Juices, Wine and Plant Extracts in the Presence of Endogenous Organic Compounds

“…Membrane-bound proteins, solubilized by high salt and washed free of most oils, phenolics and other soluble components of corn, showed only a 1.2-to 1.3-fold increase in protein content by the alkali assay over that measured by the Coomassie assay, averaging 595 vs. 455 µg/g corn kernel, respectively. Similar results were found with soluble protein, protein in oil-carotenoid fractions, and membrane-bound protein which were prepared from corn germ and cob (19). Interference in the Coomassie assay in corn samples may result from lipid interaction with proteins, and may be abetted by endogenous enzymes such as peroxidase and lipoxygenase.…”

“…The alkali assay allowed measurement of protein to a level of 2 µg/mL. The alkali assay is now being used routinely in our laboratory for the measurement of soluble protein from plant tissues, enzyme preparations, wine and fruit juice (18,19,21,41,42).…”

“…A study was made of soluble and membrane-bound peroxidase (19). Corn kernels were homogenized by Ultra-Turrax in piperazine buffer (pH 6.5), and centrifuged.…”

Effects of Blanching on Some Physical Properties and Processing Recovery of Sweet Corn Cobs

“…This process significantly affects the quality of the material (Arroqui et al 2003). However, improper realization of this operation may cause the change of texture, color, the content of nutrients, as well as affect the costs of the whole process (Mallett 1994;Boyes et al 1997).…”

A Putative Peroxidase cDNA from Turnip and Analysis of the Encoded Protein Sequence

“…Other applications are in the pulping and biobleaching of paper [7]. Due to its relatively high thermal stability and wide distribution, peroxidase has been extensively used as an indicator enzyme for the adequacy of blanching treatment for many plant foods [8]. Horseradish roots (Armoracia rusticana) is the major source of heme peroxidases [9].…”