Peroxidase activity and isoenzymes in the culture medium of NaCl adapted tomato suspension cells

Abstract

The medium of tomato (Lycopersicon esculentum) cells adapted to grow in the presence of 15 g 1-1 NaC1 had a higher peroxidase activity than the medium of an unadapted tomato cell line. When the adapted cells were cultured in a medium without NaCI, the value found for peroxidase activity was intermediate. The increase in peroxidase activity was parallel to an increase of lignin-like compounds in the cell walls, as well as to an increased content or appearance of neutral and basic peroxidase isoenzymes. Apparently, the high values of peroxidase activity in the medium of the salt-adapted cells reflect the changed mechanical properties of the cell wall which, in turn, could be related to the salt adaptation process.

The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress

“…In plants, POD protects cells against harmful concentrations of hydroperoxides and helps in a variety of cellular functions (Beauchamp and Fridovich 1971). Increased total peroxidase activities in response to salinity were reported by Sancho et al (1996). A similar increase in POD was also observed after the application of Ni to the leaves of Silene italica (Gabbrielli et al 1987).…”

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“…Anaerobic tissue has a very high redox potential and the soil environment surrounding the roots contains highly reduced forms of metal ions such as iron, which could readily reduce atmospheric oxygen to superoxide. 19 The levels of ascorbate peroxidase and glutathione reductase activity in cv. Pei at various NaCl concentrations are shown in Fig 2 At 150 mM NaCl, the activity of the two enzymes was increased.…”

“…It was reported that in the stress conditions of intense light, high temperature, flood and salinity, free radicals and reactive oxygen molecules are formed. 1,4,6,11,19 Therefore, a scavenging system should be active. Our results showed that cv.…”

“…The increased total peroxidase activities in response to salinity have been reported. [19][20][21] Sancho et al 19 indicated that the increased total peroxidase activity in the medium of salt adapted cellls reflected the changing mechanical properties of the cell wall, which, in turn, could be related to the salt adaptation process, since cell wall properties are known to be modified by salt stress. Earlier reports [20][21] showed a relationship between total peroxidase activity and changes in cell wall and membrane integrity under salt stress.…”

Thermo-Protective Role of 28-Homobrassinolide in <i>Brassica juncea</i> Plants

“…In plants, POD protects cells against harmful concentrations of hydroperoxides and helps in a variety of cellular functions [37]. Increased total peroxidase activities in response to salinity were reported by [38]. A similar increase in POD was also observed after the application of Ni to the leaves of Silene italic [39].…”