Permeability and diffusivity of nitric oxide in human plasma and red cells

Borland, Colin; Moggridge, Geoff D.; Patel, Ruhi; Patel, Suhani; Zhu, Quan; Vuylsteke, Alain

doi:10.1016/j.niox.2018.05.006

Cited by 6 publications

(2 citation statements)

References 23 publications

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“…Arterial flow was assumed to be steady and laminar, with blood modeled as an incompressible Newtonian fluid with density (ρ) of 1,060 kg/m 3 and viscosity (µ) of 4.5 mPa·s. The diffusion coefficient of nitrite in whole blood was assumed to be 3.30 × 10 −9 m 2 /sec ( 40 , 41 ). We assumed all NO entering the bloodstream is immediately converted to nitrite with a resting and exercised rate of NO release taken as 0.00015 and 0.0068 nmol/cm 2 /sec, respectively ( 2 , 42 ).…”

Section: Methodsmentioning

confidence: 99%

Transport of nitrite from large arteries modulates regional blood flow during stress and exercise

Babbs

Goergen

Rayz

2023

Front. Cardiovasc. Med.

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BackgroundAcute cardiovascular stress increases systemic wall shear stress (WSS)–a frictional force exerted by the flow of blood on vessel walls–which raises plasma nitrite concentration due to enhanced endothelial nitric oxide synthase (eNOS) activity. Upstream eNOS inhibition modulates distal perfusion, and autonomic stress increases both the consumption and vasodilatory effects of endogenous nitrite. Plasma nitrite maintains vascular homeostasis during exercise and disruption of nitrite bioavailability can lead to intermittent claudication.HypothesisDuring acute cardiovascular stress or strenuous exercise, we hypothesize enhanced production of nitric oxide (NO) by vascular endothelial cells raises nitrite concentrations in near-wall layers of flowing blood, resulting in cumulative NO concentrations in downstream arterioles sufficient for vasodilation.Confirmation and implicationsUtilizing a multiscale model of nitrite transport in bifurcating arteries, we tested the hypothesis for femoral artery flow under resting and exercised states of cardiovascular stress. Results indicate intravascular transport of nitrite from upstream endothelium could result in vasodilator-active levels of nitrite in downstream resistance vessels. The hypothesis could be confirmed utilizing artery-on-a-chip technology to measure NO production rates directly and help validate numerical model predictions. Further characterization of this mechanism may improve our understanding of symptomatic peripheral artery occlusive disease and exercise physiology.

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Section: Methodsmentioning

confidence: 99%

Transport of nitrite from large arteries modulates regional blood flow during stress and exercise

Babbs

Goergen

Rayz

2023

Front. Cardiovasc. Med.

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“…MD simulations and free energy calculations indicate that CO and NO behave similarly in the CHOL-rich membrane environment [111]. Nevertheless, the diffusion of NO through biological systems is fast (2.2 × 10 −5 cm 2 /s) [112][113][114] and, moreover, the process is likely facilitated by aquaporins [73]. Thus, gaseous NTs should be able to diffuse and bind into their specific receptors regardless of their positioning in respect to the lipid bilayer.…”

Section: The Effect Of Membrane Lipid Composition On Neurotransmittermentioning

confidence: 99%

A Perspective: Active Role of Lipids in Neurotransmitter Dynamics

Postila

Róg

2019

Mol Neurobiol

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Synaptic neurotransmission is generally considered as a function of membrane-embedded receptors and ion channels in response to the neurotransmitter (NT) release and binding. This perspective aims to widen the protein-centric view by including another vital component-the synaptic membrane-in the discussion. A vast set of atomistic molecular dynamics simulations and biophysical experiments indicate that NTs are divided into membrane-binding and membrane-nonbinding categories. The binary choice takes place at the water-membrane interface and follows closely the positioning of the receptors' binding sites in relation to the membrane. Accordingly, when a lipophilic NT is on route to a membrane-buried binding site, it adheres on the membrane and, then, travels along its plane towards the receptor. In contrast, lipophobic NTs, which are destined to bind into receptors with extracellular binding sites, prefer the water phase. This membrane-based sorting splits the neurotransmission into membraneindependent and membrane-dependent mechanisms and should make the NT binding into the receptors more efficient than random diffusion would allow. The potential implications and notable exceptions to the mechanisms are discussed here. Importantly, maintaining specific membrane lipid compositions (MLCs) at the synapses, especially regarding anionic lipids, affect the level of NT-membrane association. These effects provide a plausible link between the MLC imbalances and neurological diseases such as depression or Parkinson's disease. Moreover, the membrane plays a vital role in other phases of the NT life cycle, including storage and release from the synaptic vesicles, transport from the synaptic cleft, as well as their synthesis and degradation.

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Lung Diffusing Capacities (D_L) for Nitric Oxide (NO) and Carbon Monoxide (CO): The Evolving Story

Borland¹,

Hughes²

2019

Comprehensive Physiology

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Permeability and diffusivity of nitric oxide in human plasma and red cells

Cited by 6 publications

References 23 publications

Transport of nitrite from large arteries modulates regional blood flow during stress and exercise

Transport of nitrite from large arteries modulates regional blood flow during stress and exercise

A Perspective: Active Role of Lipids in Neurotransmitter Dynamics

Lung Diffusing Capacities (D_L) for Nitric Oxide (NO) and Carbon Monoxide (CO): The Evolving Story

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Permeability and diffusivity of nitric oxide in human plasma and red cells

Cited by 6 publications

References 23 publications

Transport of nitrite from large arteries modulates regional blood flow during stress and exercise

Transport of nitrite from large arteries modulates regional blood flow during stress and exercise

A Perspective: Active Role of Lipids in Neurotransmitter Dynamics

Lung Diffusing Capacities (DL) for Nitric Oxide (NO) and Carbon Monoxide (CO): The Evolving Story

Contact Info

Product

Resources

About

Lung Diffusing Capacities (D_L) for Nitric Oxide (NO) and Carbon Monoxide (CO): The Evolving Story